Abstract:
:The drawbacks of estrogen restrict the clinical use of hormone replacement therapy, and it would be most helpful to explore new estrogenic substances that could prevent bone loss and be free from any adverse effects. We synthesized a new compound named bone-seeking estrogen (SE2) by combining 17β-estradiol (E2) with iminodiacetic acid through the Mannich reaction. E2 and SE2 were labeled with isotope (3)H, and the tissue distribution tests of E2-(3)H and SE2-(3)H were analyzed by the radioactivity. The specific nuclear binding of E2 and SE2 in osteoblasts was measured. SE2 exhibited significantly greater affinity for bone but lower affinity for ovary and uterus than did E2, and SE2 maintained a high affinity for the estrogen receptor alpha similar to that of E2. SE2 administration did not induce uterine hypertrophy. Body weight increase was significantly suppressed by treatment with E2 but not by SE2 after ovariectomy (OVX). SE2 decreased bone turnover as E2 after OVX detected by serum biochemical markers. Bone histology and micro-CT analysis revealed that SE2 administration, similar to E2, could improve bone mass and trabecular architecture after OVX. Biomechanical analyses showed that SE2 treatment effectively increased mechanical properties after OVX. The results suggested that SE2 was effective in preventing OVX-induced bone loss and exhibited few side effects on body weight and uterine hypertrophy, which was beneficial in reducing the adverse effects caused by E2. SE2 may be a better choice than E2 for the prevention of postmenopausal osteoporosis.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Zhao Q,Liu X,Zhang L,Shen X,Qi J,Wang J,Qian N,Deng Ldoi
10.1007/s00223-013-9739-1subject
Has Abstractpub_date
2013-08-01 00:00:00pages
172-83issue
2eissn
0171-967Xissn
1432-0827journal_volume
93pub_type
杂志文章abstract::Diets with a high saturated fat content can produce deleterious effects on the absorption of dietary calcium and consequently an adverse effect on bone mineralization in growing animals. Hence dietary fat may have long-term consequences for skeletal health and skeletal pathologies such as osteoporosis. Whether a diet ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900492
更新日期:1998-07-01 00:00:00
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journal_title:Calcified tissue international
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abstract::Binding sites for wheat germ agglutinin (WGA)-lectin have been shown to become revealed in the demineralized resorption lacunae that osteoclasts excavate on bone substrate. Peroxidase-conjugated WGA-lectin, which binds to bone matrix glycoconjugates and proteoglycans, was used in pit formation assays to assess the act...
journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02408543
更新日期:1980-01-01 00:00:00
abstract::We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Gr...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-003-0110-9
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journal_title:Calcified tissue international
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更新日期:2000-10-01 00:00:00
abstract::We have recently reported that risedronate preserves normal bone formation and decreases bone remodeling in women with postmenopausal osteoporosis after 3 years of treatment. We report now the results of a 2-year extension study. The primary objective of this study was to determine the effect of 5 years of risedronate...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2004-12-01 00:00:00
abstract::The present study was undertaken to determine the fluoride distribution profile in the rat femur. Male Wistar rats were divided into four groups and given water containing 0, 25, 50, and 100 ppm of fluoride, respectively, for 10 weeks. The fluoride distribution from the periosteum to the endosteum was determined in ea...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555045
更新日期:1990-03-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02441163
更新日期:1979-03-13 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-015-9981-9
更新日期:2015-06-01 00:00:00
abstract::We compared indices of three-dimensional microstructure of iliac trabecular bone between 26 patients with vertebral compression fractures due to postmenopausal osteoporosis and 24 control subjects without vertebral fracture, who were matched for age, sex, race, menopausal status, and several densitometric and histolog...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554913
更新日期:1985-12-01 00:00:00
abstract::Fourier-transform (FT) Raman spectroscopy was used to characterize the organic and mineral components of biological and synthetic calcium phosphate minerals. Raman spectroscopy provides information on biological minerals that is complimentary to more widely used infrared methodologies as some infrared-inactive vibrati...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00305529
更新日期:1994-05-01 00:00:00
abstract::Normative bone mineral density (BMD) and bone mineral content (BMC) values for the total body (TB), proximal femur (PF), and antero-posterior lumbar spine (LS) were obtained from a large cross-sectional sample of children and adolescents who were 8-17 years of age. There were 977 scans for the TB, 892 for the PF, and ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900138
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abstract::The acute-phase response (APR) is frequently observed in patients treated with intravenous (iv) zoledronate (ZOL). We investigated whether a short course of rosuvastatin (ROSU) could attenuate the ZOL--induced APR through blocking the mevalonate pathway at a proximal level. Twenty-eight osteoporotic postmenopausal wom...
journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
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更新日期:2011-05-01 00:00:00
abstract::Cortical bone can be modeled as a complex hierarchical composite interrelating both structure and material properties on four levels of structural organization: molecular, ultrastructural, microscopic, and macroscopic. In young animals, the microstructural systems are long parallel lamellar units, plexiform bone, whic...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02406131
更新日期:1984-01-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-006-0137-9
更新日期:2007-01-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02557688
更新日期:1985-01-01 00:00:00
abstract::Peritubular dentin (PTD) is a relatively dense mineralized tissue surrounding tooth dentin tubules, whose composition and mode of formation are still unclear. Fourier transform infrared microspectroscopic studies of the organic and mineral components of the highly developed horse PTD indicate that the peritubular matr...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-2108-5
更新日期:2002-08-01 00:00:00
abstract::The aim of this study was to test whether the nitric oxide (NO) donor sodium nitroprusside (SNP) has an effect on mineralization in ATDC5 cells. Mineralization in ATDC5 cell culture was induced by addition of beta-glycerophosphate or inorganic phosphate, visualized by staining precipitated calcium with an alizarin red...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-1233-y
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00299308
更新日期:1994-11-01 00:00:00
abstract::Pleiotrophin (PTN) was found to have potent effects on regulation of osteoblast recruitment, proliferation and differentiation. The present study examined the long-term effects of targeted PTN over-expression on bone development and repair in a transgenic mouse model. Femurs and tibiae from the PTN transgenic mice and...
journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章,多中心研究
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abstract::The ability of 1,25(OH)2D3 and of 24,25(OH)2D3 to prevent or to heal rickets in chicks was evaluated by studies of plasma biochemistry, growth plate histology, bone morphometry and microradiography, and bone mineralization. 1,25(OH)2D3 at a dose of 100 ng/day produced fewest abnormalities compared with vitamin D3-trea...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02405303
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abstract::The effect of the configuration of porous cylindrical hydroxyapatite (HA) scaffold and laminin preparation of the scaffold on bone formation was estimated. HA scaffolds with a hollow center of 2 or 4 mm in diameter and those without a hollow center were used. The scaffolds were immersed in laminin solution or in cultu...
journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554833
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abstract::The effects of 5, 10, and 20% dietary xylitol supplementations on the resorption of bone were studied. The resorption was measured by the urinary excretion of [3H] radioactivity from [3H]tetracycline-prelabeled rats. The 10 and 20% oral xylitol administrations caused a significant decrease in the excretion of [3H] as ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298616
更新日期:1995-03-01 00:00:00
abstract::Quantitative trait locus analysis can be used to identify genes critically involved in biological processes. No such analysis has been applied to identifying genes that control bone fracture healing. To determine the feasibility of such an approach, healing of femur fractures was measured between C57BL/6, DBA/2, and C...
journal_title:Calcified tissue international
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00