Abstract:
:In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of osteogenesis include two-dimensional (2D) single type cell monolayers and 3D cultures. However, an in vitro scaffold-free multicellular 3D model of osteogenesis is missing. We hypothesized that the self-inductive ossification capacity of bone marrow tissue can be harnessed in vitro and employed as a scaffold-free multicellular 3D model of osteogenesis. Therefore, rat bone marrow tissue was cultured for 28 days in three settings: 2D monolayer, 3D homogenized pellet, and 3D organotypic explant. The ossification potential of marrow in each condition was quantified by micro-computed tomography. The 3D organotypic marrow explant culture resulted in the greatest level of ossification with plate-like bone formations (up to 5 mm in diameter and 0.24 mm in thickness). To evaluate the mimicry of the organotypic marrow explants to newly forming native bone tissue, detailed compositional and morphological analyses were performed, including characterization of the ossified matrix by histochemistry, immunohistochemistry, Raman microspectroscopy, energy dispersive X-ray spectroscopy, backscattered electron microscopy, and micromechanical tests. The results indicated that the 3D organotypic marrow explant culture model mimics newly forming native bone tissue in terms of the characteristics studied. Therefore, this platform holds significant potential to be used as a model of osteogenesis, offering an alternative to in vitro monolayer cultures and in vivo animal models.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Gurkan UA,Kishore V,Condon KW,Bellido TM,Akkus Odoi
10.1007/s00223-011-9467-3subject
Has Abstractpub_date
2011-05-01 00:00:00pages
388-401issue
5eissn
0171-967Xissn
1432-0827journal_volume
88pub_type
杂志文章abstract::A case-control study was undertaken to understand the etiopathology of the bone deformities among young children in a fluoride-affected village of the Bihar State. Two villages were selected: one village with high fluoride in drinking water (7.9 +/- 4.15 ppm), and the other village with normal levels of fluoride (0.6 ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0233-2
更新日期:2005-06-01 00:00:00
abstract::To characterize osteopenic changes in ovariectomized (OVX) rats as a function of time, female Sprague Dawley rats (240 g body weight, 90 days old) were subjected to bilateral ovariectomy or sham surgery and killed at various times from 14-180 days postovariectomy. The proximal tibial metaphysis was processed undecalci...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02571317
更新日期:1988-09-01 00:00:00
abstract::Previous studies have indicated that children with inflammatory bowel disease (IBD) may not achieve optimal bone mass. We evaluated the skeletal characteristics in children and adolescents with IBD. This cross-sectional cohort study comprised 80 IBD patients (median age 14.9 years, range 5-20) with a median disease du...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-012-9617-2
更新日期:2012-08-01 00:00:00
abstract::Mandibular first molars of 17-day-old mouse embryos were cultured in vitro to examine the histological effects of various concentrations of parathyroid hormone (PTH) on odontogenesis of the molars. PTH did not affect the cytodifferentiation of mesenchymal cells into preodontoblasts but inhibited that of preodontoblast...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555728
更新日期:1987-01-01 00:00:00
abstract::Bone involvement is one of the most disabling aspects of type I Gaucher disease and its pathophysiology is still not well understood. As an invasive procedure, bone biopsies are not appropriate in a large population study. The development of sensitive bone resorption and formation tests have allowed the authors to stu...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-2072-0
更新日期:2003-03-01 00:00:00
abstract::Aging and sex hormones related changes lead to inflammatory and oxidant conditions, which are involved in the pathogenesis of osteoporosis. Recent studies have suggested that polyphenols may exert a protective effect in such conditions. We assessed the effect of phloridzin (Phlo), a flavonoid exclusively found in appl...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0060-5
更新日期:2005-11-01 00:00:00
abstract::Studies of the effect of parathyroidectomy (PTX) on bone turnover in patients with the combination of primary hyperparathyroidism (PHPT) and Paget's disease (PD) are largely limited to case reports. The etiology of the combination is disputed. We report 30 patients and their biochemical (n = 17) and histomorphometric ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900728
更新日期:1999-12-01 00:00:00
abstract::Bone formation in a variety of contexts depends on angiogenesis; however, there are few reports of the vascular response to osteogenic skeletal loading. We used the rat forelimb compression model to characterize vascular changes after fatigue loading. The right forelimbs of 72 adult rats were loaded cyclically in vivo...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-007-9031-3
更新日期:2007-06-01 00:00:00
abstract::Osteoclastic bone resorption can be induced within the auditory bulla of the Mongolian gerbil by minute increases in air pressure. The objective of this study was to determine the rate at which osteoclasts are induced in areas of resorption and to compare the rate of osteoblast recruitment. Stainless steel tubes were ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556026
更新日期:1989-11-01 00:00:00
abstract::Tartrate resistant acid phosphatase (TRAP) has been proposed as a new biochemical marker for bone resorption. We have compared this new marker, TRAP, with the classical biochemical markers of bone remodelling, serum alkaline phosphatase (sAP), serum osteocalcin (sBGP), and with the urinary hydroxyproline/creatinine ra...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555896
更新日期:1991-07-01 00:00:00
abstract::The aim of our study was to compare the results provided by the measurement of vertebral and femoral bone mineral density (BMD) for assessing the individual risk of osteoporosis as defined by either low BMD and/or rapid bone loss. Vertebral and femoral BMD were measured twice at a mean interval of 21 months in 85 norm...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00310196
更新日期:1993-05-01 00:00:00
abstract::Biochemical markers applicable to the ovariectomized rat model can provide important tools for studying the bone remodeling process in this animal model of postmenopausal osteoporosis. We describe the development and application of two biochemical markers, a C-telopeptide (of type-I collagen) enzyme-linked immunosorbe...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010088
更新日期:2000-06-01 00:00:00
abstract::This study sought to confirm that osteoblasts of C3H/HeJ (C3H) mice, which have higher differentiation status and bone-forming ability compared to C57BL/6J (B6) osteoblasts, also have a lower apoptosis level and to test whether the higher differentiation status and bone-forming ability of C3H osteoblasts were related ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0303-5
更新日期:2006-05-01 00:00:00
abstract::This randomized, double-blind study assessed the antifracture efficacy and safety of intermittent intravenous (IV) ibandronate versus oral daily risedronate in Japanese patients with primary osteoporosis. Ambulatory patients aged ≥60 years were randomized to receive 0.5 or 1 mg/month IV ibandronate plus oral daily pla...
journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00223-013-9734-6
更新日期:2013-08-01 00:00:00
abstract::Seventy-two eugonadal women, mean age 39 +/- 4 years, with a z score for total body bone mineral content (BMC) of less than -1.5 (mean -1.80 +/- 0.12) were selected from a previous screening study of normal values of total body and regional BMC in women using dual-energy X-ray absorptiometry. The women were distribute...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
doi:10.1007/BF00334327
更新日期:1994-06-01 00:00:00
abstract::Hip fracture may cause and/or complicate institutionalization. We undertook this study to define its overall prevalence among the residents of four nursing homes in Central Italy as well as its latency and impact on mobility when it occurred within institutions. We also performed a case control analysis with the aim o...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230001212
更新日期:2001-06-01 00:00:00
abstract::Bone and mineral metabolism has been reported to affect the development of the ossification of the posterior longitudinal ligament (OPLL). The aim of this study was to compare bone mineral densities (BMD) and rate of osteoporosis between cervical OPLL and a matched control group. We also investigated the correlation o...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-012-9662-x
更新日期:2013-01-01 00:00:00
abstract::Mandibular first molars from 17-day-old mouse embryos were cultivated in vitro for 10 days using a new organ culture method. This method consisted of using a small glass dish and an agar chamber to slowly float the molars to the gas-medium interface where they were maintained by the surface tension of the medium. The ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555218
更新日期:1986-10-01 00:00:00
abstract::Previous observational studies have identified various risk factors associated with the development of osteoporosis, including sex hormone-binding globulin (SHBG). The aim of this study was to determine the potential causal effects of circulating SHBG concentrations on bone mineral density (BMD). Two-sample Mendelian ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00770-8
更新日期:2020-10-17 00:00:00
abstract::Our knowledge of the developmental changes in the concentration of serum and bone osteocalcin (OC) is limited. To investigate the interrelationship between skeletal and circulatory OC during acquisition of peak bone density in mice, we examined the temporal changes in the concentration of serum and bone OC from 3 to 1...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-1106-y
更新日期:2002-11-01 00:00:00
abstract::The goals of internal fixation are an accurate reduction and stable fixation in the presence of adequate bony vascularity. This can be achieved by a variety of means including plate fixation. A certain amount of periosteal stripping is necessary for proper open reduction of a fracture and for proper plate application....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900080
更新日期:1996-07-01 00:00:00
abstract::Humanin (HN), a mitochondrial derived peptide, plays cyto-protective role under various stress. In this study, we aimed to investigate the effects of HNGF6A, an analogue of HN, on osteoblast apoptosis and differentiation and the underlying mechanisms. Cell proliferation of murine osteoblastic cell line MC3TC-E1 was ex...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00660-z
更新日期:2020-05-01 00:00:00
abstract::The purpose of this study was to determine whether human parathyroid hormone (h-PTH) enhances trabecular bone mass and connectivities that were reduced by streptozotocin (STZ)-induced diabetes in rats. Seven-month-old female Wistar rats were injected with STZ or its vehicle intraperitoneally. All vehicle-injected norm...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/pl00005838
更新日期:2000-03-01 00:00:00
abstract::A simple method for determining histamine and polyamines in various tissues was devised. The method, however, could not be applied to calcified tissues, because the high concentration of Ca2+ in the extract interferes with the chromatographic separation of these amines. By treating the extracts from calcified tissues ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00296220
更新日期:1992-07-01 00:00:00
abstract::Gnathodiaphyseal dysplasia (GDD; OMIM#166260) is a rare skeletal disorder which is mainly characterized by cemento-osseous lesions in mandibles, bone fragility, bowing and diaphyseal sclerosis of tubular bones. GDD is caused by point mutations in Anoctamin-5 (ANO5); however, the disease mechanisms remain unclear. Here...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-019-00528-x
更新日期:2019-06-01 00:00:00
abstract::The purpose of the present study was to determine if the type of dietary fat can modify the fatty acid composition and arachidonic acid levels in the alveolar bone phospholipids. Three groups of rats were fed nutritionally adequate semipurified diets containing different lipids: 10% corn oil (control, group I, rich in...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF01351838
更新日期:1993-11-01 00:00:00
abstract::Currently used diagnostic measures for sarcopenia are based on the evaluation of appendicular skeletal muscle mass (ASMM) divided by height-squared (ASMMI). This study aimed to investigate the associations between different operational definitions of appendicular muscle mass and BMD at different skeletal sites in agin...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-014-9902-3
更新日期:2014-10-01 00:00:00
abstract::We describe a tall-statured 14-year-old boy who illustrated the full phenotypic and radiographic features of Stickler syndrome type I. A bone biopsy showed evidence of reduced bone mass and bone turnover, such as reduced BV/TV (-43%), TbTh (-29%), and OS/BS (-48%), Ob.S/BS (-27%), and Oc/BS (-47%) compared to "age-mat...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-009-9324-9
更新日期:2010-02-01 00:00:00
abstract::Results from previous quasi-static mechanical tests indicate that femurs from elderly subjects fail in vitro at forces 50% below those available in a fall from standing height. However, bone is a rate-dependent material, and it is not known whether this imbalance is present at rates of loading which occur in a fall. B...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
doi:10.1007/BF00310169
更新日期:1994-07-01 00:00:00
abstract::Although treatment with intranasal salmon calcitonin (sCT) has been shown to effectively inhibit postmenopausal bone loss, there is still controversy over both timing and the duration of its application. In an open prospective study, we therefore assessed the effect of shortterm intranasal sCT on postmenopausal bone t...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02508638
更新日期:1996-04-01 00:00:00