A scaffold-free multicellular three-dimensional in vitro model of osteogenesis.


:In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of osteogenesis include two-dimensional (2D) single type cell monolayers and 3D cultures. However, an in vitro scaffold-free multicellular 3D model of osteogenesis is missing. We hypothesized that the self-inductive ossification capacity of bone marrow tissue can be harnessed in vitro and employed as a scaffold-free multicellular 3D model of osteogenesis. Therefore, rat bone marrow tissue was cultured for 28 days in three settings: 2D monolayer, 3D homogenized pellet, and 3D organotypic explant. The ossification potential of marrow in each condition was quantified by micro-computed tomography. The 3D organotypic marrow explant culture resulted in the greatest level of ossification with plate-like bone formations (up to 5 mm in diameter and 0.24 mm in thickness). To evaluate the mimicry of the organotypic marrow explants to newly forming native bone tissue, detailed compositional and morphological analyses were performed, including characterization of the ossified matrix by histochemistry, immunohistochemistry, Raman microspectroscopy, energy dispersive X-ray spectroscopy, backscattered electron microscopy, and micromechanical tests. The results indicated that the 3D organotypic marrow explant culture model mimics newly forming native bone tissue in terms of the characteristics studied. Therefore, this platform holds significant potential to be used as a model of osteogenesis, offering an alternative to in vitro monolayer cultures and in vivo animal models.


Calcif Tissue Int


Gurkan UA,Kishore V,Condon KW,Bellido TM,Akkus O




Has Abstract


2011-05-01 00:00:00












  • Severe bone deformities in young children from vitamin D deficiency and fluorosis in Bihar-India.

    abstract::A case-control study was undertaken to understand the etiopathology of the bone deformities among young children in a fluoride-affected village of the Bihar State. Two villages were selected: one village with high fluoride in drinking water (7.9 +/- 4.15 ppm), and the other village with normal levels of fluoride (0.6 ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Khandare AL,Harikumar R,Sivakumar B

    更新日期:2005-06-01 00:00:00

  • Temporal relationship between bone loss and increased bone turnover in ovariectomized rats.

    abstract::To characterize osteopenic changes in ovariectomized (OVX) rats as a function of time, female Sprague Dawley rats (240 g body weight, 90 days old) were subjected to bilateral ovariectomy or sham surgery and killed at various times from 14-180 days postovariectomy. The proximal tibial metaphysis was processed undecalci...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Wronski TJ,Cintrón M,Dann LM

    更新日期:1988-09-01 00:00:00

  • Impaired bone health in inflammatory bowel disease: a case-control study in 80 pediatric patients.

    abstract::Previous studies have indicated that children with inflammatory bowel disease (IBD) may not achieve optimal bone mass. We evaluated the skeletal characteristics in children and adolescents with IBD. This cross-sectional cohort study comprised 80 IBD patients (median age 14.9 years, range 5-20) with a median disease du...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Laakso S,Valta H,Verkasalo M,Toiviainen-Salo S,Viljakainen H,Mäkitie O

    更新日期:2012-08-01 00:00:00

  • Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar tooth in vitro.

    abstract::Mandibular first molars of 17-day-old mouse embryos were cultured in vitro to examine the histological effects of various concentrations of parathyroid hormone (PTH) on odontogenesis of the molars. PTH did not affect the cytodifferentiation of mesenchymal cells into preodontoblasts but inhibited that of preodontoblast...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Sakakura Y

    更新日期:1987-01-01 00:00:00

  • Bone marker alterations in patients with type 1 Gaucher disease.

    abstract::Bone involvement is one of the most disabling aspects of type I Gaucher disease and its pathophysiology is still not well understood. As an invasive procedure, bone biopsies are not appropriate in a large population study. The development of sensitive bone resorption and formation tests have allowed the authors to stu...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Ciana G,Martini C,Leopaldi A,Tamaro G,Katouzian F,Ronfani L,Bembi B

    更新日期:2003-03-01 00:00:00

  • Prevention of bone loss by phloridzin, an apple polyphenol, in ovariectomized rats under inflammation conditions.

    abstract::Aging and sex hormones related changes lead to inflammatory and oxidant conditions, which are involved in the pathogenesis of osteoporosis. Recent studies have suggested that polyphenols may exert a protective effect in such conditions. We assessed the effect of phloridzin (Phlo), a flavonoid exclusively found in appl...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Puel C,Quintin A,Mathey J,Obled C,Davicco MJ,Lebecque P,Kati-Coulibaly S,Horcajada MN,Coxam V

    更新日期:2005-11-01 00:00:00

  • Thirty cases of concurrent Paget's disease and primary hyperparathyroidism: sex distribution, histomorphometry, and prediction of the skeletal response to parathyroidectomy.

    abstract::Studies of the effect of parathyroidectomy (PTX) on bone turnover in patients with the combination of primary hyperparathyroidism (PHPT) and Paget's disease (PD) are largely limited to case reports. The etiology of the combination is disputed. We report 30 patients and their biochemical (n = 17) and histomorphometric ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Gutteridge DH,Gruber HE,Kermode DG,Worth GK

    更新日期:1999-12-01 00:00:00

  • Damaging fatigue loading stimulates increases in periosteal vascularity at sites of bone formation in the rat ulna.

    abstract::Bone formation in a variety of contexts depends on angiogenesis; however, there are few reports of the vascular response to osteogenic skeletal loading. We used the rat forelimb compression model to characterize vascular changes after fatigue loading. The right forelimbs of 72 adult rats were loaded cyclically in vivo...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Matsuzaki H,Wohl GR,Novack DV,Lynch JA,Silva MJ

    更新日期:2007-06-01 00:00:00

  • Rapid induction of localized bone resorption in the auditory bulla of the Mongolian gerbil, Meriones unguiculatus, by increased air pressure.

    abstract::Osteoclastic bone resorption can be induced within the auditory bulla of the Mongolian gerbil by minute increases in air pressure. The objective of this study was to determine the rate at which osteoclasts are induced in areas of resorption and to compare the rate of osteoblast recruitment. Stainless steel tubes were ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Chole RA,Chan DE

    更新日期:1989-11-01 00:00:00

  • Clinical usefulness of serum tartrate-resistant acid phosphatase in Paget's disease of bone: correlation with other biochemical markers of bone remodelling.

    abstract::Tartrate resistant acid phosphatase (TRAP) has been proposed as a new biochemical marker for bone resorption. We have compared this new marker, TRAP, with the classical biochemical markers of bone remodelling, serum alkaline phosphatase (sAP), serum osteocalcin (sBGP), and with the urinary hydroxyproline/creatinine ra...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Torres R,de la Piedra C,Rapado A

    更新日期:1991-07-01 00:00:00

  • Spine and femur densitometry at the menopause: are both sites necessary in the assessment of the risk of osteoporosis?

    abstract::The aim of our study was to compare the results provided by the measurement of vertebral and femoral bone mineral density (BMD) for assessing the individual risk of osteoporosis as defined by either low BMD and/or rapid bone loss. Vertebral and femoral BMD were measured twice at a mean interval of 21 months in 85 norm...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Pouilles JM,Tremollieres F,Ribot C

    更新日期:1993-05-01 00:00:00

  • Development and application of a serum C-telopeptide and osteocalcin assay to measure bone turnover in an ovariectomized rat model.

    abstract::Biochemical markers applicable to the ovariectomized rat model can provide important tools for studying the bone remodeling process in this animal model of postmenopausal osteoporosis. We describe the development and application of two biochemical markers, a C-telopeptide (of type-I collagen) enzyme-linked immunosorbe...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Srivastava AK,Bhattacharyya S,Castillo G,Wergedal J,Mohan S,Baylink DJ

    更新日期:2000-06-01 00:00:00

  • High osteoblastic activity in C3H/HeJ mice compared to C57BL/6J mice is associated with low apoptosis in C3H/HeJ osteoblasts.

    abstract::This study sought to confirm that osteoblasts of C3H/HeJ (C3H) mice, which have higher differentiation status and bone-forming ability compared to C57BL/6J (B6) osteoblasts, also have a lower apoptosis level and to test whether the higher differentiation status and bone-forming ability of C3H osteoblasts were related ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Sheng MH,Lau KH,Mohan S,Baylink DJ,Wergedal JE

    更新日期:2006-05-01 00:00:00

  • Clinical efficacy on fracture risk and safety of 0.5 mg or 1 mg/month intravenous ibandronate versus 2.5 mg/day oral risedronate in patients with primary osteoporosis.

    abstract::This randomized, double-blind study assessed the antifracture efficacy and safety of intermittent intravenous (IV) ibandronate versus oral daily risedronate in Japanese patients with primary osteoporosis. Ambulatory patients aged ≥60 years were randomized to receive 0.5 or 1 mg/month IV ibandronate plus oral daily pla...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,随机对照试验


    authors: Nakamura T,Nakano T,Ito M,Hagino H,Hashimoto J,Tobinai M,Mizunuma H,MOVER Study Group.

    更新日期:2013-08-01 00:00:00

  • Longitudinal study of the effect of calcium pidolate on bone mass in eugonadal women.

    abstract::Seventy-two eugonadal women, mean age 39 +/- 4 years, with a z score for total body bone mineral content (BMC) of less than -1.5 (mean -1.80 +/- 0.12) were selected from a previous screening study of normal values of total body and regional BMC in women using dual-energy X-ray absorptiometry. The women were distribute...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章


    authors: Rico H,Revilla M,Villa LF,Alvarez de Buergo M,Arribas I

    更新日期:1994-06-01 00:00:00

  • Hip fracture in nursing homes: an Italian study on prevalence, latency, risk factors, and impact on mobility.

    abstract::Hip fracture may cause and/or complicate institutionalization. We undertook this study to define its overall prevalence among the residents of four nursing homes in Central Italy as well as its latency and impact on mobility when it occurred within institutions. We also performed a case control analysis with the aim o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Maggio D,Ubaldi E,Simonelli G,Cenci S,Pedone C,Cherubini A

    更新日期:2001-06-01 00:00:00

  • Increased bone mineral density and decreased prevalence of osteoporosis in cervical ossification of the posterior longitudinal ligament: a case-control study.

    abstract::Bone and mineral metabolism has been reported to affect the development of the ossification of the posterior longitudinal ligament (OPLL). The aim of this study was to compare bone mineral densities (BMD) and rate of osteoporosis between cervical OPLL and a matched control group. We also investigated the correlation o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Sohn S,Chung CK

    更新日期:2013-01-01 00:00:00

  • A new culture method assuring the three-dimensional development of the mouse embryonic molar tooth in vitro.

    abstract::Mandibular first molars from 17-day-old mouse embryos were cultivated in vitro for 10 days using a new organ culture method. This method consisted of using a small glass dish and an agar chamber to slowly float the molars to the gas-medium interface where they were maintained by the surface tension of the medium. The ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Sakakura Y

    更新日期:1986-10-01 00:00:00

  • Genetically Predicted Sex Hormone-Binding Globulin and Bone Mineral Density: A Mendelian Randomization Study.

    abstract::Previous observational studies have identified various risk factors associated with the development of osteoporosis, including sex hormone-binding globulin (SHBG). The aim of this study was to determine the potential causal effects of circulating SHBG concentrations on bone mineral density (BMD). Two-sample Mendelian ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Qu Z,Jiang J,Yang F,Huang J,Zhao J,Yan S

    更新日期:2020-10-17 00:00:00

  • Opposing changes in osteocalcin levels in bone vs serum during the acquisition of peak bone density in C3H/HeJ and C57BL/6J mice.

    abstract::Our knowledge of the developmental changes in the concentration of serum and bone osteocalcin (OC) is limited. To investigate the interrelationship between skeletal and circulatory OC during acquisition of peak bone density in mice, we examined the temporal changes in the concentration of serum and bone OC from 3 to 1...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Li X,Srivastava AK,Gu W,Masinde G,Mohan S,Baylink DJ

    更新日期:2002-11-01 00:00:00

  • Effect of periosteal stripping on cortical bone perfusion: a laser doppler study in sheep.

    abstract::The goals of internal fixation are an accurate reduction and stable fixation in the presence of adequate bony vascularity. This can be achieved by a variety of means including plate fixation. A certain amount of periosteal stripping is necessary for proper open reduction of a fracture and for proper plate application....

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Kowalski MJ,Schemitsch EH,Kregor PJ,Senft D,Swiontkowski MF

    更新日期:1996-07-01 00:00:00

  • HNGF6A Inhibits Oxidative Stress-Induced MC3T3-E1 Cell Apoptosis and Osteoblast Phenotype Inhibition by Targeting Circ_0001843/miR-214 Pathway.

    abstract::Humanin (HN), a mitochondrial derived peptide, plays cyto-protective role under various stress. In this study, we aimed to investigate the effects of HNGF6A, an analogue of HN, on osteoblast apoptosis and differentiation and the underlying mechanisms. Cell proliferation of murine osteoblastic cell line MC3TC-E1 was ex...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Zhu X,Zhao Z,Zeng C,Chen B,Huang H,Chen Y,Zhou Q,Yang L,Lv J,Zhang J,Pan D,Shen J,Duque G,Cai D

    更新日期:2020-05-01 00:00:00

  • Histomorphometric evaluation of the recovering effect of human parathyroid hormone (1-34) on bone structure and turnover in streptozotocin-induced diabetic rats.

    abstract::The purpose of this study was to determine whether human parathyroid hormone (h-PTH) enhances trabecular bone mass and connectivities that were reduced by streptozotocin (STZ)-induced diabetes in rats. Seven-month-old female Wistar rats were injected with STZ or its vehicle intraperitoneally. All vehicle-injected norm...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Tsuchida T,Sato K,Miyakoshi N,Abe T,Kudo T,Tamura Y,Kasukawa Y,Suzuki K

    更新日期:2000-03-01 00:00:00

  • Determination of histamine and polyamines in calcified tissues of mice: contribution of mast cells and histidine decarboxylase to the amount of histamine in the bone.

    abstract::A simple method for determining histamine and polyamines in various tissues was devised. The method, however, could not be applied to calcified tissues, because the high concentration of Ca2+ in the extract interferes with the chromatographic separation of these amines. By treating the extracts from calcified tissues ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Endo Y,Kikuchi T,Nakamura M,Shinoda H

    更新日期:1992-07-01 00:00:00

  • Genetic Disruption of Anoctamin 5 in Mice Replicates Human Gnathodiaphyseal Dysplasia (GDD).

    abstract::Gnathodiaphyseal dysplasia (GDD; OMIM#166260) is a rare skeletal disorder which is mainly characterized by cemento-osseous lesions in mandibles, bone fragility, bowing and diaphyseal sclerosis of tubular bones. GDD is caused by point mutations in Anoctamin-5 (ANO5); however, the disease mechanisms remain unclear. Here...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Wang X,Liu X,Dong R,Liang C,Reichenberger EJ,Hu Y

    更新日期:2019-06-01 00:00:00

  • Fatty acid composition and arachidonic acid concentrations in alveolar bone of rats fed diets with different lipids.

    abstract::The purpose of the present study was to determine if the type of dietary fat can modify the fatty acid composition and arachidonic acid levels in the alveolar bone phospholipids. Three groups of rats were fed nutritionally adequate semipurified diets containing different lipids: 10% corn oil (control, group I, rich in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Alam SQ,Kokkinos PP,Alam BS

    更新日期:1993-11-01 00:00:00

  • Gender-specific associations of appendicular muscle mass with BMD in elderly Italian subjects.

    abstract::Currently used diagnostic measures for sarcopenia are based on the evaluation of appendicular skeletal muscle mass (ASMM) divided by height-squared (ASMMI). This study aimed to investigate the associations between different operational definitions of appendicular muscle mass and BMD at different skeletal sites in agin...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Gonnelli S,Caffarelli C,Cappelli S,Rossi S,Giordano N,Nuti R

    更新日期:2014-10-01 00:00:00

  • Evidence of reduced bone turnover and disturbed mineralization process in a boy with Stickler syndrome.

    abstract::We describe a tall-statured 14-year-old boy who illustrated the full phenotypic and radiographic features of Stickler syndrome type I. A bone biopsy showed evidence of reduced bone mass and bone turnover, such as reduced BV/TV (-43%), TbTh (-29%), and OS/BS (-48%), Ob.S/BS (-27%), and Oc/BS (-47%) compared to "age-mat...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Al Kaissi A,Roschger P,Nawrot-Wawrzyniak K,Krebs A,Grill F,Klaushofer K

    更新日期:2010-02-01 00:00:00

  • Effects of loading rate on strength of the proximal femur.

    abstract::Results from previous quasi-static mechanical tests indicate that femurs from elderly subjects fail in vitro at forces 50% below those available in a fall from standing height. However, bone is a rate-dependent material, and it is not known whether this imbalance is present at rates of loading which occur in a fall. B...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章


    authors: Courtney AC,Wachtel EF,Myers ER,Hayes WC

    更新日期:1994-07-01 00:00:00

  • The effect of intranasal salmon calcitonin on postmenopausal bone turnover as assessed by biochemical markers: evidence of maximal effect after 8 weeks of continuous treatment.

    abstract::Although treatment with intranasal salmon calcitonin (sCT) has been shown to effectively inhibit postmenopausal bone loss, there is still controversy over both timing and the duration of its application. In an open prospective study, we therefore assessed the effect of shortterm intranasal sCT on postmenopausal bone t...

    journal_title:Calcified tissue international

    pub_type: 杂志文章


    authors: Kraenzlin ME,Seibel MJ,Trechsel U,Boerlin V,Azria M,Kraenzlin CA,Haas HG

    更新日期:1996-04-01 00:00:00