Abstract:
:Mature dental enamel is the most mineralized of all mammalian tissues and considered to be free of collagen. Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are two nonreducible cross-links of mature collagen. Hydroxyproline (Hyp) is an amino acid that is believed to be indicative of the presence of collagen. We set out to assess the concentrations of Hyp, HP, and LP in dental enamel and dentin (control) to clarify whether there was minor collagen content in dental enamel. We studied 17.53 g of enamel and 22.12 g of dentin gained from 120 extracted human teeth. Enamel and dentin (control) were separated with a diamond dental drill under microscopic control by wasting a margin of enamel (Ca. 2 mm) at the dentin-enamel border. Collagen alpha-chains were analyzed by Sodium dodecylsulfate-polyacrylamide gel (SDS-PAGE) after decalcification and collagen extraction. Concentrations of HP and LP where measured by using high-performance liquid chromatography (HPLC). Hyp was analyzed by a spectrophotometric method. The pooled probe of enamel contained 0.23 mug/g of Hyp. This concentration was 49 times lower than that in dentin. Concentrations of HP and LP in enamel were 0.07 nmol/g and 0.02 nmol/g, respectively being 605.57 (HP) and 251.50 (LP) times lower in enamel as compared to dentin. Collagen type I was found in enamel; collagen types I and V were found in dentin samples. In reports of many studies and textbooks, collagen is considered to be completely absorbed in the course of the mineralization and maturation of dental enamel. We show that this is not the case. However, the concentration of collagen in enamel was considerably lower as compared to that in dentin.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Açil Y,Mobasseri AE,Warnke PH,Terheyden H,Wiltfang J,Springer Idoi
10.1007/s00223-004-0122-0subject
Has Abstractpub_date
2005-02-01 00:00:00pages
121-6issue
2eissn
0171-967Xissn
1432-0827journal_volume
76pub_type
杂志文章abstract::The aim of this study was to investigate the relation between birth weight and calcaneal bone stiffness in a large sample of Belgian, healthy, preadolescent children. Participants were 827 children (3.6-11.2 years, 51.6 % boys) from the Belgian cohort of the IDEFICS study. Birth weight was obtained using a parental qu...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-012-9636-z
更新日期:2012-10-01 00:00:00
abstract::Undesired bone loss around implants is considered to occur mainly because of a stress-shielding phenomenon. Bone surrounding the total knee arthroplasty (TKA) adjusts its mineral density and structure to meet new mechanical demands. Immobilization, in combination with local operative trauma to the bone and soft tissue...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00223-002-1022-9
更新日期:2002-12-01 00:00:00
abstract::The purpose of the present work was to investigate the appearance of ossification centers in human femur by Dual-energy X-ray Absorptiometry (DXA), comparing densitometric data with morphogenetic events. Posteroanterior scans were performed on 31 dried femora (from 11.5 weeks of conceptual age to 1 year of postnatal l...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/pl00005841
更新日期:2000-04-01 00:00:00
abstract::Osteocalcin, the gamma-carboxyglutamic acid-containing protein, which is the most abundant noncollagenous protein of bone and dentin, is considered to play roles in bone formation and remodeling. It is unclear how the gamma-carboxyglutamic acid side-chains in osteocalcin coordinate to Ca2+, since the X-ray structure o...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010042
更新日期:2001-12-01 00:00:00
abstract::We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Gr...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-003-0110-9
更新日期:2004-03-01 00:00:00
abstract::The effect of SrCl(2) treatment on bone nanostructure in a rat ovariectomy model was studied using scanning small-angle X-ray scattering (sSAXS). Twelve 6-month-old female Wistar rats were used. Six animals were ovariectomized (+ovx) and six were left intact after sham surgery (-ovx). Six animals, three +ovx and three...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-010-9341-8
更新日期:2010-04-01 00:00:00
abstract::There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900111
更新日期:1996-09-01 00:00:00
abstract::Humanin (HN), a mitochondrial derived peptide, plays cyto-protective role under various stress. In this study, we aimed to investigate the effects of HNGF6A, an analogue of HN, on osteoblast apoptosis and differentiation and the underlying mechanisms. Cell proliferation of murine osteoblastic cell line MC3TC-E1 was ex...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00660-z
更新日期:2020-05-01 00:00:00
abstract::Tartrate resistant acid phosphatase (TRAP) has been proposed as a new biochemical marker for bone resorption. We have compared this new marker, TRAP, with the classical biochemical markers of bone remodelling, serum alkaline phosphatase (sAP), serum osteocalcin (sBGP), and with the urinary hydroxyproline/creatinine ra...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555896
更新日期:1991-07-01 00:00:00
abstract::Primary cilia are responsible for sensing mechanical loading in osteocytes. However, the underlying working mechanism of cilia remains elusive. An osteocyte model is necessary to reveal the role of cilia. Furthermore, the osteocyte model should be with upregulated or downregulated primary cilium expression. Herein, we...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00756-6
更新日期:2020-12-01 00:00:00
abstract::The effects of fluoride on human enamel and selachian enameloid in vitro were visualized in TEM and analyzed with electron diffraction. It is demonstrated that under precise pH conditions, inducing concentration balance between F-ions and apatite, calcium fluoride is no longer formed, and crystalline changes occur ins...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02409406
更新日期:1981-01-01 00:00:00
abstract::Several rare inherited disorders have been described that show phenotypic overlap with Paget's disease of bone (PDB) and in which PDB is a component of a multisystem disorder affecting muscle and the central nervous system. These conditions are the subject of this review article. Insertion mutations within exon 1 of t...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-019-00520-5
更新日期:2019-05-01 00:00:00
abstract::Our knowledge of the developmental changes in the concentration of serum and bone osteocalcin (OC) is limited. To investigate the interrelationship between skeletal and circulatory OC during acquisition of peak bone density in mice, we examined the temporal changes in the concentration of serum and bone OC from 3 to 1...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-1106-y
更新日期:2002-11-01 00:00:00
abstract::Age-related loss of skeletal muscle is associated with increased risk of functional limitation and cardiovascular (CV) mortality. In the elderly abdominal aortic calcification (AAC) can increase CV risk by altering aortic properties which may raise blood pressure and increase cardiac workload. This study investigated ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-016-0157-z
更新日期:2016-10-01 00:00:00
abstract::Pleiotrophin (PTN) was found to have potent effects on regulation of osteoblast recruitment, proliferation and differentiation. The present study examined the long-term effects of targeted PTN over-expression on bone development and repair in a transgenic mouse model. Femurs and tibiae from the PTN transgenic mice and...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-004-0145-6
更新日期:2005-04-01 00:00:00
abstract::Allogenous demineralized bone matrix (DBM) represents a potential scaffold for bone tissue engineering due to its close relation in structure and function with autologous bone, but its supply is often restricted by donor availability. Thus, an expanded source of human bone is needed. The aim of this study was to evalu...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-008-9159-9
更新日期:2008-09-01 00:00:00
abstract::This study examined bone density among postmenopausal Buddhist nuns and female religious followers of Buddhism in southern Taiwan and related the measurements to subjects characteristics including age, body mass, physical activity, nutrient intake, and vegetarian practice. A total of 258 postmenopausal Taiwanese veget...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/pl00005812
更新日期:1997-03-01 00:00:00
abstract::This study revealed the distinguished changes of preferential orientation of collagen and apatite and Young's modulus in two different types of osteoporotic bones compared with the normal bone. Little is known about the bone material properties of osteoporotic bones; therefore, we aimed to assess material properties i...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-018-0508-z
更新日期:2019-04-01 00:00:00
abstract::Irrespective of the method used for noninvasive bone mass determination, data comparison between different centers is a major problem as significant interunit variation may occur. We, therefore, have employed mobile densitometry units to reduce interunit variability in two large epidemiologic studies in Germany. Two c...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00301682
更新日期:1994-03-01 00:00:00
abstract::Familial hypocalciuric hypercalcemia (FHH) is often considered in the differential diagnosis of hyperparathyroidism, but is rarely diagnosed. So far, FHH has not been documented in Israel. This report presents preliminary evidence for the occurrence of FHH in Israel. ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900290
更新日期:1997-07-01 00:00:00
abstract::Osteoporosis is commonly associated with estrogen deficiency. However, the mechanisms by which the lack of this hormone causes bone loss are poorly understood. The bone structure of the oral cavity seems to be affected by estrogen deficiency, since a delayed healing process after tooth extraction has been observed aft...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-004-0013-4
更新日期:2005-02-01 00:00:00
abstract::This article reviews the effects of restrictive eating disorders on bone health. The relationship between eating disorders and amenorrhea is discussed in detail. The pathologic impact of malnutrition on bone is explored by examining the results of studies using various available imaging techniques. The multiple hormon...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-016-0164-0
更新日期:2017-05-01 00:00:00
abstract::In 11 postmenopausal women with histologically proven osteoporosis and normal lactose tolerance, a direct correlation between calcium absorption and intestinal lactase activity was observed. The results suggest that varying degrees of relative deficiencies in intestinal lactase might contribute to the graded decrease ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02557688
更新日期:1985-01-01 00:00:00
abstract::This study examines the efficacy of using radiolabeled thymidine as a measure of bone cell DNA synthesis. The effect of a bone-active peptide on cell proliferation is assessed under different labeling conditions and it is shown that the apparent stimulation in DNA synthesis is due to an increase in participating cells...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02553298
更新日期:1986-08-01 00:00:00
abstract::To investigate the effect of low doses of intranasal salmon calcitonin on plasma cyclic AMP (cAMP) and serum ionized calcium, 40 healthy postmenopausal women were randomized to receive a single dose of either placebo or 50, 100, or 200 IU of salmon calcitonin as a nasal spray. Blood samples were collected throughout a...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF02555817
更新日期:1990-01-01 00:00:00
abstract::This randomized, double-blind study assessed the antifracture efficacy and safety of intermittent intravenous (IV) ibandronate versus oral daily risedronate in Japanese patients with primary osteoporosis. Ambulatory patients aged ≥60 years were randomized to receive 0.5 or 1 mg/month IV ibandronate plus oral daily pla...
journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00223-013-9734-6
更新日期:2013-08-01 00:00:00
abstract::The effect of ipriflavone (IP), a synthetic isoflavonoid derivative, on in vivo bone formation was studied in rat perialveolar bone by surgically producing a hole in the mandibular bone. The holes were filled either with powdered IP or with compounds containing no osteoinductive properties such as biostite and Htr (ha...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900533
更新日期:1998-10-01 00:00:00
abstract::Results from previous quasi-static mechanical tests indicate that femurs from elderly subjects fail in vitro at forces 50% below those available in a fall from standing height. However, bone is a rate-dependent material, and it is not known whether this imbalance is present at rates of loading which occur in a fall. B...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
doi:10.1007/BF00310169
更新日期:1994-07-01 00:00:00
abstract::The effect of the degree of carboxylation of osteocalcin (OC) on the properties of bone is unclear. The aim of this study was to relate serum concentrations of total OC (tOC) and undercarboxylated OC (ucOC), measured with a two-site immunoassay, to bone mineral density (BMD) at the femoral neck and ultrasonic transmit...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s002239900432
更新日期:1998-04-01 00:00:00
abstract::Plasma osteocalcin (BGP), growth hormone (GH), and somatomedin C(SmC) were measured in 12 patients with acromegaly [7 clinically active (aA), 5 cured (cA)] and 9 control subjects (C). Basal plasma values for the three parameters were higher in aA than in C and in cA. No significant difference was found between cA and ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555167
更新日期:1988-07-01 00:00:00