Abstract:
:Radioiodination of synthetic human 37-49 osteocalcin requires optimal labeling conditions in order to obtain a maximum of mono- and di-iodinated tracer with little contamination by tri- and tetra-iodinated products or "radio-damage." The antibody raised against osteocalcin(h37-49) had the highest affinity for the C-terminal peptide used for iodination and the larger peptide (h30-49). The intact bovine osteocalcin (b1-49) revealed less immunoreactivity. This C-terminal specific radioimmunoassay detected the intact human osteocalcin in HPLC purified plasma and peritoneal dialysate from patients with terminal renal insufficiency and in extracted human bone. Some quantities of osteocalcin peptides with a higher hydrophobicity were predominantly detected in uremic plasma. These peptides that had a higher molecular weight than the intact human molecule might represent aggregated forms of the intact bone-derived osteocalcin. Immunoreactivity in plasma samples from healthy individuals revealed a remarkable difference as to which substance was employed for anticoagulation. Compared to heparin, the addition of EDTA largely reduced the osteocalcin immunoreactivity, implying that conformational changes within the N-terminal portion (containing the Gla- and Cys-residues) are extended to the C-terminal portion.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Jüppner H,Schettler T,Giebel G,Wenner S,Hesch RDdoi
10.1007/BF02555196subject
Has Abstractpub_date
1986-11-01 00:00:00pages
310-5issue
5eissn
0171-967Xissn
1432-0827journal_volume
39pub_type
杂志文章abstract::We examined the role of sleep, growth hormone (GH), and parathyroid hormone [PTH(1-84)] as regulators of the diurnal rhythm of the osteoblastic bone marker, serum osteocalcin (OC). Nine normal subjects were followed with hourly blood sampling during one 24-hour period with normal sleep pattern, and one 24-hour period ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555845
更新日期:1991-12-01 00:00:00
abstract::The existence of nutritional deficiency rickets among infants in sunny Riyadh was confirmed radiologically. Most of the rachitic infants were breast-fed, some received unsupplemented infant feeding formulae, and all live in an environment that is devoid of sunlight. Their mean age at the time of onset was 10.5 months....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02405328
更新日期:1984-05-01 00:00:00
abstract::Raf-1, the product of proto-oncogene c-raf-1, has key roles in the signal transduction pathways within the cell. The molecular mechanisms of tooth development in the mouse embryo are not known in detail. We examined the expression of Raf-1 during subsequent tooth development by immunohistochemical analysis. In mouse e...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02509547
更新日期:1996-01-01 00:00:00
abstract::GNAS is one of the most complex gene loci in the human genome and encodes multiple gene products including Gsα, XLαs, NESP55, A/B, and AS transcripts. XLαs, the extra-large G protein ɑ-subunit, is paternally expressed. XLɑs and Gsɑ share the common 2-13 exons with different promoters and first exons. Therefore, XLɑs c...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-020-00714-2
更新日期:2020-09-01 00:00:00
abstract::Conventional transmission electron microscopy requires an effective dehydration of the tissue specimens under investigation. This dehydration, for which ethanol most often is used, may influence the solubility of the slightly soluble calcium phosphates. To study the behavior of calcium phosphates in ethanol, stable ne...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554840
更新日期:1985-03-01 00:00:00
abstract::The effect of parathormone (PTH), lipopolysaccharide (LPS), or interleukin-1 (IL-1) on calcium release and collagen degradation in bone was examined in vitro using labeled neonatal calvaria of normal mice and also of osteopetrotic microphthalmic (mi/mi) mice that have defective osteoclasts. All three agents stimulated...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554878
更新日期:1985-05-01 00:00:00
abstract::The ability of 1,25(OH)2D3 and of 24,25(OH)2D3 to prevent or to heal rickets in chicks was evaluated by studies of plasma biochemistry, growth plate histology, bone morphometry and microradiography, and bone mineralization. 1,25(OH)2D3 at a dose of 100 ng/day produced fewest abnormalities compared with vitamin D3-trea...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02405303
更新日期:1984-01-01 00:00:00
abstract::The drawbacks of estrogen restrict the clinical use of hormone replacement therapy, and it would be most helpful to explore new estrogenic substances that could prevent bone loss and be free from any adverse effects. We synthesized a new compound named bone-seeking estrogen (SE2) by combining 17β-estradiol (E2) with i...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-013-9739-1
更新日期:2013-08-01 00:00:00
abstract::Five years ago we reported results from a cross-sectional study of the effect of nutritional factors on calcium-regulating hormones and bone loss in perimenopausal women. We found an inverse correlation between serum 25-hydroxyvitamin D (25OHD) and immunoreactive parathyroid hormone (PTH), and we postulated that over ...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
doi:10.1007/BF00334543
更新日期:1992-09-01 00:00:00
abstract::The purpose of this study was to determine the efficacy of using bone mineral measurements of the calcaneus to evaluate osteoporosis. Dual energy X-ray absorptiometry (DXA) of the calcaneus was compared with posteroanterior lumbar absorptiometry (DXA) and vertebral quantitative computed tomography (QCT) measurements i...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298738
更新日期:1995-01-01 00:00:00
abstract::We have recently reported that risedronate preserves normal bone formation and decreases bone remodeling in women with postmenopausal osteoporosis after 3 years of treatment. We report now the results of a 2-year extension study. The primary objective of this study was to determine the effect of 5 years of risedronate...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00223-004-0039-7
更新日期:2004-12-01 00:00:00
abstract::The marrow stromal fibroblast (MSF) population has been shown to include precursor cells for at least five types of connective tissue: bone, cartilage, adipose tissue, fibrous tissue, and hematopoiesis-supporting reticular stroma. In this study, growth requirements for MSF colony formation were studied in vitro. In or...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900121
更新日期:1996-10-01 00:00:00
abstract::The extractions of 85Sr2+, 18F-, sucrose-14C, EDTA-51Cr, and antipyrine-14C in bone were determined by the multiple indicator-dilution method. Fluoride and strontium extractions were 18 to 70% during a single transcapillary passage, and those of EDTA and sucrose were from 11 to 59%, whereas extraction of antipyrine wa...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02407178
更新日期:1980-01-01 00:00:00
abstract::As tobacco smoking has been identified as a risk factor in the development of osteoporosis, possible deleterious effects of nicotine inhalation on bone mineral density (BMD) and mechanical properties of the femur in female rats were studied. Female Sprague Dawley rats were exposed to nicotine vapour 20 hours a day 5 d...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900692
更新日期:1999-09-01 00:00:00
abstract::Vascular calcification (VC), which is associated with high cardiovascular morbidity and mortality in patients with chronic kidney disease, is promoted by the osteoblastic differentiation of vascular smooth muscle cells (VSMCs). The present study explored the functional roles and molecular mechanisms of the long noncod...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00696-1
更新日期:2020-07-01 00:00:00
abstract::Studies of the effect of parathyroidectomy (PTX) on bone turnover in patients with the combination of primary hyperparathyroidism (PHPT) and Paget's disease (PD) are largely limited to case reports. The etiology of the combination is disputed. We report 30 patients and their biochemical (n = 17) and histomorphometric ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900728
更新日期:1999-12-01 00:00:00
abstract::Bone involvement, mainly osteoporosis but also osteosclerosis, is frequent in patients with indolent systemic mastocytosis (ISM). The recent characterization of the canonical Wnt/β-catenin pathway in the regulation of bone remodeling provided important insights for our understanding of the pathophysiology of a number ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-015-9969-5
更新日期:2015-05-01 00:00:00
abstract::Alkaline phosphatase is detected in both soluble and particulate fractions of rat bone. The concentration of alkaline phosphatase in the particulate fraction of rat skull cap bone and femur is high during the peak period of calcification of these bones suggesting the possibility of using it as a marker for the rate of...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555116
更新日期:1986-09-01 00:00:00
abstract::We conducted a case-control study on fracture risk associated with the use of orally administered prednisolone/prednisone, budesonide, methylprednisolone, and hydrocortisone to assess if the various preparations were associated with different fracture patterns. Cases were all subjects with any fracture sustained durin...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-008-9124-7
更新日期:2008-04-01 00:00:00
abstract::We treated four hypercalcemic cancer patients by nasal hCT, 3 x 2 mg daily, which has been reported to be active in Paget's disease at lower doses. Only one patient became normocalcemic and mean (+/- SEM) calcium levels fell from 11.6 +/- 0.2 mg/dl before therapy to 10.7 +/- 0.6 mg/dl 2-3 days after starting hCT. The ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00296210
更新日期:1992-07-01 00:00:00
abstract::Methotrexate (MTX) is one of the most commonly prescribed drugs for autoimmune rheumatic diseases. As there is no consensus on its negative effects on bone, the purpose of this investigation was to determine the clinical spectrum of patients with stress fractures due to long-term MTX treatment (i.e., MTX osteopathy). ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00765-5
更新日期:2021-02-01 00:00:00
abstract::The purpose of this research was to address the following question: How is the solubility of fluoride-pretreated carbonated apatite (CAP) in aqueous acidic media related to the equilibrium solution fluoride and/or the CAP adsorbed fluoride levels? A CAP sample prepared by a precipitation method at 70 degrees C contain...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-1069-z
更新日期:2003-03-01 00:00:00
abstract::In this study, we demonstrated that type I collagen matrix induced the expression of osteoblastic phenotypes of bone marrow cells, and that antibone sialoprotein (BSP) monoclonal antibody suppressed the expression of these phenotypes. On the other hand, BSP accelerated the expression of osteoblastic phenotypes of bone...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010078
更新日期:2000-05-01 00:00:00
abstract::The present study was designed to characterize dentin formed in transplanted rat molars by investigating calcium (Ca), phosphorus (P), and magnesium (Mg) concentrations using electron probe microanalysis (EPMA) as well as examining the rate of dentin matrix formation by vital staining. The unerupted immature lower rig...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0134-4
更新日期:2006-03-01 00:00:00
abstract::Octacalcium phosphate (Ca8H2(PO4)6 . 5H2O) is considered to be precursor in the formation of apatite in bones and teeth; a crucial step for incorporation of impurities appears to occur during its hydrolysis. The present study examines the role that octacalcium phosphate plays in the process of incorporation of carbona...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02408521
更新日期:1980-01-01 00:00:00
abstract::There are few studies of the effect of smoking on bone density in young women. The reported antiestrogenic effect of smoking could be a mechanism for a possible effect of smoking on bone. We measured bone mineral density (BMD) by dual-energy X-ray absorptiometry (whole body, proximal femur, lumbar spine), and serum le...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00301626
更新日期:1992-04-01 00:00:00
abstract::The definition of osteoporosis was based for several years on bone mineral density values, which were used by most guidelines for defining treatment thresholds. The availability of tools for the estimation of fracture risk, such as FRAX™ or its adapted Italian version, DeFRA, is providing a way to grade osteoporosis s...
journal_title:Calcified tissue international
pub_type: 社论
doi:10.1007/s00223-013-9748-0
更新日期:2013-09-01 00:00:00
abstract::Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe destructive arthropathy. BCP crystals induce the secretion of matrix-degrading enzymes such as collagenase. No prophylactic or therapeutic agents are recognized to ameliorate the cartilage damage associated with BCP deposits in joints. As...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00571332
更新日期:1993-06-01 00:00:00
abstract::Cross-sectional and case-control studies are efficient and inexpensive approaches to the study of osteoporosis; their validity depends primarily on how the subjects are sampled. Retrospective studies of fractures may be biased if measurements change as a result of the fracture. Prospective cohort studies are less pron...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/BF02555081
更新日期:1991-01-01 00:00:00
abstract::Magnetic resonance imaging (MRI) is commonly used in the assessment of the musculoskeletal system and associated pathology. The ability of MRI to measure the signals from water and lipid protons enables quantitative measurements of bone porosity. The goal of this investigation was to demonstrate that the density and c...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230050015
更新日期:2000-01-01 00:00:00