Abstract:
:Inorganic phosphate (P(i)) can regulate the level of skeletal alkaline phosphatase (ALP) activity in human osteoblast-like cells, but not by means of changes in transcription or release from the cell surface. The current studies were intended to determine whether (1) P(i) affected the inactivation of ALP activity in human osteosarcoma (SaOS-2) cells; and (2) P(i)-dependent changes in ALP-specific activity were associated with equal, concomitant changes in the level of ALP immunoreactive protein. The results of these studies revealed that P(i) increased the stability of skeletal ALP activity without equivalent effects on the level of ALP immunoreactive protein. An increase in P(i) (from 0 to 1.8 mmol/liter) caused a time-dependent increase in the amount of skeletal ALP activity in the SaOS-2 cells, without a parallel increase in the amount of skeletal ALP immunoreactive protein, and a decrease in P(i) (from 1.8 to 0 mmol/liter) caused a time-dependent decrease in the amount of ALP activity, without a significant decrease in the total cellular content of ALP immunoreactive protein. Together, these observations suggest that P(i) may alter the level of skeletal ALP activity in SaOS-2 cells by inhibiting a process of irreversible inactivation that does not effect equal, concomitant changes in the level of skeletal ALP immunoreactive protein.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Farley JRdoi
10.1007/BF00302073subject
Has Abstractpub_date
1995-11-01 00:00:00pages
371-8issue
5eissn
0171-967Xissn
1432-0827journal_volume
57pub_type
杂志文章abstract::The absorption kinetics of intranasal salmon calcitonin were assessed in 4 normal subjects and 9 patients with Klinefelter's syndrome. After administration, absorption occurred within 5 minutes. There was a highly significant correlation between the amount administered and the amount absorbed (r = -0.83, P less than 0...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556097
更新日期:1990-02-01 00:00:00
abstract::Osteoclasts are large, multinucleated, terminally differentiated cells formed by the fusion of mononuclear hemopoietic precursors. Their function is the resorption of bone, which is an essential part of the growth, modeling and remodeling of the skeleton. Though some osteoclast differentiation factors have recently be...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-002-2098-y
更新日期:2003-10-01 00:00:00
abstract::Ectopic calcification of diseased tissues or around prosthetic implants can lead to serious disability. Therefore, calcification of implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen was compared with mineralization induced by demineralized bone matrix (DBM). Whereas implants of DBM...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556366
更新日期:1988-05-01 00:00:00
abstract::The bone mineral density (BMD) of the radius and spine was determined by photo absorptiometry in a large number of controls (radius: n = 111; spine: n = 85; age range: 50-79 years) and osteoporotic women (radius: n = 98; spine n = 140; age range: 50-79 years) with at least one "atraumatic" vertebral compression fractu...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554998
更新日期:1990-04-01 00:00:00
abstract::The phorbol ester 12-O-tetradecanoyl-13-acetate (TPA) blocked the growth of, and induced the appearance of processes in the human osteosarcoma cell line U-2 OS. The phorbol ester decreased the intracellular level of alkaline phosphatase (APase) activity (as measured per mg cell protein) and caused a marked increase in...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00582169
更新日期:1992-06-01 00:00:00
abstract::Total fat mass plays a significant role in determining bone mass, but the specific role of central adiposity independent of total fat mass has not been widely studied. Prepubertal (Tanner 1) children (n = 181; 65 boys, 116 girls, 7.8 +/- 1.5 years), including 99 Caucasians and 82 African Americans from Birmingham, Ala...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-006-0116-1
更新日期:2006-12-01 00:00:00
abstract::We compared the cytotoxic effects of alendronate (ALN) and incadronate (YM175) on isolated rabbit osteoclasts in vitro and on rats in vivo. In the in vitro experiment, each bisphosphonate was added to the culture of isolated osteoclasts at the final concentration of 3 x 10(-5), 3 x 10(-4), or 3 x 10(-3) M, and the amo...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900505
更新日期:1998-08-01 00:00:00
abstract::The European Forsteo Observational Study was designed to examine the effectiveness of teriparatide in postmenopausal women with osteoporosis treated for up to 18 months in normal clinical practice in eight European countries. The incidence of clinical vertebral and nonvertebral fragility fractures, back pain, and heal...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-009-9299-6
更新日期:2009-12-01 00:00:00
abstract::To study the mechanism of action of thyroid hormones on bone, we examined the effects of immunosuppressive and nonimmunosuppressive cyclosporins, as well as of transforming growth factor beta 1 (TGF beta 1), 17 beta-estradiol (E2), and dihydroxytestosterone (DHT) on thyroxine (T4)- and triiodothyronine (T3)-stimulated...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298788
更新日期:1992-02-01 00:00:00
abstract::The development of prophylaxes and treatments of bone diseases that can effectively increase the strength of bone as a structure necessitates a better understanding of the time course by which chemical properties define the stiffness of the material during primary and secondary mineralization. It was hypothesized that...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0148-y
更新日期:2005-12-01 00:00:00
abstract::Skeletal tissues contain, apart from cells of the osteogenic and chondrogenic lineage, cells of hemopoietic origin, e.g., macrophages, osteoclasts, and their precursors. In the present study we examined the sensitivity for extracellular ATP4- of the above-mentioned cell types in freshly isolated, bone-derived cell pop...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00297190
更新日期:1994-08-01 00:00:00
abstract::In 11 postmenopausal women with histologically proven osteoporosis and normal lactose tolerance, a direct correlation between calcium absorption and intestinal lactase activity was observed. The results suggest that varying degrees of relative deficiencies in intestinal lactase might contribute to the graded decrease ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02557688
更新日期:1985-01-01 00:00:00
abstract::The native human parathyroid hormone, hPTH-(1-84), and certain carboxyl truncated analogs such as hPTH-(1-34) and even smaller fragments such as hPTH-(1-31)NH2, [Leu27]cyclo(Glu22-Lys26)hPTH-(1-31)NH2, and hPTH-(1-30)NH2 stimulate femoral trabecular and cortical bone growth in ovariectomized (OVX) rats. Here we show t...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010061
更新日期:2000-04-01 00:00:00
abstract::The purpose of the present study was to determine if the type of dietary fat can modify the fatty acid composition and arachidonic acid levels in the alveolar bone phospholipids. Three groups of rats were fed nutritionally adequate semipurified diets containing different lipids: 10% corn oil (control, group I, rich in...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF01351838
更新日期:1993-11-01 00:00:00
abstract::High serum levels of IGFBP-1 are related both to low body mass index (BMI) and to low insulin-like growth factor 1 (IGF-I), which both in turn are related to low bone mineral density (BMD) and to increased fracture risk. However, we have found no previous prospective studies on IGFBP-1 and fracture risk. Despite its n...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-016-0152-4
更新日期:2016-10-01 00:00:00
abstract::Formalin fixation of hard tissues alters the levels of some, but not all, of the parameters routinely measured by bone researchers. Although mineral parameters (ash weight, particle size (beta 1/2 002) and Ca/PO4 ratio) and lipid parameters (total lipid content and composition, extractability, and Ca-acidic phospholip...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02411262
更新日期:1982-07-01 00:00:00
abstract::We describe here the activity of a novel selective estrogen receptor modulator, SP500263. When given to adult ovariectomized (OVX) rats for 28 days at doses of 0.3, 1, or 3 mg/kg/day, we found that SP500263 partially protected against OVX-induced loss of bone mineral content in the distal ends of femurs and in the who...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-002-1029-2
更新日期:2003-06-01 00:00:00
abstract::The present study was designed to characterize dentin formed in transplanted rat molars by investigating calcium (Ca), phosphorus (P), and magnesium (Mg) concentrations using electron probe microanalysis (EPMA) as well as examining the rate of dentin matrix formation by vital staining. The unerupted immature lower rig...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0134-4
更新日期:2006-03-01 00:00:00
abstract::Enamel of intact human teeth laser irradiated in vitro under certain conditions is known to have less subsurface demineralization than unirradiated enamel on exposure to acid; consequently, the potential use of laser irradiance to reduce caries is apparent. The laser-induced physical and/or chemical changes that cause...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/BF02556711
更新日期:1986-04-01 00:00:00
abstract::Anemia is commonly associated with osteoporosis and sarcopenia in older persons. However, there is a common subset of patients identified as osteosarcopenic at a higher risk of adverse outcomes. Whether these patients are also at a higher risk of anemia remains unknown. In this study, we aimed to compare hemoglobin (H...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00706-2
更新日期:2020-08-01 00:00:00
abstract::The effect of parathormone (PTH), lipopolysaccharide (LPS), or interleukin-1 (IL-1) on calcium release and collagen degradation in bone was examined in vitro using labeled neonatal calvaria of normal mice and also of osteopetrotic microphthalmic (mi/mi) mice that have defective osteoclasts. All three agents stimulated...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554878
更新日期:1985-05-01 00:00:00
abstract::Twenty-five female Caucasians, aged 19-57 years, with the hypermobility syndrome had bone density measurements using established noninvasive techniques such as dual X-ray absorptiometry (DXA), single photon absorptiometry (SPA), heel ultrasound (US), and peripheral computed tomography (pQCT) acquisitions of the radius...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223001093
更新日期:2000-07-01 00:00:00
abstract::The effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (2.3 x 10(-12) - 1.4 x 10(-6) [M]) on alkaline phosphatase, collagen, and cell proliferation were examined in primary cultured hypertrophic chondrocytes prepared from the distal epiphyseal growth plate of the tibias of 12-day chick embryos. 1,25(OH)2D3 showed time-...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00296776
更新日期:1992-05-01 00:00:00
abstract::Prosected vertebrae and phalanges from 32 cadavera were examined using noninvasive methods commonly used for assessment of osteoporosis. Bone measures were then compared with a strength variable obtained by mechanically crushing or breaking the bones. All of the phalangeal density assessment techniques were found to b...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555150
更新日期:1988-08-01 00:00:00
abstract::Patients put at bedrest for medical reasons lose 1-2% of spinal bone mineral per week. Losses of this magnitude during even short-term space flights of a few months would pose a serious limitation and require countermeasures. The spinal bone mineral (L2-L4) was determined in 6 healthy males (precision = 2%) before and...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555226
更新日期:1987-11-01 00:00:00
abstract::Bone injury occurs in human hemolytic disorders associated with thrombosis, such as beta-thalassemia and sickle cell disease. Exposure of rats to 2-butoxyethanol (BE) has been associated with hemolytic anemia, disseminated thrombosis, and infarction in multiple organs including bone. This rat model apparently mimics a...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-003-0068-7
更新日期:2004-03-01 00:00:00
abstract::Proton pump inhibitors are taken by millions of patients for prevention and treatment of gastroesophageal diseases. Case-control studies have suggested that use of omeprazole is associated with an increased risk of hip fractures. The aim of this prospective study was to assess the risk of vertebral fractures in postme...
journal_title:Calcified tissue international
pub_type: 杂志文章,多中心研究
doi:10.1007/s00223-008-9188-4
更新日期:2009-01-01 00:00:00
abstract::The existence of nutritional deficiency rickets among infants in sunny Riyadh was confirmed radiologically. Most of the rachitic infants were breast-fed, some received unsupplemented infant feeding formulae, and all live in an environment that is devoid of sunlight. Their mean age at the time of onset was 10.5 months....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02405328
更新日期:1984-05-01 00:00:00
abstract::Aging bone shows a progressive decline in mass and strength. Previous studies have suggested that bone marrow stem cells are reduced with aging and that this could be responsible, in part, for age-associated bone deficits. We measured the number of osteoprogenitor cells present in the bone marrow from adult and aged r...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00296343
更新日期:1995-02-01 00:00:00
abstract::The severest long-term complications of orthopedic joint-prostheses are associated with loosening of the implant-bone interface, due to adverse reactions in bone and subsequent weakening of the connection between the two materials. Mechanical stresses, caused by joint loading, play a key role in the adaption of interf...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02406143
更新日期:1984-01-01 00:00:00