Abstract：
:Amelogenins, the major protein components of the enamel extracellular matrix, are postulated to be involved in controlling the elongated and oriented growth of enamel carbonated apatite crystals. In order to clarify the functional role of amelogenin during the early stage of enamel biomineralization, octacalcium phosphate (OCP) crystals, known to be potent precursors of hydroxyapatite, were grown in 1-10% (w/w) native bovine and two recombinant murine amelogenins. Amelogenins were solution-like at 1% and formed gel at 10%, while 5% amelogenins became gel after reaction and it was inhomogeneous and porous. Morphological changes of OCP crystals were evaluated as the function of amelogenin concentration by analyzing the mean values of length, width, thickness, their reduction ratios (L/Lc, W/Wc, T/Tc) as well as L/W and W/T ratios. Length, width, and thickness decreased in a does-dependent manner. Length decreased almost linearly in 1%-10%, whereas width decreased drastically in 1%-5% while the decrease from 5% to 10% was small. As a result, elongated morphology of OCP crystal was most emphasized in 5% bovine amelogenins and rM166 and 2%-5% rM179. The size reduction was in the order of W/Wc < L/Lc < T/Tc. We therefore concluded that amelogenin interaction with crystal faces was in the order (010) > (001) > (100). At all concentrations, W/ Wc was significantly the smallest. This indicated that the primary role of amelogenin was to decrease the width of OCP by blocking the hydrophobic (010) faces. We suggest that the drastic decrease of crystal width is the result of interaction of the densely packed nanospheres in 5%-10% amelogenin.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Iijima M,Moradian-Oldak Jdoi
10.1007/s00223-002-0011-3subject
Has Abstractpub_date
2004-06-01 00:00:00pages
522-31issue
6eissn
0171-967Xissn
1432-0827journal_volume
74pub_type
杂志文章abstract：:The fossil record shows that bone remodeling has existed since the earliest large vertebrates became weight-bearing on land, but the functions of remodeling have long been debated. The principal protagonists in this debate have been those favoring a mechanical function and those asserting that remodeling serves to mov...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-002-1059-9
更新日期：2003-08-01 00:00:00
abstract：:The recent advancement of high resolution magnetic resonance imaging has opened up new avenues for the determination of structural characteristics of the trabecular network, which may significantly improve the diagnosis of osteoporosis. An analysis of the calcaneus in healthy women has shown similar age-related change...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230001168
更新日期：2001-03-01 00:00:00
abstract：:Ultrasonic methods measure properties intrinsic to bone's structure that are not necessarily dependent on bone mass. Therefore, ultrasound may prove to be a useful tool for diagnosing bone fragility and osteoporosis. The goal of this study was to determine the relationship between apparent velocity of ultrasound (AVU)...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02565132
更新日期：1991-08-01 00:00:00
abstract：:Radiation therapy (RT) is an established treatment modality for malignant neoplasms. RT induces tissue damage that may lead to osteoradionecrosis in more severe cases. Suitable animal models to study RT-induced changes in membranous craniofacial bone are currently not available. The aim of this study was therefore to ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9217-y
更新日期：2009-05-01 00:00:00
abstract：:Calcium and phosphate were allowed to diffuse into gelatin and Type I collagen gels which were then cut into slices and analyzed for ion concentrations. Solutions of calcium and phosphate were then prepared, with ion concentrations equivalent to the highest levels in the slices, and mixed together, whereupon a rapid a...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556459
更新日期：1991-06-01 00:00:00
abstract：:Dickkopf-1 (DKK1) and sclerostin are antagonists of the Wnt/β-catenin pathway and decreased expression of either results in increased bone formation and mass. As both affect the same signaling pathway, we aimed to elucidate the redundancy and/or compensation of sclerostin and DKK1. Weekly sclerostin antibody (Scl-Ab) ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0420-6
更新日期：2018-09-01 00:00:00
abstract：:The purpose of this study was to investigate the mineral induction capacity in vitro of polyanionic proteins covalently bound to a surface. Rat dentin gamma-carboxyglutamate-containing protein of the osteocalcin type (Gla-protein), proteoglycan (PG), and phosphoprotein (PP-H), as well as phosvitin (PhV) and bovine ser...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02553763
更新日期：1989-04-01 00:00:00
abstract：:During endochondral ossification, proliferative activity of chondrocytes is arrested and the cells undergo terminal hypertrophic differentiation. We examined the expression of the cyclin-dependent kinase inhibitor, p21(CIP1/WAF1) in permanent cartilage (xyphoid and articular cartilage) and in cartilage undergoing endo...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900323
更新日期：1997-09-01 00:00:00
abstract：:Further insight into human tooth enamel, dense fraction (TE), has been obtained by following the change and loss of CO3(2-), OH-, structurally incorporated H2O, Cl-, and, indirectly, HPO4(2-) after TE had been heated in N2 or vacuum in the range 25-1000 degrees C. Quantitative infrared spectroscopic, lattice parameter...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02407181
更新日期：1980-01-01 00:00:00
abstract：:Prosected vertebrae and phalanges from 32 cadavera were examined using noninvasive methods commonly used for assessment of osteoporosis. Bone measures were then compared with a strength variable obtained by mechanically crushing or breaking the bones. All of the phalangeal density assessment techniques were found to b...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555150
更新日期：1988-08-01 00:00:00
abstract：:Human osteoblasts were obtained by migration and proliferation of cells from embryonic membranous bone on glass fragments. Light and electron microscopy analyses revealed a typical osteoblast-like appearance with high protein synthesis activity. The cells showed high alkaline phosphatase activity that was associated w...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00316880
更新日期：1992-11-01 00:00:00
abstract：:Aluminum ions (Al) and chromium (III) ions (Cr), as they exist in aqueous solution at neutral pH, adsorb onto calcium hydroxyapatite crystals (HAP) and severely inhibit their dissolution process, when present in concentrations less than 1 microM. Iron (III) ions (Fe), at concentrations up to 10 microM, have no effect ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555127
更新日期：1987-07-01 00:00:00
abstract：:Regular physical activity (PA) is associated with reduced risk of the development and progression of musculoskeletal, metabolic and vascular disease. However, PA declines with age and this can contribute to multiple adverse outcomes. The aims of this study were to describe the relationship between accelerometer-determ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0413-5
更新日期：2018-09-01 00:00:00
abstract：:An organ culture system has been developed whereby mechanical stress can be applied to cranial sutures under controlled experimental conditions. The application of a continuous tensile mechanical stress (30 g) to cranial sutures from newborn rabbits (1--2 days) was accompanied by a significant increase in the incorpor...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02441232
更新日期：1979-10-31 00:00:00
abstract：:Inorganic phosphate (P(i)) can regulate the level of skeletal alkaline phosphatase (ALP) activity in human osteoblast-like cells, but not by means of changes in transcription or release from the cell surface. The current studies were intended to determine whether (1) P(i) affected the inactivation of ALP activity in h...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00302073
更新日期：1995-11-01 00:00:00
abstract：:Little is known about hypoxia-induced modification of the canal network in the cortical bone despite its involvement in intracortical vascularity and bone blood supply. In this study, we examined the effect of chronic hypoxia on the canal network in postnatal bone. Tibiae were harvested from 4- and 8-week-old rats (hy...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9415-7
更新日期：2011-01-01 00:00:00
abstract：:The effect of zinc on in vitro deoxyribonucleic acid (DNA) synthesis activity in the femoral-diaphyseal and metaphyseal tissues of newborn rats was investigated to determine a role of zinc in bone growth. In vitro DNA synthesis was assayed in a reaction mixture containing the 100 g centrifugation supernatant, which in...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-2010-1
更新日期：2001-09-01 00:00:00
abstract：:Teriparatide (TPTD) is the most widely used anabolic agent in the treatment of patients with osteoporosis although its use is restricted in many countries. A recent randomised trial confirmed that TPTD was superior to risedronate at preventing vertebral fractures over a 2-year period. There is limited information on t...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00563-8
更新日期：2019-08-01 00:00:00
abstract：:The absorption kinetics of intranasal salmon calcitonin were assessed in 4 normal subjects and 9 patients with Klinefelter's syndrome. After administration, absorption occurred within 5 minutes. There was a highly significant correlation between the amount administered and the amount absorbed (r = -0.83, P less than 0...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556097
更新日期：1990-02-01 00:00:00
abstract：:Studies suggest that long-term use of proton pump inhibitors (PPIs) may be associated with an increased risk of fracture. However, the role of medication adherence in this association is not fully understood. A retrospective cohort study was conducted to examine the relationship between PPI use/adherence and fracture ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-014-9855-6
更新日期：2014-06-01 00:00:00
abstract：:Impact activity is an important contributor to bone accrual. Children engaging in such activity have been shown to have greater bone mineral density (BMD) than their peers. This cross-sectional study was designed to quantify the association between BMD and impact activity in pre-pubescent girls, specifically examining...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-1131-x
更新日期：2003-01-01 00:00:00
abstract：:Osseous tissue develops via two distinctly different processes: endochondral (EC) ossification and intramembranous (IM) ossification. The present study tests the hypothesis that each type of osseous tissue contains unique inducing factors for the promotion of cartilage and bone development. Previous work suggests that...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556258
更新日期：1991-11-01 00:00:00
abstract：:The aim of this study was to evaluate bone resorption (BR) in rats by two methods: chronic 3H-tetracycline labeling (3HTC) and pyridinium crosslink excretion (PYDX), and compare the sensitivity of these markers in two age groups. Female Sprague-Dawley rats at 12-29 weeks of age ("mature", n = 12) and at 40-57 weeks of...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900631
更新日期：1999-04-01 00:00:00
abstract：:The acute-phase response (APR) is frequently observed in patients treated with intravenous (iv) zoledronate (ZOL). We investigated whether a short course of rosuvastatin (ROSU) could attenuate the ZOL--induced APR through blocking the mevalonate pathway at a proximal level. Twenty-eight osteoporotic postmenopausal wom...
journal_title：Calcified tissue international
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00223-011-9468-2
更新日期：2011-05-01 00:00:00
abstract：:This manuscript discusses mineral and bone disorders of chronic kidney disease (MBD-CKD) in pediatric patients with special emphasis on the underlying pathophysiology, the causes and clinical profile of growth retardation, the alterations in the growth plate, the strategies to optimize growth and the medical recommend...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-020-00787-z
更新日期：2021-01-16 00:00:00
abstract：:We studied the most complete skeletons found in an excavation from the 14th and 15th century in central Stockholm. One hundred eighty-seven were from men and 156 from women: 241 individuals were estimated to be between 20 and 39 and 102 between 40 and 59 years old at death. We examined the bones radiographically and b...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00301601
更新日期：1995-05-01 00:00:00
abstract：:The Sarcopenia and Quality of Life questionnaire (SarQol®) is a self-administered multidimensional sarcopenia-specific tool designed for community-dwelling subjects aged 65 years and older. The purpose of the present study was to evaluate the psychometric properties of the Spanish version of the SarQoL®. A total of 25...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00635-9
更新日期：2020-03-01 00:00:00
abstract：:In humans, gastric surgery results in in osteopenia via mechanisms that are insufficiently understood; surgery-induced changes in the hormonal axes involving the stomach, thyroid, and the parathyroids may play a role. To study this in more detail, we evaluated calcium (Ca), magnesium (Mg), and phosphorus (P) metabolis...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900553
更新日期：1998-11-01 00:00:00
abstract：:Serum immunoreactive osteocalcin (irOC) consists of two fractions that differ from each other by their affinity for hydroxyapatite. The high and low affinity fractions are referred to as irOCbound and irOCfree, respectively. To evaluate whether these fractions are determinants for different characteristics of bone or ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900543
更新日期：1998-11-01 00:00:00
abstract：:This article reviews the effects of restrictive eating disorders on bone health. The relationship between eating disorders and amenorrhea is discussed in detail. The pathologic impact of malnutrition on bone is explored by examining the results of studies using various available imaging techniques. The multiple hormon...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-016-0164-0
更新日期：2017-05-01 00:00:00
