Abstract:
:The small proteoglycan decorin had been localized previously at the d-band in the gap zone of collagen fibrils in nonmineralizing tissues. In bone matrix this zone is proteoglycan free and is at least in some species the place where mineralization along collagen fibrils starts. To study the metabolism of the small proteoglycans decorin and biglycan under mineralizing conditions, osteoblasts from human nasal bone were cultured for several weeks in the presence or absence of β-glycerophosphate and ascorbate. An immediate consequence of the treatment was a reduced expression of decorin, as judged by immune precipitation, whereas the biosynthesis of biglycan was not affected. Pulse-chase experiments were performed with osteoblasts embedded in floating type I collagen gels. In the presence of β-glycerophosphate and ascorbate, a more rapid turnover of both proteoglycans was noted; the one of biglycan reached statistical significance. Indirect evidence for an enhanced rate of proteoglycan endocytosis was obtained. This effect was not seen in cultured skin fibroblasts. Thus, osteoblasts respond rapidly to mineralizing conditions with alterations of small proteoglycan biosynthesis and turnover.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Blumberg P,Brenner R,Budny S,Kresse Hdoi
10.1007/s002239900279subject
Has Abstractpub_date
1997-06-01 00:00:00pages
554-560issue
6eissn
0171-967Xissn
1432-0827pii
10.1007/s002239900279journal_volume
60pub_type
杂志文章abstract::Icariin, the main constituent of Herba Epimedii, appears to be a promising alternative to classic drugs used to treat osteoporosis. However, the detailed molecular mechanisms of its action and the role of icariin in the cross-talk between osteoblasts and adipocytes remain unclear. The present study was designed to inv...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-016-0138-2
更新日期:2016-08-01 00:00:00
abstract::The presence of lipids in urinary stones was determined by histochemical and biochemical methods. When crystals of calcium oxalate, made by mixing calcium chloride and potassium oxalate solutions and sections of human calcium oxalate urinary stones, were exposed to osmium vapors, there was no staining of the pure crys...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556340
更新日期:1988-02-01 00:00:00
abstract::The aim of this study was to evaluate the association among phalangeal morphometric parameters, amplitude-dependent speed of sound (AD-SOS), ultrasound bone profile index (UBPI), and spinal bone mineral density (BMD) and fracture status. One hundred women (controls, mean age 53 +/- 12 years) and 40 osteoporotic women ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-1092-0
更新日期:2003-04-01 00:00:00
abstract::In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-011-9467-3
更新日期:2011-05-01 00:00:00
abstract::N-acetyl-beta-D-glucosaminidase (NAG) is a lysosomal enzyme predominantly located in renal proximal tubules. In idiopathic hypoparathyroidism (IHP), 100 Units of human PTH (1-34) increased urinary excretion of NAG from 0.029 +/- 0.027 to 0.173 +/- 0.035 U/lGF (p less than 0.05) in two patients before treatment and fro...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556010
更新日期:1989-12-01 00:00:00
abstract::Cartilage oligomeric matrix protein (COMP) is a macromolecule of yet unknown function with restricted distribution among tissues. In the present study, the ultrastructural localization of COMP in porcine immature joint cartilage and growth plate cartilage was semiquantitatively delineated. Tissues were fixed in a mixt...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900278
更新日期:1997-06-01 00:00:00
abstract::Each of the presently available noninvasive methods used to determine the nature and degree of changes in skeletal mass has significant advantages and limitations. Dual photon absorptiometric techniques generally have a very low radiation dose. They are also less expensive to construct than other systems. However, the...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02411281
更新日期:1982-09-01 00:00:00
abstract::Patients with systemic lupus erythematosus (SLE), a chronic inflammatory disease characterized by loss of T- and B-cell tolerance to autoantigens, are at increased risk for osteoporosis and fractures. Mice deficient in Fc gamma receptor IIb (FcγRIIB) exhibit spontaneous SLE and its restoration rescues the disease. To ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-018-0464-7
更新日期:2018-12-01 00:00:00
abstract::The aim of this study was to investigate bone mineral density (BMD) and bone turnover in patients with primary knee osteoarthritis (KOA) and to compare them with generalized OA (GOA) and nonGOA patients. A total of 88 postmenopausal primary KOA patients were studied. OA was graded by using knee radiographs. BMD of the...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010068
更新日期:2000-05-01 00:00:00
abstract::Mononuclear phagocytes have been implicated as important cellular elements in the process of bone resorption. We have postulated that the recruitment and migration of mononuclear phagocytes to bone occurs via a mechanism(s) in which bone-derived chemotactic factors (BDCF) are released from foci undergoing resorption. ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02557681
更新日期:1985-01-01 00:00:00
abstract::MicroRNAs (miRNAs/miRs) are important biomarkers for the progression of intervertebral disc degeneration (IDD). We investigated the role of miR-30d in IDD progression through its interactions with forkhead box O3 (FOXO3) and C-X-C motif ligand 10 (CXCL10). We first measured the expression of miR-30d, FOXO3, and CXCL10...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00760-w
更新日期:2021-02-01 00:00:00
abstract::To determine the relationships among nutrients intake, bone mass, and bone turnover in women we have investigated these issues in a population-based, cross-sectional, observational study in one county in central Sweden. A total of 175 women aged 28-74 at entry to the study were included. Dietary assessment was made by...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298425
更新日期:1995-08-01 00:00:00
abstract::Transcriptional regulation of the postnatal skeleton is incompletely understood. Here, we determined the consequence of loss of early growth response gene 1 (EGR-1) on bone properties. Analyses were performed on both the microscopic and molecular levels utilizing micro-computed tomography (micro-CT) and Fourier transf...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-011-9486-0
更新日期:2011-07-01 00:00:00
abstract::Although hypogonadism is a risk factor for bone loss and fractures, the different etiopathophysiology and hormonal profile of classical and obesity-induced hypogonadism may lead to differences in musculoskeletal profile. This is a cross-sectional study of hypogonadal men between 40 and 74 years old. Our outcomes inclu...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-017-0316-x
更新日期:2017-12-01 00:00:00
abstract::It has recently been demonstrated that calcium ionophore A23187 mimics certain of the effects of parathyroid hormone (PTH) on bone in vitro, including stimulation of 45Ca release and cAMP formation. To further examine the relative effects of these two agents on bone cell metabolism, we compared the effects of syntheti...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02411291
更新日期:1982-09-01 00:00:00
abstract::In the past decade there have been numerous publications reporting a significant and direct relationship between handgrip strength and bone mineral density (BMD) of the proximal femur in older adults. The present report challenges the appropriateness of the methods, and thus the conclusions used in these studies. Spec...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900621
更新日期:1999-04-01 00:00:00
abstract::Until 2006 the only mutations known to cause osteogenesis imperfecta (OI) were in the two genes coding for type I collagen chains. These dominant mutations affecting the expression or primary sequence of collagen α1(I) and α2(I) chains account for over 90% of OI cases. Since then a growing list of mutant genes causing...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-013-9723-9
更新日期:2013-10-01 00:00:00
abstract::The present study was designed to characterize dentin formed in transplanted rat molars by investigating calcium (Ca), phosphorus (P), and magnesium (Mg) concentrations using electron probe microanalysis (EPMA) as well as examining the rate of dentin matrix formation by vital staining. The unerupted immature lower rig...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0134-4
更新日期:2006-03-01 00:00:00
abstract::The pharmacokinetics and bioavailability of pamidronate were assessed in patients with breast cancer, 6 subjects received the drug intravenously and 7 orally. The initial plasma half-life of pamidronate was short (42 +/- 27 min) and the apparent total plasma clearance was high (471 +/- 298 ml/min). The renal clearance...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555856
更新日期:1991-12-01 00:00:00
abstract::Hypophosphatemic rickets (HR) is a group of rare disorders caused by excessive renal phosphate wasting. The purpose of this cross-sectional study of 38 HR patients was to characterize the phenotype of adult HR patients. Moreover, skeletal and endodontic severity scores were defined to assess possible gender difference...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-010-9373-0
更新日期:2010-08-01 00:00:00
abstract::The P2X7 nucleotide receptor (P2X7R) is an ATP-gated ion channel expressed in bone cells. Homozygous null P2X7R (P2X7R(-/-)) mice have reduced bone formation, so we hypothesized that P2X7R(-/-) mice have impaired fracture healing compared to P2X7R(+/+) control mice. To test the hypothesis, adult P2X7R(-/-) mice and P2...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-009-9237-7
更新日期:2009-05-01 00:00:00
abstract::Test implant plates surgically retrieved from distal femurs of dogs were studied by Raman spectroscopy in order to characterize the bone-implant interface. The implant surface consisted of phosphate mineral, plasma sprayed on a titanium substrate. On the basis of its spectroscopic signature, the phosphate mineral of b...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556157
更新日期:1991-05-01 00:00:00
abstract::The purpose of this study is to estimate the efficacy of eldecalcitol (1α, 25-Dihydroxy-2β- (3-hydroxypropyloxy) vitamin D3; ELD) on bone metabolism after long-term administration. Six-month-old Wistar-Imamichi rats were ovariectomized (OVX) and administered ELD orally at doses of 7.5, 15, or 30 ng/kg daily. Bone mine...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-014-9937-5
更新日期:2015-01-01 00:00:00
abstract::Effects of a single dose of 200 IU of nasal salmon calcitonin (SCT) on calcium metabolism and biochemical markers of bone turnover were investigated in 12 healthy male volunteers in a randomized, placebo-controlled, cross-over design. The nasal spray was given in the morning, and subsequently blood and urine samples w...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF01320907
更新日期:1993-10-01 00:00:00
abstract::To test the hypothesis that the relationship between fat mass (FM) and bone mineral density (BMD) is mediated by leptin. The study involved 611 individuals aged 20-89 years who were randomly sampled from Ho Chi Minh City (Vietnam). BMD at the femoral neck (FN), lumbar spine (LS), and whole body (WB) was measured by DX...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-016-0196-5
更新日期:2017-01-01 00:00:00
abstract::This randomized, double-blind study assessed the antifracture efficacy and safety of intermittent intravenous (IV) ibandronate versus oral daily risedronate in Japanese patients with primary osteoporosis. Ambulatory patients aged ≥60 years were randomized to receive 0.5 or 1 mg/month IV ibandronate plus oral daily pla...
journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00223-013-9734-6
更新日期:2013-08-01 00:00:00
abstract::Osteonectin, gamma-carboxyglutamic acid-containing (Gla) protein, and dentin phosphoprotein were covalently attached to sepharose beads and inoculated in solutions at two different degrees of supersaturation with respect to both octacalcium phosphate (OCP) and hydroxyapatite. In both solutions, the inhibitory activity...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00308323
更新日期:1993-02-01 00:00:00
abstract::The goals of internal fixation are an accurate reduction and stable fixation in the presence of adequate bony vascularity. This can be achieved by a variety of means including plate fixation. A certain amount of periosteal stripping is necessary for proper open reduction of a fracture and for proper plate application....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900080
更新日期:1996-07-01 00:00:00
abstract::Changes in the acidity of osteoclasts were evaluated by direct measurement of the fluorescent intensity of osteoclasts exposed to acridine orange, a fluorescent weak base which becomes concentrated in acid-containing subcellular compartments. Parathyroid hormone (PTH) produced dose-dependent increases in fluorescent i...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554924
更新日期:1985-12-01 00:00:00
abstract::We describe here the activity of a novel selective estrogen receptor modulator, SP500263. When given to adult ovariectomized (OVX) rats for 28 days at doses of 0.3, 1, or 3 mg/kg/day, we found that SP500263 partially protected against OVX-induced loss of bone mineral content in the distal ends of femurs and in the who...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-002-1029-2
更新日期:2003-06-01 00:00:00