Abstract:
:Cartilage oligomeric matrix protein (COMP) is a macromolecule of yet unknown function with restricted distribution among tissues. In the present study, the ultrastructural localization of COMP in porcine immature joint cartilage and growth plate cartilage was semiquantitatively delineated. Tissues were fixed in a mixture of low concentration glutar- and paraformaldehyde, embedded at low temperature, and subjected to immunocytochemistry using polyclonal antibodies raised against bovine COMP. Protein A-coated colloidal gold was used for detection. The most intense immunolabeling for COMP was noted in the proliferative zones of the growth cartilages. Here the concentration of immunomarker was higher in the territorial compartment than in the pericellular and interterritorial areas. A low concentration of COMP was observed in the resting and hypertrophic zones. The immunolabeling for COMP did not differ between the three matrix compartments of these zones. Supported by previous data obtained by in situ hybridization, the concentration of immunolabeling in the proliferative zone indicates a high rate of COMP synthesis in proliferative chondrocytes. Hence, COMP may be considered as a marker for normal differentiation into proliferative chondrocytes.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Ekman S,Reinholt FP,Hultenby K,Heinegârd Ddoi
10.1007/s002239900278subject
Has Abstractpub_date
1997-06-01 00:00:00pages
547-53issue
6eissn
0171-967Xissn
1432-0827journal_volume
60pub_type
杂志文章abstract::Impact activity is an important contributor to bone accrual. Children engaging in such activity have been shown to have greater bone mineral density (BMD) than their peers. This cross-sectional study was designed to quantify the association between BMD and impact activity in pre-pubescent girls, specifically examining...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-1131-x
更新日期:2003-01-01 00:00:00
abstract::Psoriatic arthritis (PsA) is an inflammatory rheumatic disorder that occurs in patients with psoriasis and predominantly affects musculoskeletal structures, skin, and nails. The etiology of PsA is not well understood but evidence supports an interplay of genetic, immunologic, and environmental factors which promote pa...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-017-0380-2
更新日期:2018-05-01 00:00:00
abstract::The purpose of this study is to estimate the efficacy of eldecalcitol (1α, 25-Dihydroxy-2β- (3-hydroxypropyloxy) vitamin D3; ELD) on bone metabolism after long-term administration. Six-month-old Wistar-Imamichi rats were ovariectomized (OVX) and administered ELD orally at doses of 7.5, 15, or 30 ng/kg daily. Bone mine...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-014-9937-5
更新日期:2015-01-01 00:00:00
abstract::Osteonectin, gamma-carboxyglutamic acid-containing (Gla) protein, and dentin phosphoprotein were covalently attached to sepharose beads and inoculated in solutions at two different degrees of supersaturation with respect to both octacalcium phosphate (OCP) and hydroxyapatite. In both solutions, the inhibitory activity...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00308323
更新日期:1993-02-01 00:00:00
abstract::Pregnancy and lactation-associated osteoporosis (PAO) is a rare condition with little known pathophysiology. Most cases are diagnosed in the third trimester of pregnancy or in the first weeks postpartum, particularly in first pregnancies. Vertebral fractures are most commonly observed and characterised by prolonged se...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-015-0028-z
更新日期:2015-10-01 00:00:00
abstract::Genome-wide association studies (GWAS) have demonstrated that genetic variation at the MADS box transcription enhancer factor 2, polypeptide C (MEF2C) locus is robustly associated with bone mineral density, primarily at the femoral neck. MEF2C is a transcription factor known to operate via the Wnt signaling pathway. O...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-013-9824-5
更新日期:2014-04-01 00:00:00
abstract::Mature dental enamel is the most mineralized of all mammalian tissues and considered to be free of collagen. Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are two nonreducible cross-links of mature collagen. Hydroxyproline (Hyp) is an amino acid that is believed to be indicative of the presence of collagen....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-004-0122-0
更新日期:2005-02-01 00:00:00
abstract::A 69-year-old man became hypocalcemic under medical observation. The hypocalcemia occurred in the presence of circulating immunoreactive parathyroid hormone (PTH). Common causes of secondary hyperparathyroidism were excluded, as was PTH resistance using PTH infusions. The immunoreactive PTH was examined in detail. PTH...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298499
更新日期:1992-08-01 00:00:00
abstract::Vit-D-Dependent calcium binding protein and parvalbumin have both been detected in ameloblasts and calcified cartilage by immunohistochemical techniques. These two Ca2+ binding proteins may play a crucial role in the local accumulation of Ca2+ ions during the process of mineralization. The mechanisms underlying the de...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02405306
更新日期:1984-01-01 00:00:00
abstract::Transcriptional regulation of the postnatal skeleton is incompletely understood. Here, we determined the consequence of loss of early growth response gene 1 (EGR-1) on bone properties. Analyses were performed on both the microscopic and molecular levels utilizing micro-computed tomography (micro-CT) and Fourier transf...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-011-9486-0
更新日期:2011-07-01 00:00:00
abstract::The effect of long-chain n-3 polyunsaturated fatty acid (PUFA) on bone measurements was evaluated in gonad-intact middle-aged male rats. Seven rats were killed on day 0 of dietary intervention to determine bone parameters at baseline. Experimental rats (7/group) were fed one of the following lipid treatments (g/kg die...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-007-9010-8
更新日期:2007-04-01 00:00:00
abstract::Alkaline phosphatase is detected in both soluble and particulate fractions of rat bone. The concentration of alkaline phosphatase in the particulate fraction of rat skull cap bone and femur is high during the peak period of calcification of these bones suggesting the possibility of using it as a marker for the rate of...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555116
更新日期:1986-09-01 00:00:00
abstract::Experimental analysis with paired femurs provides the opportunity to study within-person differences in fracture type and associated structural side differences. We hypothesized that different fracture types in the hips of a subject are associated with structural asymmetry. Bone mineral density (BMD) and structural me...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-010-9388-6
更新日期:2010-09-01 00:00:00
abstract::The native human parathyroid hormone, hPTH-(1-84), and certain carboxyl truncated analogs such as hPTH-(1-34) and even smaller fragments such as hPTH-(1-31)NH2, [Leu27]cyclo(Glu22-Lys26)hPTH-(1-31)NH2, and hPTH-(1-30)NH2 stimulate femoral trabecular and cortical bone growth in ovariectomized (OVX) rats. Here we show t...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010061
更新日期:2000-04-01 00:00:00
abstract::Although several observational studies have demonstrated an association between vitamin K status and bone mineral density (BMD) in postmenopausal women, no placebo-controlled intervention trials of the effect of vitamin K1 supplementation on bone loss have been reported thus far. In the trial presented here we have in...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00223-002-2084-4
更新日期:2003-07-01 00:00:00
abstract::To investigate the effect of low doses of intranasal salmon calcitonin on plasma cyclic AMP (cAMP) and serum ionized calcium, 40 healthy postmenopausal women were randomized to receive a single dose of either placebo or 50, 100, or 200 IU of salmon calcitonin as a nasal spray. Blood samples were collected throughout a...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF02555817
更新日期:1990-01-01 00:00:00
abstract::Results from previous quasi-static mechanical tests indicate that femurs from elderly subjects fail in vitro at forces 50% below those available in a fall from standing height. However, bone is a rate-dependent material, and it is not known whether this imbalance is present at rates of loading which occur in a fall. B...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
doi:10.1007/BF00310169
更新日期:1994-07-01 00:00:00
abstract::Five years ago we reported results from a cross-sectional study of the effect of nutritional factors on calcium-regulating hormones and bone loss in perimenopausal women. We found an inverse correlation between serum 25-hydroxyvitamin D (25OHD) and immunoreactive parathyroid hormone (PTH), and we postulated that over ...
journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
doi:10.1007/BF00334543
更新日期:1992-09-01 00:00:00
abstract::Little is known about hypoxia-induced modification of the canal network in the cortical bone despite its involvement in intracortical vascularity and bone blood supply. In this study, we examined the effect of chronic hypoxia on the canal network in postnatal bone. Tibiae were harvested from 4- and 8-week-old rats (hy...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-010-9415-7
更新日期:2011-01-01 00:00:00
abstract::Inoculation of Moloney murine sarcoma virus (M-MSV) into shank muscles of adult NMRI mice resulted in localized sarcoma and periosteal membrane proliferation with subsequent periosteal bone formation. Newly formed bone arose from outer surface of shank bones. Two weeks after M-MSV inoculation the width of bone cortex ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02409426
更新日期:1981-01-01 00:00:00
abstract::To study the mechanism of action of thyroid hormones on bone, we examined the effects of immunosuppressive and nonimmunosuppressive cyclosporins, as well as of transforming growth factor beta 1 (TGF beta 1), 17 beta-estradiol (E2), and dihydroxytestosterone (DHT) on thyroxine (T4)- and triiodothyronine (T3)-stimulated...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298788
更新日期:1992-02-01 00:00:00
abstract::Noncarious cervical lesions have a multifactorial etiology. Mechanical stress has been identified as one of the factors, but little evidence exists for its cause-effect relationship. This study was conducted at three different pH levels to observe the surface loss on dentin beams under tension and compression. Bovine ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0271-9
更新日期:2006-12-01 00:00:00
abstract::To characterize osteopenic changes in ovariectomized (OVX) rats as a function of time, female Sprague Dawley rats (240 g body weight, 90 days old) were subjected to bilateral ovariectomy or sham surgery and killed at various times from 14-180 days postovariectomy. The proximal tibial metaphysis was processed undecalci...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02571317
更新日期:1988-09-01 00:00:00
abstract::The usefulness of various bone mineral measurement techniques is a subject of current controversy. In order to explore whether disparate conclusions may have arisen from differences in analytic methodology, data from published reports of bone mass and nonviolent fractures have been reanalyzed in terms of fracture risk...
journal_title:Calcified tissue international
pub_type: 杂志文章,meta分析
doi:10.1007/BF02555036
更新日期:1990-03-01 00:00:00
abstract::The potassium pyroantimonate technique was utilized for the selective subcellular localization of calcium in the mandibular condylar cartilage of 1-day-old rats. Electron dense calcium pyroantimonate precipitates were localized principally in mitochondria and at the cell membrane of the chondrocytes. In addition, smal...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02408603
更新日期:1980-01-01 00:00:00
abstract::Syndesmophyte occurrence and axial bone loss were investigated in the heterozygous Tg187 tumor necrosis factor (TNF) transgenic mouse model (Tg-huTNF) of arthritis. Female and male Tg-huTNF mice were compared to wild-type mice (WT) at 2, 4, 6, 8, and 10 weeks. Syndesmophytes, intervertebral disc space, osteoclasts, os...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-017-0237-8
更新日期:2017-06-01 00:00:00
abstract::Vitamin D has long been known to be important for bone health, but there is currently no consensus on the amount of vitamin D needed or how it varies with age, race/ethnicity, body size, absorption efficiency, season, and other factors. This review describes the effects of vitamin D on calcium absorption, parathyroid ...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-012-9606-5
更新日期:2013-02-01 00:00:00
abstract::Tartrate resistant acid phosphatase (TRAP) has been proposed as a new biochemical marker for bone resorption. We have compared this new marker, TRAP, with the classical biochemical markers of bone remodelling, serum alkaline phosphatase (sAP), serum osteocalcin (sBGP), and with the urinary hydroxyproline/creatinine ra...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555896
更新日期:1991-07-01 00:00:00
abstract::We investigated whether polymorphisms in PTHR1 are associated with bone mineral density (BMD), to determine whether the association of this gene with BMD was due to effects on attainment of peak bone mass or effects on subsequent bone loss. The PTHR1 gene, including its 14 exons, their exon-intron boundaries, and 1,50...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-007-9072-7
更新日期:2007-10-01 00:00:00
abstract::The relationship between oral indicators and bone mineral density (BMD) has been studied by many investigators, with mixed and complex results. The purpose of the present cross-sectional study was to evaluate the associations of periodontal conditions and tooth loss with metacarpal BMD (m-BMD) in a community-based coh...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-004-0275-x
更新日期:2005-07-01 00:00:00