Abstract:
:It has recently been demonstrated that calcium ionophore A23187 mimics certain of the effects of parathyroid hormone (PTH) on bone in vitro, including stimulation of 45Ca release and cAMP formation. To further examine the relative effects of these two agents on bone cell metabolism, we compared the effects of synthetic PTH 1-34 (50 ng/ml) and calcium ionophore A23187 (0.5 micrograms/ml) on 45Ca release, DNA concentration, and nucleic acid synthesis in fetal rat forelimb rudiments cultured for periods up to 120 h. Both agents stimulated 45Ca release; however, the effects of PTH were apparent after a shorter period of exposure. Bone DNA concentration (expressed as microgram DNA/mg bone) was not affected by PTH but was significantly increased relative to control values by exposure to A23187 for 8-120 h of incubation. PTH increased the incorporation of 3H-thymidine into DNA at 30 and 48 h, and increased the incorporation of 14C-uridine into RNA at 48 h, time points which corresponded to a period of accelerated PTH stimulation of 45Ca release. In contrast, 3H-thymidine and 14C-uridine incorporation were both uniformly suppressed by A23187 at all time points examined. Thus the increased DNA concentration observed in A23187-treated rudiments appeared to be the result of a decreased rate of bone maturation and mineralization. The markedly different patterns of nucleic acid synthesis in response to PTH and A23187 suggest that these agents differ significantly in their mechanisms of action on bone cell metabolism.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
DeBartolo TF,Pegg LE,Shasserre C,Hahn TJdoi
10.1007/BF02411291subject
Has Abstractpub_date
1982-09-01 00:00:00pages
495-500issue
5eissn
0171-967Xissn
1432-0827journal_volume
34pub_type
杂志文章abstract::The aim of this study was to follow the changes in bone mineral density (BMD) and biochemical markers of bone turnover in 10 children (7.5-17.5 years of age) with severe juvenile idiopathic arthritis (JIA), during a 3-year therapy with salmon calcitonin (100 IU/day 2 months on and 2 off for a year and 200 IU/day for 2...
journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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doi:10.1007/BF02557834
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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pub_type: 杂志文章,随机对照试验
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更新日期:2013-08-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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更新日期:1990-11-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555819
更新日期:1990-01-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-010-9393-9
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02553763
更新日期:1989-04-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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更新日期:1999-01-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00334485
更新日期:1992-10-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900148
更新日期:1996-11-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
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更新日期:2007-05-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2001-10-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-002-1051-4
更新日期:2003-09-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-002-2098-y
更新日期:2003-10-01 00:00:00