Abstract：
:Radiation therapy (RT) is an established treatment modality for malignant neoplasms. RT induces tissue damage that may lead to osteoradionecrosis in more severe cases. Suitable animal models to study RT-induced changes in membranous craniofacial bone are currently not available. The aim of this study was therefore to quantify RT-induced changes in cranial microcirculation using a newly developed calvaria chamber model and to relate these changes to RT-induced histological damage. New Zealand white rabbits received a total radiation dose of 18.75 Gy through the calvaria chamber, and the number of vessels, the vessel length density (VLD), and angiogenic sprouting were quantified on a weekly basis during a 12-week period. At the end of 12 weeks, the RT-treated (n = 5) or control (n = 5) calvarias were biopsied for histopathological analysis. RT resulted in a steep reduction in the number of vessels and the VLD during the first 3 weeks, particularly in larger-diameter vessels, followed by a flat stabilization/remodeling phase in the subsequent 9 weeks that never restored to baseline values. Histomorphometric analysis revealed a high degree of osteocytic depletion, prominent hypocellularity in the lacunae and intraosseous vasculature, enlarged and nonconcentric Haversian systems, and a severely disorganized bone matrix in the RT-treated calvarias. Despite the prevalence of some angiogenic potential, the RT-induced effects in the early phase persisted in the intermediate to late phase, which may have contributed to the poor recovery of the RT-treated bone.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Desmons S,Heger M,Delfosse C,Falgayrac G,Sarrazin T,Delattre C,Catros S,Mordon S,Penel Gdoi
10.1007/s00223-009-9217-ysubject
Has Abstractpub_date
2009-05-01 00:00:00pages
379-87issue
5eissn
0171-967Xissn
1432-0827journal_volume
84pub_type
杂志文章abstract：:Targeted disruption of the c-src gene leads to a severe form of osteopetrosis in mice [2]. As the c-src gene is expressed in all tissues and cells tested, we have analyzed fibroblasts from three individuals with malignant, congenital osteopetrosis for the expression of c-src at the protein level. No differences could ...
journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 评论,杂志文章,评审
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
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