Calcification inhibitors and Wnt signaling proteins are implicated in bovine artery smooth muscle cell calcification in the presence of phosphate and vitamin D sterols.


:Administration of active vitamin D sterols to treat secondary hyperparathyroidism in patients with chronic kidney disease receiving dialysis has been associated with elevated serum calcium and phosphorus levels, which may lead to increased risk of vascular calcification. However, calcimimetics, by binding to the parathyroid gland calcium-sensing receptors, reduce serum parathyroid hormone, calcium, phosphorus, and the calcium-phosphorus product. Using cultured bovine aorta vascular smooth muscle cells (BASMCs), an in vitro model of vascular calcification, we compared calcification levels and gene expression profiles after exposure to the phosphate source ss-glycerolphosphate (BGP), the active vitamin D sterols calcitriol and paricalcitol, the calcimimetic R-568, or BGP with the active vitamin D sterols or R-568. Cells exposed to BGP (10 mM) alone or with calcitriol or paricalcitol showed dose-dependent BASMC calcification. No change in calcification was observed in cultures exposed to BGP with R-568, consistent with the observed lack of calcium-sensing receptor expression. Microarray analysis using total cellular RNA from cultures exposed to vehicle or BGP in the absence and presence of 10(-8) M calcitriol or paricalcitol for 7 days showed that cells exposed to BGP with calcitriol or BGP with paricalcitol had virtually identical gene expression profiles, which differed from those of cells treated with BGP or vehicle alone. Several osteoblast- and chondrocyte-associated genes were modulated by BGP and vitamin D exposure. In this study, exposure of BASMCs to phosphate and active vitamin D sterols induced calcification and changes in expression of genes associated with mineralized tissue.


Calcif Tissue Int


Shalhoub V,Shatzen E,Henley C,Boedigheimer M,McNinch J,Manoukian R,Damore M,Fitzpatrick D,Haas K,Twomey B,Kiaei P,Ward S,Lacey DL,Martin D




Has Abstract


2006-12-01 00:00:00












  • Increased Risk of Hip Fracture in Patients with Lymphoma, a Swedish Population Study of 37,236 Lymphoma Patients.

    abstract::Increased bone loss has been noted in lymphoma patients; however, the incidence of hip fracture is not known. The aim of our study was to explore the risk for hip fracture in patients with lymphoma compared with the entire Swedish population. The risk of hip fracture was determined in a retrospective population cohort...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Johansson P,Lind Kristjansdottir H,Johansson H,Jakir A,Mellström D,Lewerin C

    更新日期:2020-06-01 00:00:00

  • Long-Term Intake of Green Tea Extract Causes Mal-Conformation of Trabecular Bone Microarchitecture in Growing Rats.

    abstract::The purpose of this study was to examine the effects of green tea extract (GTE) intake on bone structural and physiological properties, such as bone mass, trabecular bone microarchitecture, cortical bone geometry, and bone mechanical strength, in growing rats. Four-week-old male Wistar rats were divided into the follo...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Minematsu A,Nishii Y,Imagita H,Sakata S

    更新日期:2018-03-01 00:00:00

  • In vitro synthesis of type I collagen: quantification of carboxyterminal propeptide of procollagen type I versus tritiated proline incorporation.

    abstract::Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen has been recently introduced to measure in vitro synthesis of type I collagen by osteoblasts and fibroblasts. However, it has not been assessed whether the equivalent results are obtained with this new assay and with tritiated proline incor...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Nacher M,Serrano S,Mariñoso ML,García MC,Bosch J,Díez A,Lloreta J,Aubia J

    更新日期:1999-03-01 00:00:00

  • Changes in heated and in laser-irradiated human tooth enamel and their probable effects on solubility.

    abstract::Enamel of intact human teeth laser irradiated in vitro under certain conditions is known to have less subsurface demineralization than unirradiated enamel on exposure to acid; consequently, the potential use of laser irradiance to reduce caries is apparent. The laser-induced physical and/or chemical changes that cause...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审


    authors: Fowler BO,Kuroda S

    更新日期:1986-04-01 00:00:00

  • Size and shape of mineralites in young bovine bone measured by atomic force microscopy.

    abstract::Atomic force microscopy (AFM) was used to obtain three-dimensional images of isolated mineralites extracted from young postnatal bovine bone. The mean mineralite size is 9 nm x 6 nm x 2.0 nm, significantly shorter and thicker than the mineralites of mature bovine bone measured by the same technique. Mineralites of the...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Tong W,Glimcher MJ,Katz JL,Kuhn L,Eppell SJ

    更新日期:2003-05-01 00:00:00

  • Modulation of matrix vesicle enzyme activity and phosphatidylserine content by ceramic implant materials during endosteal bone healing.

    abstract::This study examined effects of bone bonding and nonbonding implants on parameters associated with matrix vesicle-mediated primary bone formation, matrix vesicle alkaline phosphatase and phospholipase A2 specific activities, and phosphatidylserine content. Tibia marrow ablation followed by implantation of KG-Cera, Mina...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Schwartz Z,Swain LD,Marshall T,Sela J,Gross U,Amir D,Muller-Mai C,Boyan BD

    更新日期:1992-12-01 00:00:00

  • Bone turnover markers and PTH levels in surgical versus natural menopause.

    abstract::In order to assess similarities and differences in women that suffer surgical versus natural menopause, a series of bone, clinical, and biochemical parameters was assayed in a clinical sample of 35 women with surgical menopause and 112 women with natural menopause. Biochemical parameters included hormones [parathyroid...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: García-Pérez MA,Moreno-Mercer J,Tarín JJ,Cano A

    更新日期:2004-02-01 00:00:00

  • Serum levels of bone Gla protein (osteocalcin, BGP) and carboxyterminal propeptide of type I procollagen (PICP) in acromegaly: effects of long-term octreotide treatment.

    abstract::We measured serum concentrations of bone Gla-protein (osteocalcin, BGP) and carboxyterminal propeptide of type I procollagen (PICP) in 14 patients with active acromegaly. Blood was collected at 0800 for measurement of bone Gla-protein (BGP), carboxyterminal propeptide of type I procollagen (PICP), and insulin-like gro...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Terzolo M,Piovesan A,Osella G,Pia A,Reimondo G,Pozzi C,Raucci C,Torta M,Paccotti P,Angeli A

    更新日期:1993-03-01 00:00:00

  • The beneficial effect of leisure-time physical activity on bone mineral density in pre- and postmenopausal women.

    abstract::Regular exercise and physical activity (PA) are known to be protective factors for maintaining bone mineral density (BMD) and preventing osteoporotic fracture. We investigated the associations between leisure-time PA and BMD in 2,903 premenopausal and 2,267 postmenopausal women in Korea. BMDs of the lumbar spine and f...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Kim KZ,Shin A,Lee J,Myung SK,Kim J

    更新日期:2012-09-01 00:00:00

  • Associations Between Objectively Measured Physical Activity, Body Composition and Sarcopenia: Findings from the Hertfordshire Sarcopenia Study (HSS).

    abstract::Regular physical activity (PA) is associated with reduced risk of the development and progression of musculoskeletal, metabolic and vascular disease. However, PA declines with age and this can contribute to multiple adverse outcomes. The aims of this study were to describe the relationship between accelerometer-determ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Westbury LD,Dodds RM,Syddall HE,Baczynska AM,Shaw SC,Dennison EM,Roberts HC,Sayer AA,Cooper C,Patel HP

    更新日期:2018-09-01 00:00:00

  • Transplanted chondrocytes inhibit endochondral ossification within cartilage repair tissue.

    abstract::The aim of this study was to investigate the effect of transplanted chondrocytes on endochondral bone formation in cartilage repair tissue. In the knee joint of miniature pigs, cartilage lesions were treated by microfracturing and were then either left empty, covered with a collagen membrane, or treated by matrix-asso...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Blanke M,Carl HD,Klinger P,Swoboda B,Hennig F,Gelse K

    更新日期:2009-11-01 00:00:00

  • Impact of Conventional Medical Therapy on Bone Mineral Density and Bone Turnover in Adult Patients with X-Linked Hypophosphatemia: A 6-Year Prospective Cohort Study.

    abstract::X-linked hypophosphatemia (XLH) is a rare, inheritable disorder manifesting as rickets in children and osteomalacia in adults. While conventional medical treatment with oral phosphate and alfacalcidol is recommended in childhood, it is undecided whether adults should continue therapy. The aim of this 6-year prospectiv...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Shanbhogue VV,Hansen S,Jørgensen NR,Beck-Nielsen SS

    更新日期:2018-03-01 00:00:00

  • The enamel fluid in the early secretory stage of porcine amelogenesis: chemical composition and saturation with respect to enamel mineral.

    abstract::The present study reports on the separation of fluid from soft, "cheeselike" enamel of porcine permanent teeth in the secretory stage, and the determination of its chemical composition. The enamel tissues were dissected from mandibles of 5 to 6-month-old piglets and pooled under mineral oil in centrifuge tubes, and th...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Aoba T,Moreno EC

    更新日期:1987-08-01 00:00:00

  • Skeletal demineralization in Turner's syndrome.

    abstract::The bone mineral status of 17 girls with Turner's syndrome was evaluated by single photon absorptiometry. Bone mineral content (BMC) was 25.4% below that predicted by normalization for age, sex, height, weight, and bone width. Only 25% of this demineralization could be attributed to delayed skeletal maturation. Bones ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Shore RM,Chesney RW,Mazess RB,Rose PG,Bargman GJ

    更新日期:1982-01-01 00:00:00

  • Dkk1 KO Mice Treated with Sclerostin Antibody Have Additional Increases in Bone Volume.

    abstract::Dickkopf-1 (DKK1) and sclerostin are antagonists of the Wnt/β-catenin pathway and decreased expression of either results in increased bone formation and mass. As both affect the same signaling pathway, we aimed to elucidate the redundancy and/or compensation of sclerostin and DKK1. Weekly sclerostin antibody (Scl-Ab) ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Morse A,Cheng TL,Schindeler A,McDonald MM,Mohanty ST,Kneissel M,Kramer I,Little DG

    更新日期:2018-09-01 00:00:00

  • Treatment thresholds for osteoporosis and reimbursability criteria: perspectives associated with fracture risk-assessment tools.

    abstract::The definition of osteoporosis was based for several years on bone mineral density values, which were used by most guidelines for defining treatment thresholds. The availability of tools for the estimation of fracture risk, such as FRAX™ or its adapted Italian version, DeFRA, is providing a way to grade osteoporosis s...

    journal_title:Calcified tissue international

    pub_type: 社论


    authors: Adami S,Bertoldo F,Gatti D,Minisola G,Rossini M,Sinigaglia L,Varenna M

    更新日期:2013-09-01 00:00:00

  • Combined Biomaterials: Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model.

    abstract::Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane wa...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Dziedzic DSM,Francisco JC,Mogharbel BF,Irioda AC,Stricker PEF,Floriano J,de Noronha L,Abdelwahid E,Franco CRC,de Carvalho KAT

    更新日期:2021-01-09 00:00:00

  • Hip fracture in nursing homes: an Italian study on prevalence, latency, risk factors, and impact on mobility.

    abstract::Hip fracture may cause and/or complicate institutionalization. We undertook this study to define its overall prevalence among the residents of four nursing homes in Central Italy as well as its latency and impact on mobility when it occurred within institutions. We also performed a case control analysis with the aim o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Maggio D,Ubaldi E,Simonelli G,Cenci S,Pedone C,Cherubini A

    更新日期:2001-06-01 00:00:00

  • The relationship between serum intact parathyroid hormone and calcium in idiopathic hypoparathyroidism.

    abstract::We studied the relationship between serum calcium (Ca) and parathyroid hormone (PTH) in patients with idiopathic hypoparathyroidism (IHP) to investigate the diversity of the degree of parathyroid insufficiency and a regulation of PTH secretion by Ca in IHP. Serum intact PTH concentrations ranged from < 1.0 to 10.3 pg/...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Mizunashi K,Furukawa Y,Abe K,Yoshinaga K

    更新日期:1993-12-01 00:00:00

  • Menopausal bone loss is partially regulated by dietary intake of vitamin D.

    abstract::Five years ago we reported results from a cross-sectional study of the effect of nutritional factors on calcium-regulating hormones and bone loss in perimenopausal women. We found an inverse correlation between serum 25-hydroxyvitamin D (25OHD) and immunoreactive parathyroid hormone (PTH), and we postulated that over ...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章


    authors: Lukert B,Higgins J,Stoskopf M

    更新日期:1992-09-01 00:00:00

  • The amelogenin problem: a comparison of purified enamel matrix proteins.

    abstract::Using a combination of gel filtration and DEAE-cellulose chromatography, together with small-scale preparative polyacrylamide gel electrophoresis, we isolated five proteins (amelogenins) from demineralized bovine fetal dental enamel matrix. These purified proteins were characterized by amino acid analysis and gel elec...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Fincham AG

    更新日期:1979-03-13 00:00:00

  • The matrix of endochondral bone differs from the matrix of intramembranous bone.

    abstract::Osseous tissue develops via two distinctly different processes: endochondral (EC) ossification and intramembranous (IM) ossification. The present study tests the hypothesis that each type of osseous tissue contains unique inducing factors for the promotion of cartilage and bone development. Previous work suggests that...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Scott CK,Hightower JA

    更新日期:1991-11-01 00:00:00

  • Cancellous bone of the spine is greater in black women.

    abstract::The bone mineral density (BMD) of the spine was measured in the posteroanterior (PA) and lateral projections as well as the total body BMD in 447 black and white women. The lateral projection is comprised predominantly of cancellous bone whereas the total body BMD is predominantly cortical bone, and the PA spine is in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Aloia JF,Vaswani A,Mikhail M,Badshah M,Flaster E

    更新日期:1999-07-01 00:00:00

  • Fracture risk associated with different types of oral corticosteroids and effect of termination of corticosteroids on the risk of fractures.

    abstract::We conducted a case-control study on fracture risk associated with the use of orally administered prednisolone/prednisone, budesonide, methylprednisolone, and hydrocortisone to assess if the various preparations were associated with different fracture patterns. Cases were all subjects with any fracture sustained durin...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Vestergaard P,Rejnmark L,Mosekilde L

    更新日期:2008-04-01 00:00:00

  • Effect of ascorbic acid on protein synthesis and collagen hydroxylation in continuous flow organ cultures of adult mouse periodontal tissues.

    abstract::A continuous flow organ culture system (CFCS) was used to determine the effect of ascorbic acid on the synthesis of collagen and noncollagenous protein by bone of the alveolar process and periodontal ligament in organ cultures of adult mouse periodontium. For the last 24 h of 2 day cultures, 5 microCi/ml 3H-proline wa...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Sodek J,Feng J,Yen EH,Melcher AH

    更新日期:1982-07-01 00:00:00

  • Serum Sclerostin and Bone Morphogenetic Protein-2 Levels in Patients with Ankylosing Spondylitis: A Meta-Analysis.

    abstract::Various studies have investigated the serum sclerostin and bone morphogenetic protein-2 (BMP-2) levels in patients with ankylosing spondylitis (AS), but the results were inconsistent. The aim of this meta-analysis was to synthetically assess the associations of serum levels of sclerostin and BMP-2 with AS. Multiple el...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,meta分析


    authors: Yang J,Xu S,Chen M,Yuan Y,Zhang X,Ma Y,Wu M,Han R,Hu X,Liu R,Deng J,Guan S,Gao X,Pan M,Xu S,Shuai Z,Jiang S,Guan S,Chen L,Pan F

    更新日期:2019-07-01 00:00:00

  • Neridronate preferentially suppresses the urinary excretion of peptide-bound deoxypyridinoline in postmenopausal women.

    abstract::ELISAs for measuring the urinary excretion of collagen crosslinks and related peptides appear to show marked differences in sensitivity to anti-resorptive therapy. This presumably reflects variations in specificity of the anylate being detected in these assays, and the way in which they respond to treatment. To clarif...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Tobias JH,Laversuch CV,Wilson N,Robins SP

    更新日期:1996-11-01 00:00:00

  • Ultrasound and dual X-ray absorptiometry densitometry in women with hip fracture.

    abstract::To assess the usefulness of the measurement of the os calcis by ultrasound, a method that probably reflects bone quality as well as density, we have studied 54 women with hip fracture of the proximal femur and a control group. Ultrasound evaluation of the os calcis [broadband ultrasound attenuation (BUA), speed of the...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Mautalen C,Vega E,González D,Carrilero P,Otaño A,Silberman F

    更新日期:1995-09-01 00:00:00

  • Influence of Teriparatide and Ibandronate on Cortical Bone in New Zealand White Rabbits: A HR-QCT Study.

    abstract::Teriparatide (TPTD) is known to increase the cortical thickness and porosity. The purpose of the present study was to determine whether switching from TPTD to ibandronate (IBN) would be useful for improving cortical bone parameters as assessed using high-resolution quantitative computed tomography (HR-QCT) analyses in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Iwamoto J,Seki A,Nango N

    更新日期:2016-11-01 00:00:00

  • A new theory of bone lamellation.

    abstract::A comparative polarized light (PLM), scanning (SEM), and transmission (TEM) electron microscopy study was carried out on cross- and longitudinal sections of human lamellar bone in the tibiae of four male subjects aged 9, 23, 45, and 70 years. SEM analysis was also performed on rectangular-prismatic samples in order to...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Marotti G

    更新日期:1993-01-01 00:00:00