Abstract：
:In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of osteogenesis include two-dimensional (2D) single type cell monolayers and 3D cultures. However, an in vitro scaffold-free multicellular 3D model of osteogenesis is missing. We hypothesized that the self-inductive ossification capacity of bone marrow tissue can be harnessed in vitro and employed as a scaffold-free multicellular 3D model of osteogenesis. Therefore, rat bone marrow tissue was cultured for 28 days in three settings: 2D monolayer, 3D homogenized pellet, and 3D organotypic explant. The ossification potential of marrow in each condition was quantified by micro-computed tomography. The 3D organotypic marrow explant culture resulted in the greatest level of ossification with plate-like bone formations (up to 5 mm in diameter and 0.24 mm in thickness). To evaluate the mimicry of the organotypic marrow explants to newly forming native bone tissue, detailed compositional and morphological analyses were performed, including characterization of the ossified matrix by histochemistry, immunohistochemistry, Raman microspectroscopy, energy dispersive X-ray spectroscopy, backscattered electron microscopy, and micromechanical tests. The results indicated that the 3D organotypic marrow explant culture model mimics newly forming native bone tissue in terms of the characteristics studied. Therefore, this platform holds significant potential to be used as a model of osteogenesis, offering an alternative to in vitro monolayer cultures and in vivo animal models.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Gurkan UA,Kishore V,Condon KW,Bellido TM,Akkus Odoi
10.1007/s00223-011-9467-3subject
Has Abstractpub_date
2011-05-01 00:00:00pages
388-401issue
5eissn
0171-967Xissn
1432-0827journal_volume
88pub_type
杂志文章abstract：:The mineral in bone is located primarily within the collagen fibril, and during mineralization the fibril is formed first and then water within the fibril is replaced with mineral. Our goal is to understand the mechanism of fibril mineralization, and as a first step we recently determined the size exclusion characteri...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9085-2
更新日期：2008-02-01 00:00:00
abstract：:Yerba mate (Ilex paraguariensis) is a xanthine-containing beverage, which is also rich in caffeine. Because caffeine has a negative impact on bone mineral density (BMD) mainly associated with low calcium (Ca) diets, there would be expected a negative effect of yerba mate on bone. In this study, Sprague-Dawley rats wer...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-015-0043-0
更新日期：2015-11-01 00:00:00
abstract：:Orthopaedic wear debris induces release of bone-resorbing factors from macrophages and fibroblasts. However, the extent to which elemental metallic particles induce bone cells to express factors contributing to implant loosening remains unclear. This study showed that exposure of MG-63 osteoblast-like cells to titaniu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223001125
更新日期：2000-08-01 00:00:00
abstract：:The purpose of this study was to determine whether human parathyroid hormone (h-PTH) enhances trabecular bone mass and connectivities that were reduced by streptozotocin (STZ)-induced diabetes in rats. Seven-month-old female Wistar rats were injected with STZ or its vehicle intraperitoneally. All vehicle-injected norm...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005838
更新日期：2000-03-01 00:00:00
abstract：:Enhanced osteoclast formation and function is a fundamental cause of alterations to bone structure and plays an important role in several diseases impairing bone quality. Recent work revealed that TRP calcium channels 3 and 6 might play a special role in this context. By analyzing the bone phenotype of TRPC6-deficient...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00673-8
更新日期：2020-06-01 00:00:00
abstract：:Tumor tissue from a patient with squamous cell carcinoma of the lung and hypercalcemia has been serially implanted into athymic mice. Tumor-bearing mice develop cachexia, hypercalcemia without bone metastases, hypophosphatemia, increased urinary cyclic adenosine monophosphate (cAMP) to creatinine ratio, and undetectab...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405367
更新日期：1984-09-01 00:00:00
abstract：:Forty 5-week-old rats were randomly divided into 1 control group given a normal diet and water low in fluoride (0.026 mM/1) and experimental group receiving the same basic diet and a fluoride supplement of 2.6 mMol/l in the drinking water. After 30 weeks, half of the fluoride-supplemented and half of the nonsupplement...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405089
更新日期：1983-07-01 00:00:00
abstract：:Measurement of areal bone mineral density (aBMD) in intravertebral subregions may increase the diagnostic sensitivity of dual-energy X-ray absorptiometry (DXA)-derived parameters for vertebral fragility. This study investigated whether DXA-derived bone parameters in vertebral subregions were better predictors of verte...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-014-9866-3
更新日期：2014-08-01 00:00:00
abstract：:This article reviews the effects of restrictive eating disorders on bone health. The relationship between eating disorders and amenorrhea is discussed in detail. The pathologic impact of malnutrition on bone is explored by examining the results of studies using various available imaging techniques. The multiple hormon...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-016-0164-0
更新日期：2017-05-01 00:00:00
abstract：:Mandibular first molars from 17-day-old mouse embryos were cultivated in vitro for 10 days using a new organ culture method. This method consisted of using a small glass dish and an agar chamber to slowly float the molars to the gas-medium interface where they were maintained by the surface tension of the medium. The ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555218
更新日期：1986-10-01 00:00:00
abstract：:There are few longitudinal data on change in bone structure and muscle mass, strength and function in later life. We report these, and consider bone-muscle interrelationships in older men and women. We studied 188 men and 166 women from the Hertfordshire Cohort Study, who underwent peripheral quantitative computed tom...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0442-0
更新日期：2018-11-01 00:00:00
abstract：:Each of the presently available noninvasive methods used to determine the nature and degree of changes in skeletal mass has significant advantages and limitations. Dual photon absorptiometric techniques generally have a very low radiation dose. They are also less expensive to construct than other systems. However, the...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411281
更新日期：1982-09-01 00:00:00
abstract：:As the correlation of bone mass from childhood to adulthood is unclear, we conducted a long-term prospective observational study to determine if a pediatric bone mass scan could predict adult bone mass. We measured cortical bone mineral content (BMC [g]), bone mineral density (BMD [g/cm(2)]), and bone width (cm) in th...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-013-9802-y
更新日期：2014-02-01 00:00:00
abstract：:Glycogen storage disease type 1a (Von Gierke's disease) is one of the more common glycogen storage diseases (GSD). GSD 1a patients can have severe idiopathic osteopenia, often beginning at a young age. Since calcium tracer studies offer a sensitive probe of the bone microenvironment and of calcium deposition, kinetics...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00369209
更新日期：1996-12-01 00:00:00
abstract：:Newly developed fuzzy logic-derived structural parameters were used to characterize trabecular bone architecture in high-resolution magnetic resonance imaging (HR-MRI) of human cadaver calcaneus specimens. These parameters were compared to standard histomorphological structural measures and analyzed concerning perform...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9058-5
更新日期：2007-10-01 00:00:00
abstract：:In this cross-sectional study, 91 healthy premenopausal women aged 20-39 years were investigated to determine the effect of physical activities during their teenage years on their current bone mineral densities (BMD). We measured whole-body BMD (WBMD), lumbar BMD (LBMD), and radial BMD (RBMD) with dual energy X-ray ab...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02684999
更新日期：2001-01-01 00:00:00
abstract：:We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Gr...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-003-0110-9
更新日期：2004-03-01 00:00:00
abstract：:Osteoarthritis (OA), the most prevalent chronic joint disease, increases in prevalence with age, and affects majority of individuals over the age of 65 and is a leading musculoskeletal cause of impaired mobility in the elderly. Because the precise molecular mechanisms which are involved in the degradation of cartilage...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-014-9917-9
更新日期：2014-12-01 00:00:00
abstract：:Transcriptional regulation of the postnatal skeleton is incompletely understood. Here, we determined the consequence of loss of early growth response gene 1 (EGR-1) on bone properties. Analyses were performed on both the microscopic and molecular levels utilizing micro-computed tomography (micro-CT) and Fourier transf...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9486-0
更新日期：2011-07-01 00:00:00
abstract：:Binding sites for wheat germ agglutinin (WGA)-lectin have been shown to become revealed in the demineralized resorption lacunae that osteoclasts excavate on bone substrate. Peroxidase-conjugated WGA-lectin, which binds to bone matrix glycoconjugates and proteoglycans, was used in pit formation assays to assess the act...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00299317
更新日期：1994-11-01 00:00:00
abstract：:Proton pump inhibitors are taken by millions of patients for prevention and treatment of gastroesophageal diseases. Case-control studies have suggested that use of omeprazole is associated with an increased risk of hip fractures. The aim of this prospective study was to assess the risk of vertebral fractures in postme...
journal_title：Calcified tissue international
pub_type： 杂志文章,多中心研究
doi：10.1007/s00223-008-9188-4
更新日期：2009-01-01 00:00:00
abstract：:Diets with a high saturated fat content can produce deleterious effects on the absorption of dietary calcium and consequently an adverse effect on bone mineralization in growing animals. Hence dietary fat may have long-term consequences for skeletal health and skeletal pathologies such as osteoporosis. Whether a diet ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900492
更新日期：1998-07-01 00:00:00
abstract：:Ipriflavone (i.p.) positively affects bone density in postmenopausal osteoporosis, primarily by inhibiting bone resorption. Using in vitro models of human osteoblast differentiation, we have observed that i.p. and some of its metabolites stimulate the expression of bone sialoprotein, decorin, and type I collagen, and ...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s002239900378
更新日期：1997-01-01 00:00:00
abstract：:The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. The most abundant of these was purified to homoge...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00298593
更新日期：1995-06-01 00:00:00
abstract：:Sarcopenia and osteoporosis are two sides of the same coin. They represent different aspects of the same age-related process of musculoskeletal atrophy and together culminate in falls, fractures, deconditioning, and increased mortality in older individuals. However, the current therapeutic approach to the prevention o...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-015-9956-x
更新日期：2015-03-01 00:00:00
abstract：:SUMMARY. The high variation often observed in the ex vivo fibroblastic-colony forming unit (CFU-f) assay is likely to be due to both biological and experimental variation. To determine whether we could improve experimental methods we developed an alternative method of bone marrow cell (BMC) isolation employing a centr...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900723
更新日期：1999-11-01 00:00:00
abstract：:The effect of ipriflavone (IP), a synthetic isoflavonoid derivative, on in vivo bone formation was studied in rat perialveolar bone by surgically producing a hole in the mandibular bone. The holes were filled either with powdered IP or with compounds containing no osteoinductive properties such as biostite and Htr (ha...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900533
更新日期：1998-10-01 00:00:00
abstract：:Low-grade inflammation negatively affects bone. Resveratrol is a natural compound proven to possess both anti-inflammatory and bone protective properties. However, it is uncertain if the bone effects are mediated though anti-inflammatory effects. Firstly, we investigated if resveratrol affects proliferation and differ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-016-0130-x
更新日期：2016-08-01 00:00:00
abstract：:Insulin is a potent stimulator of collagen synthesis and other osteoblastic cell functions. In various insulin-sensitive tissues, stimulation of glucose transport and glycolytic metabolism are hallmarks of insulin action and may play a role in insulin regulation of cellular function. However, the effects of insulin on...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556657
更新日期：1989-07-01 00:00:00
abstract：:The aim of this study was to analyze the efficacy of using rhBMP-2 (recombinant human morphogenetic protein-2) in the treatment of patients with cleft lip and palate defects (CLPD). Seven databases were screened: PubMed (Medline), Lilacs, Ibecs, Web of Science, BBO, Scopus, and The Cochrane Library. Clinical trials th...
journal_title：Calcified tissue international
pub_type： 杂志文章,meta分析
doi：10.1007/s00223-018-0486-1
更新日期：2019-02-01 00:00:00
