Osteonecrosis in a chemically induced rat model of human hemolytic disorders associated with thrombosis--a new model for avascular necrosis of bone.


:Bone injury occurs in human hemolytic disorders associated with thrombosis, such as beta-thalassemia and sickle cell disease. Exposure of rats to 2-butoxyethanol (BE) has been associated with hemolytic anemia, disseminated thrombosis, and infarction in multiple organs including bone. This rat model apparently mimics acute hemolysis and thrombosis in humans. To elucidate the extent of bone injury, male and female Fischer F344 rats were given 4 daily doses of 250 mg BE/5 ml water/kg of body weight. Tail vertebrae were studied by histopathology and magnetic resonance imaging (MRI). Thrombosis and infarction were seen in both sexes, but females were more severely affected. Lesions were characterized by extensive medullary fat necrosis, granulomatous inflammation, fibroplasia, growth plate degeneration, and new woven bone formation adjacent to necrotic bone trabeculae. MRI mean and standard deviation tissue-density data for both sexes indicated a significant (P < or = 0.05) decrease following 4-days treatment and a significant increase (P < or = 0.05) following an additional 24 days without treatment. Thus, MRI was useful in revealing BE-induced bone injury, which was predominantly necrotic initially and subsequently regenerative with proliferation of connective tissue and bone following postischemia recovery.


Calcif Tissue Int


Shabat S,Nyska A,Long PH,Goelman G,Abramovitch R,Ezov N,Levin-Harrus T,Peddada S,Redlich M,Yedgar S,Nyska M




Has Abstract


2004-03-01 00:00:00












  • Effect of a hypergravity environment on cortical bone elasticity in rats.

    abstract::There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Kohles SS,Bowers JR,Vailas AC,Vanderby R Jr

    更新日期:1996-09-01 00:00:00

  • Developing antler, a model for endocrine regulation of bone growth. Concentration gradient of T3, T4, and alkaline phosphatase in the antler, jugular, and the saphenous veins.

    abstract::Thyroxine (T4), triiodothyronine (T3), and alkaline phosphatase (AP) were assayed monthly in white-tailed deer plasma obtained from the antler (A), jugular (J), and the saphenous (S) veins during the period of antler growth and the period of mineralization. The concentrations of T3, T4, and AP in the J and S were not ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Bubenik GA,Sempere AJ,Hamr J

    更新日期:1987-07-01 00:00:00

  • Effect of 1 alpha-hydroxycholecalciferol on psoriasis vulgaris: a pilot study.

    abstract::We carried out a clinical trial of 1 alpha-hydroxycholecalciferol [1 alpha(OH)D3] at a dose of 1.0 microgram a day on 7 patients with psoriasis vulgaris. These patients had been treated by topical applications of corticosteroids before this study without improvement, and during the clinical trial, treatment of topical...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Takamoto S,Onishi T,Morimoto S,Imanaka S,Yukawa S,Kozuka T,Kitano Y,Seino Y,Kumahara Y

    更新日期:1986-12-01 00:00:00

  • Longitudinal Change in Peripheral Quantitative Computed Tomography Assessment in Older Adults: The Hertfordshire Cohort Study.

    abstract::There are few longitudinal data on change in bone structure and muscle mass, strength and function in later life. We report these, and consider bone-muscle interrelationships in older men and women. We studied 188 men and 166 women from the Hertfordshire Cohort Study, who underwent peripheral quantitative computed tom...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Patel A,Edwards MH,Jameson KA,Ward KA,Fuggle N,Cooper C,Dennison EM

    更新日期:2018-11-01 00:00:00

  • Role of oestrogen in the development of osteoporosis.

    abstract::The evidence now strongly supports a central role for oestrogen in the maintenance of the normal female skeleton. Loss of endogenous oestrogen leads to a period of accelerated bone loss which can be prevented with appropriate oestrogen replacement. The route of administration is not important provided adequate dosages...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审


    authors: Hillard TC,Stevenson JC

    更新日期:1991-01-01 00:00:00

  • X-ray diffraction of the calcified tissues in Polypterus.

    abstract::Pyrolyzed scales, fin spines, and bone from the ray-finned bony fish Polypterus (Actinopterygii) showed two mineral phases on X-ray diffraction: hydroxyapatite (HA), Ca5(PO4)3OH, and whitlockite, Ca3(PO4)2. The ratio of HA/whitlockite varied with the structure (scale, spine, bone) within each individual fish. The rela...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Meinke DK,Skinner HC,Thomson KS

    更新日期:1979-08-24 00:00:00

  • Buserelin-mediated osteoporosis: effects of restoring estrogen on bone resorption and whole body calcium content in the rat.

    abstract::In the rat, prolonged administration of the luteinizing, hormone-releasing hormone agonist buserelin (25 micrograms/kg body wt/day s.c.) lowers blood estradiol, raises bone resorption, and induces osteopenia. The present study was undertaken to determine whether withdrawal of buserelin normalizes blood estradiol, slow...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Goulding A,Gold E

    更新日期:1990-01-01 00:00:00

  • Influence of birth weight on calcaneal bone stiffness in Belgian preadolescent children.

    abstract::The aim of this study was to investigate the relation between birth weight and calcaneal bone stiffness in a large sample of Belgian, healthy, preadolescent children. Participants were 827 children (3.6-11.2 years, 51.6 % boys) from the Belgian cohort of the IDEFICS study. Birth weight was obtained using a parental qu...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Van den Bussche K,Michels N,Gracia-Marco L,Herrmann D,Eiben G,De Henauw S,Sioen I

    更新日期:2012-10-01 00:00:00

  • Whole-Exome Sequencing Identifies Novel Compound Heterozygous ZNF469 Mutations in Two Siblings with Mild Brittle Cornea Syndrome.

    abstract::Connective tissue diseases, including osteogenesis imperfecta (OI) and Ehlers-Danlos syndrome (EDS), exhibit a high degree of clinical and genetic heterogeneity. We report two sisters with blue sclerae, joint hypermobility and hearing loss. Whole-exome sequencing identified two compound heterozygous ZNF469 loss-of-fun...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Rolvien T,Kornak U,Linke SJ,Amling M,Oheim R

    更新日期:2020-09-01 00:00:00

  • Molecular structure at the bone-implant interface: a vibrational spectroscopic characterization.

    abstract::Test implant plates surgically retrieved from distal femurs of dogs were studied by Raman spectroscopy in order to characterize the bone-implant interface. The implant surface consisted of phosphate mineral, plasma sprayed on a titanium substrate. On the basis of its spectroscopic signature, the phosphate mineral of b...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Walters MA,Blumenthal NC,Leung Y,Wang Y,Ricci JL,Spivak JM

    更新日期:1991-05-01 00:00:00

  • A phorbol ester induces secretion of alkaline phosphatase activity in human osteosarcoma cells.

    abstract::The phorbol ester 12-O-tetradecanoyl-13-acetate (TPA) blocked the growth of, and induced the appearance of processes in the human osteosarcoma cell line U-2 OS. The phorbol ester decreased the intracellular level of alkaline phosphatase (APase) activity (as measured per mg cell protein) and caused a marked increase in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Ringbom-Anderson T,Akerman KE

    更新日期:1992-06-01 00:00:00

  • Ossification centers of human femur.

    abstract::The purpose of the present work was to investigate the appearance of ossification centers in human femur by Dual-energy X-ray Absorptiometry (DXA), comparing densitometric data with morphogenetic events. Posteroanterior scans were performed on 31 dried femora (from 11.5 weeks of conceptual age to 1 year of postnatal l...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Panattoni GL,D'Amelio P,Di Stefano M,Isaia GC

    更新日期:2000-04-01 00:00:00

  • Low Relative Lean Mass is Associated with Increased Likelihood of Abdominal Aortic Calcification in Community-Dwelling Older Australians.

    abstract::Age-related loss of skeletal muscle is associated with increased risk of functional limitation and cardiovascular (CV) mortality. In the elderly abdominal aortic calcification (AAC) can increase CV risk by altering aortic properties which may raise blood pressure and increase cardiac workload. This study investigated ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Rodríguez AJ,Scott D,Khan B,Khan N,Hodge A,English DR,Giles GG,Ebeling PR

    更新日期:2016-10-01 00:00:00

  • Potential Role of DARC-Chemokine Interaction in the Recruitment of Osteoclast Precursors in Response to Bacterial Lipopolysaccharide Challenge.

    abstract::Duffy antigen receptor for chemokines (DARC) binds to a number of pro-inflammatory chemokines, and since chemokines are known to regulate trafficking of osteoclast (OC) precursors, we predicted that DARC would regulate OC recruitment to sites of inflammation by modulating chemokine activity. To test this hypothesis, w...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Alemi F,Elgendy M,Edderkaoui B

    更新日期:2016-11-01 00:00:00

  • Apatite mineralization in teeth of the chiton Acanthopleura echinata.

    abstract::Raman spectroscopy has been used to demonstrate, for the first time, that calcium mineralization in the core of the major lateral teeth of the chiton Acanthopleura echinata takes place as an ordered process, with crystalline carbonated apatite being the first mineral deposited. Deposition begins at the top of the toot...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Lee AP,Brooker LR,Macey DJ,van Bronswijk W,Webb J

    更新日期:2000-11-01 00:00:00

  • No effect of rosuvastatin in the zoledronate-induced acute-phase response.

    abstract::The acute-phase response (APR) is frequently observed in patients treated with intravenous (iv) zoledronate (ZOL). We investigated whether a short course of rosuvastatin (ROSU) could attenuate the ZOL--induced APR through blocking the mevalonate pathway at a proximal level. Twenty-eight osteoporotic postmenopausal wom...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,随机对照试验


    authors: Makras P,Anastasilakis AD,Polyzos SA,Bisbinas I,Sakellariou GT,Papapoulos SE

    更新日期:2011-05-01 00:00:00

  • Regional measurement of bone calcium accretion rate and exchangeable pool with a whole-body counter: method and studies in subjects without bone disease.

    abstract::A whole-body counter was used to measure the retention and distribution of radioactivity along the longitudinal axis of the body during 10 days after the intravenous injection of 50 microCi of 47Ca. These data and the simultaneous measurements of the serum specific activity allowed us to calculate, by the Bauer-Carlss...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Bergmann P,Paternot T,Schoutens A

    更新日期:1983-01-01 00:00:00

  • CYP3A7*1C polymorphism, serum dehydroepiandrosterone sulfate level, and bone mineral density in postmenopausal women.

    abstract::The CYP3A7 enzyme metabolizes some steroid hormones, including dehydroepiandrosterone sulfate (DHEAS). The age-related decline of serum DHEAS levels is believed to contribute to osteoporosis. Previously, the CYP3A7*1C polymorphism has been shown to cause a persistent high CYP3A7 enzyme activity, resulting in lower lev...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Bácsi K,Kósa JP,Borgulya G,Balla B,Lazáry A,Nagy Z,Horváth C,Speer G,Lakatos P

    更新日期:2007-03-01 00:00:00

  • Bone progenitor cell deficits and the age-associated decline in bone repair capacity.

    abstract::Aging bone shows a progressive decline in mass and strength. Previous studies have suggested that bone marrow stem cells are reduced with aging and that this could be responsible, in part, for age-associated bone deficits. We measured the number of osteoprogenitor cells present in the bone marrow from adult and aged r...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Quarto R,Thomas D,Liang CT

    更新日期:1995-02-01 00:00:00

  • Effect of static loading of dentin beams at various pH levels.

    abstract::Noncarious cervical lesions have a multifactorial etiology. Mechanical stress has been identified as one of the factors, but little evidence exists for its cause-effect relationship. This study was conducted at three different pH levels to observe the surface loss on dentin beams under tension and compression. Bovine ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Mishra P,Palamara JE,Tyas MJ,Burrow MF

    更新日期:2006-12-01 00:00:00

  • Absence of glutamine supplementation prevents differentiation of murine calvarial osteoblasts to a mineralizing phenotype.

    abstract::Osteoblasts in vitro differentiate from a proliferating to a mineralizing phenotype upon transfer to a medium rich in beta-glycerophosphate and ascorbic acid. The nutritional requirements of the cells at different stages of this differentiation process are not known. In other cell types, nutritional supplementation du...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Brown PM,Hutchison JD,Crockett JC

    更新日期:2011-12-01 00:00:00

  • Gender-specific associations of appendicular muscle mass with BMD in elderly Italian subjects.

    abstract::Currently used diagnostic measures for sarcopenia are based on the evaluation of appendicular skeletal muscle mass (ASMM) divided by height-squared (ASMMI). This study aimed to investigate the associations between different operational definitions of appendicular muscle mass and BMD at different skeletal sites in agin...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Gonnelli S,Caffarelli C,Cappelli S,Rossi S,Giordano N,Nuti R

    更新日期:2014-10-01 00:00:00

  • Phosphorus-31 in vivo magnetic resonance spectroscopy of bone fails to diagnose osteoporosis.

    abstract::The values of in vivo T1 relaxation time (T1) of phosphorus atoms of wrist bone have been measured by phosphorus-31 magnetic resonance spectroscopy (MRS) in 65 menopausal women separated into three groups: (1) age-matched women without any paraclinical or clinical osteoporosis; (2) patients with paraclinical osteoporo...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Confort-Gouny S,Mattéi JP,Vion-Dury J,Roux H,Bisset JP,Cozzone PJ

    更新日期:1995-06-01 00:00:00

  • Absence of correlation between BMP-4 polymorphism and postmenopausal osteoporosis in Italian women.

    abstract::Several studies have demonstrated that bone has the power of regeneration and repair. BMPs (bone morphogenetic proteins) are involved in the determination of osteoblast phenotype and bone turnover, therefore genes coding for these proteins, like BMP-4, could be considered potential candidate genes for osteoporosis. We...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Semprini S,Mango R,Brancati F,Dallapiccola B,Becherini L,Novelli G,De Lorenzo A,Brandi ML,Gennari L

    更新日期:2000-07-01 00:00:00

  • Congenital Conditions of Hypophosphatemia Expressed in Adults.

    abstract::The main congenital conditions of hypophosphatemia expressed in adulthood include several forms of hereditary hypophosphatemic rickets and a congenital disorder of vitamin D metabolism characterized by osteomalacia and hypophosphatemia in adult patients. Hypophosphatemia in adults is defined as serum phosphate concent...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审


    authors: Marcucci G,Brandi ML

    更新日期:2021-01-01 00:00:00

  • A ChIP-seq-defined genome-wide map of MEF2C binding reveals inflammatory pathways associated with its role in bone density determination.

    abstract::Genome-wide association studies (GWAS) have demonstrated that genetic variation at the MADS box transcription enhancer factor 2, polypeptide C (MEF2C) locus is robustly associated with bone mineral density, primarily at the femoral neck. MEF2C is a transcription factor known to operate via the Wnt signaling pathway. O...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Johnson ME,Deliard S,Zhu F,Xia Q,Wells AD,Hankenson KD,Grant SF

    更新日期:2014-04-01 00:00:00

  • Fracture risk associated with different types of oral corticosteroids and effect of termination of corticosteroids on the risk of fractures.

    abstract::We conducted a case-control study on fracture risk associated with the use of orally administered prednisolone/prednisone, budesonide, methylprednisolone, and hydrocortisone to assess if the various preparations were associated with different fracture patterns. Cases were all subjects with any fracture sustained durin...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Vestergaard P,Rejnmark L,Mosekilde L

    更新日期:2008-04-01 00:00:00

  • Subregional DXA-derived vertebral bone mineral measures are stronger predictors of failure load in specimens with lower areal bone mineral density, compared to those with higher areal bone mineral density.

    abstract::Measurement of areal bone mineral density (aBMD) in intravertebral subregions may increase the diagnostic sensitivity of dual-energy X-ray absorptiometry (DXA)-derived parameters for vertebral fragility. This study investigated whether DXA-derived bone parameters in vertebral subregions were better predictors of verte...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Briggs AM,Perilli E,Codrington J,Reynolds KJ,Parkinson IH,Wark JD

    更新日期:2014-08-01 00:00:00

  • HNGF6A Inhibits Oxidative Stress-Induced MC3T3-E1 Cell Apoptosis and Osteoblast Phenotype Inhibition by Targeting Circ_0001843/miR-214 Pathway.

    abstract::Humanin (HN), a mitochondrial derived peptide, plays cyto-protective role under various stress. In this study, we aimed to investigate the effects of HNGF6A, an analogue of HN, on osteoblast apoptosis and differentiation and the underlying mechanisms. Cell proliferation of murine osteoblastic cell line MC3TC-E1 was ex...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Zhu X,Zhao Z,Zeng C,Chen B,Huang H,Chen Y,Zhou Q,Yang L,Lv J,Zhang J,Pan D,Shen J,Duque G,Cai D

    更新日期:2020-05-01 00:00:00

  • Developmental expression of genes in chick growth cartilage detected by in situ hybridization.

    abstract::We have used in situ hybridization to examine expression of collagen type I, II, and X mRNA and osteonectin mRNA in the chick epiphysis. Tissue samples from the proximal tibial growth cartilage were fixed in modified Carnoy's solution, dehydrated in ethanol, and embedded in paraffin. Longitudinal and transverse sectio...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Oshima O,Leboy PS,McDonald SA,Tuan RS,Shapiro IM

    更新日期:1989-09-01 00:00:00