Abstract：
:Binding sites for wheat germ agglutinin (WGA)-lectin have been shown to become revealed in the demineralized resorption lacunae that osteoclasts excavate on bone substrate. Peroxidase-conjugated WGA-lectin, which binds to bone matrix glycoconjugates and proteoglycans, was used in pit formation assays to assess the activity of isolated osteoclasts cultured on either 3-amino-1,1-hydroxypropylidene-bisphosphonate (APD)-or dichloromethylene bisphosphonate (Cl2MBP)-covered bone slices. Immunofluorescence and histochemical techniques were also used to study the effects of bone-bound bisphosphonates on isolated rat osteoclasts. Neither APD nor Cl2MBP interfered with the special organization of actin or vinculin in osteoclasts when the cells were initializing their resorption cycle. After 24 hours of culture, the number of resorbing osteoclasts increased strongly on control slices, but remained low on either APD- or Cl2MBP-treated slices. At this time, the actin and vinculin rings in osteoclasts also started to exhibit abnormal, more diffuse staining. Both bisphosphonates studied resulted in signs of cytotoxicity: the number of osteoclasts decreased on APD- or Cl2MBP-covered bone during the course of the study and those remaining attached exhibited severe cytoplasmic retractions. The total areas of resorption remained at significantly lower levels in both experimental groups studied, and this was due to decreases in both the number and sizes of individual resorption pits. The size of the most extensive lacunae detected on the Cl2MBP slices did not exceed 5 x 10(3) microns 2, whereas on the control slices, resorption pits bigger than 15 x 10(3) microns 2 were frequently discovered.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Selander K,Lehenkari P,Väänänen HKdoi
10.1007/BF00299317subject
Has Abstractpub_date
1994-11-01 00:00:00pages
368-75issue
5eissn
0171-967Xissn
1432-0827journal_volume
55pub_type
杂志文章abstract：:Psoriatic arthritis (PsA) is an inflammatory rheumatic disorder that occurs in patients with psoriasis and predominantly affects musculoskeletal structures, skin, and nails. The etiology of PsA is not well understood but evidence supports an interplay of genetic, immunologic, and environmental factors which promote pa...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-017-0380-2
更新日期：2018-05-01 00:00:00
abstract：:As the correlation of bone mass from childhood to adulthood is unclear, we conducted a long-term prospective observational study to determine if a pediatric bone mass scan could predict adult bone mass. We measured cortical bone mineral content (BMC [g]), bone mineral density (BMD [g/cm(2)]), and bone width (cm) in th...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-013-9802-y
更新日期：2014-02-01 00:00:00
abstract：:To define the quantitative aspects of ossification of human fetal spine, we performed a high resolution densitometric study by lateral and postero-anterior scanning of five fetal spines (18-36 weeks of conceptual age) and one spine of a 4-month-old infant. The data were plotted against developmental age for each spine...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00299001
更新日期：1995-07-01 00:00:00
abstract：:We assessed the relationship of bone density and microarchitecture between hand, peripheral, and axial skeletal sites using high-resolution peripheral quantitative computed tomography (HR-pQCT) and dual-energy X-ray absorptiometry (DXA) in patients with rheumatoid arthritis (RA) and which factors influence these param...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-012-9644-z
更新日期：2012-11-01 00:00:00
abstract：:This study revealed the distinguished changes of preferential orientation of collagen and apatite and Young's modulus in two different types of osteoporotic bones compared with the normal bone. Little is known about the bone material properties of osteoporotic bones; therefore, we aimed to assess material properties i...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0508-z
更新日期：2019-04-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00760-w
更新日期：2021-02-01 00:00:00
abstract：:Glycogen storage disease type 1a (Von Gierke's disease) is one of the more common glycogen storage diseases (GSD). GSD 1a patients can have severe idiopathic osteopenia, often beginning at a young age. Since calcium tracer studies offer a sensitive probe of the bone microenvironment and of calcium deposition, kinetics...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00369209
更新日期：1996-12-01 00:00:00
abstract：:The effect of ascorbic acid deficiency on bone metabolism was evaluated using the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat model. Ascorbic acid (Asc)-deficient rats gained body weight in a manner similar to Asc-supplemented rats (control) during 3 weeks, but began to lose weight during the 4th week o...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-002-2040-3
更新日期：2003-08-01 00:00:00
abstract：:It has been suggested that oxidative stress is associated with the pathogenesis of osteoporosis. The objective of this study was to explore the association between a marker of oxidative stress and either bone turnover markers or bone mineral density (BMD) in postmenopausal women. In addition, the effects of oxidative ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9393-9
更新日期：2010-09-01 00:00:00
abstract：:Using a histochemical method for demonstrating acid phosphatase activity, we have studied osteoclasts residing at two different bone sites in rat incisor alveolar bone, one at the endosteum and the other at the tooth socket, and compared the response of these osteoclasts to systemic changes. After 12 days of calcium (...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405315
更新日期：1984-03-01 00:00:00
abstract：:The aim of this study was to follow the changes in bone mineral density (BMD) and biochemical markers of bone turnover in 10 children (7.5-17.5 years of age) with severe juvenile idiopathic arthritis (JIA), during a 3-year therapy with salmon calcitonin (100 IU/day 2 months on and 2 off for a year and 200 IU/day for 2...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/s00223-001-0008-3
更新日期：2001-07-01 00:00:00
abstract：:Cross-sectional and case-control studies are efficient and inexpensive approaches to the study of osteoporosis; their validity depends primarily on how the subjects are sampled. Retrospective studies of fractures may be biased if measurements change as a result of the fracture. Prospective cohort studies are less pron...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/BF02555081
更新日期：1991-01-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF01321890
更新日期：1993-08-01 00:00:00
abstract：:Skeletal tissues contain, apart from cells of the osteogenic and chondrogenic lineage, cells of hemopoietic origin, e.g., macrophages, osteoclasts, and their precursors. In the present study we examined the sensitivity for extracellular ATP4- of the above-mentioned cell types in freshly isolated, bone-derived cell pop...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00297190
更新日期：1994-08-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555856
更新日期：1991-12-01 00:00:00
abstract：:Macroporous biphasic calcium phosphate (MBCP) blocks were implanted into rabbit trabecular bone and muscle, recovered 18 weeks later, and then observed and analyzed by transmission electron microscopy (TEM), electron diffraction, and electron microprobes. The results showed that (1) apatitic microcrystals appeared by ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005825
更新日期：1999-05-01 00:00:00
abstract：:Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe destructive arthropathy. BCP crystals induce the secretion of matrix-degrading enzymes such as collagenase. No prophylactic or therapeutic agents are recognized to ameliorate the cartilage damage associated with BCP deposits in joints. As...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00571332
更新日期：1993-06-01 00:00:00
abstract：:Vascular calcification (VC), which is associated with high cardiovascular morbidity and mortality in patients with chronic kidney disease, is promoted by the osteoblastic differentiation of vascular smooth muscle cells (VSMCs). The present study explored the functional roles and molecular mechanisms of the long noncod...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00696-1
更新日期：2020-07-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00308323
更新日期：1993-02-01 00:00:00
abstract：:This cross-sectional, observational study was designed to identify clinical risk factors of osteoporosis and fractures in Korean women to validate the probability of osteoporosis and subsequent fractures. A total of 1541 Korean women were recruited nationally. Fracture history of any site, risk factors of osteoporosis...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9182-x
更新日期：2008-12-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411226
更新日期：1982-03-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-016-0164-0
更新日期：2017-05-01 00:00:00
abstract：:Aluminum ions (Al) and chromium (III) ions (Cr), as they exist in aqueous solution at neutral pH, adsorb onto calcium hydroxyapatite crystals (HAP) and severely inhibit their dissolution process, when present in concentrations less than 1 microM. Iron (III) ions (Fe), at concentrations up to 10 microM, have no effect ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555127
更新日期：1987-07-01 00:00:00
abstract：:The present study was undertaken to determine the fluoride distribution profile in the rat femur. Male Wistar rats were divided into four groups and given water containing 0, 25, 50, and 100 ppm of fluoride, respectively, for 10 weeks. The fluoride distribution from the periosteum to the endosteum was determined in ea...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555045
更新日期：1990-03-01 00:00:00
abstract：:Inorganic phosphate (P(i)) can regulate the level of skeletal alkaline phosphatase (ALP) activity in human osteoblast-like cells, but not by means of changes in transcription or release from the cell surface. The current studies were intended to determine whether (1) P(i) affected the inactivation of ALP activity in h...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00302073
更新日期：1995-11-01 00:00:00
abstract：:Tumor tissue from a patient with squamous cell carcinoma of the lung and hypercalcemia has been serially implanted into athymic mice. Tumor-bearing mice develop cachexia, hypercalcemia without bone metastases, hypophosphatemia, increased urinary cyclic adenosine monophosphate (cAMP) to creatinine ratio, and undetectab...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405367
更新日期：1984-09-01 00:00:00
abstract：:Glucocorticoid-induced osteoporosis has been reported to be caused by enhanced bone resorption and suppressed bone formation. To clarify whether administration of vitamin K, which enhances bone formation, prevents prednisolone-induced loss of bone mineral density (BMD), a randomized, prospective, controlled study was ...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/pl00005832
更新日期：2000-02-01 00:00:00
abstract：:In this study, we demonstrated that type I collagen matrix induced the expression of osteoblastic phenotypes of bone marrow cells, and that antibone sialoprotein (BSP) monoclonal antibody suppressed the expression of these phenotypes. On the other hand, BSP accelerated the expression of osteoblastic phenotypes of bone...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230010078
更新日期：2000-05-01 00:00:00
abstract：:The effect of the degree of carboxylation of osteocalcin (OC) on the properties of bone is unclear. The aim of this study was to relate serum concentrations of total OC (tOC) and undercarboxylated OC (ucOC), measured with a two-site immunoassay, to bone mineral density (BMD) at the femoral neck and ultrasonic transmit...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s002239900432
更新日期：1998-04-01 00:00:00
abstract：:This study aimed to evaluate the association between objectively measured habitual physical activity and calcaneal and forearm bone mineral density (BMD, g/cm(2)), one mechanically more loaded and one less loaded skeletal region, in children aged 6-8 years. BMD was measured in 297 boys and 265 girls by peripheral dual...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-006-0137-9
更新日期：2007-01-01 00:00:00
