Abstract：
:Bone collagen undergoes a series of enzymatic and nonenzymatic posttranslational modifications with maturation. The aim of this study was to analyze the collagenolytic efficiency of cathepsin K in relation to the extent of bone collagen age. Bone collagen posttranslational maturation was induced in vitro by preincubating bovine fetal cortical bone specimens at 37 °C for different times. The collagen enzymatic cross-links pyridinoline (PYD) and deoxypyridinoline (DPD), the advanced glycation end product pentosidine (PEN), and the native (α) and β-isomerized C-telopeptide (CTX) isomers were measured in each bone specimen. After extraction, bone collagen was incubated with human recombinant cathepsin K at different concentrations and its collagenolytic activity was measured by the release of hydroxyproline. To assess the affinity of cathepsin K for isomerized and nonisomerized CTX isomers, incubation with cathepsin K was also performed in the presence of various concentrations of a specific inhibitor. We showed that preincubation of bone collagen at 37 °C induces a marked increase in the bone concentration of PYD, DPD, and PEN and of CTX isomerization as reflected by the ratio of α-/βCTX. This increase was associated with a parallel increase in the efficiency of cathepsin K to solubilize bone collagen. When cathepsin K was incubated in the presence of an inhibitor, the β-isomerized form of collagen from 3-month- and 8-year-old bovine bone was more susceptible to degradation than the native α form. These results suggest that the collagenolytic activity of cathepsin K may be increased toward more matured bone collagen.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Borel O,Gineyts E,Bertholon C,Garnero Pdoi
10.1007/s00223-012-9604-7subject
Has Abstractpub_date
2012-07-01 00:00:00pages
32-9issue
1eissn
0171-967Xissn
1432-0827journal_volume
91pub_type
杂志文章abstract：:Allogenous demineralized bone matrix (DBM) represents a potential scaffold for bone tissue engineering due to its close relation in structure and function with autologous bone, but its supply is often restricted by donor availability. Thus, an expanded source of human bone is needed. The aim of this study was to evalu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9159-9
更新日期：2008-09-01 00:00:00
abstract：:The bone anabolic effect of parathyroid hormone (PTH) therapy is blunted when used in patients who were previously on bisphosphonate treatment. Osteocytes may play a role in the bisphosphonate silencing effect on PTH therapy since bisphosphonates have been shown to reach the lacunocanalicular system. In vivo osteocyte...
journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
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doi：10.1007/s00223-009-9288-9
更新日期：2009-11-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
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更新日期：1991-04-01 00:00:00
abstract：:We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Gr...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-003-0110-9
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0303-5
更新日期：2006-05-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-013-9701-2
更新日期：2013-05-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557681
更新日期：1985-01-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556250
更新日期：1991-11-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005838
更新日期：2000-03-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-012-9662-x
更新日期：2013-01-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296210
更新日期：1992-07-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00614-0
更新日期：2020-02-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02684999
更新日期：2001-01-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0247-9
更新日期：2006-07-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00298606
更新日期：1995-03-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557688
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0464-7
更新日期：2018-12-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00310252
更新日期：1995-09-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02565132
更新日期：1991-08-01 00:00:00
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journal_title：Calcified tissue international
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