Abstract:
:The reactions of free and DNA-bound 2,2,5,5-tetramethylpyrrolidine-N-oxyl (PROXYL) probes with radicals generated during radiolysis of dilute aqueous solutions of DNA were examined. For the free PROXYL probe in deaerated solution with each of the four nucleotides (dAMP, dCMP, dGMP, and TMP) it was found that the pyrimidine radicals were more reactive toward the probe than were the purine radicals. Reactions of the electron adduct of TMP and the hydroxyl radical adducts of dAMP, dGMP, and TMP with the probe resulted in little or no reduction of the probe. For TMP these results are consistent with the fact that both the protonated electron and hydroxyl radical adducts of TMP will covalently bind to the nitroxide function of the probe. Reduction of the PROXYL probe was observed in reactions with the hydroxyl radical adduct of dCMP and with the electron adducts of dAMP, dCMP, and dGMP. Results of the radiolysis of the free PROXYL probe in deaerated dilute solution of DNA suggest that the PROXYL probe protects the DNA from water radical attack as the ratio of DNA bases to PROXYL probe increases above 50:1. Reactions of DNA-bound probes are dependent on the depth of the nitroxide function in relation to the major groove of the DNA helix. Two probes with tether lengths which are less than the depth of the major groove show an expected increase in reactions with DNA base radicals as compared to a probe with a tether that extends beyond the groove. The longer probe is involved largely in reactions with sugar and water radicals along the periphery of the DNA helix. In the presence of oxygen, there is a dramatic decrease in the loss of both the free and DNA-bound probes due to the lack of reaction of these probes with peroxyl radicals formed by the addition of molecular oxygen to DNA radicals.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Swarts SG,Sevilla MDsubject
Has Abstractpub_date
1987-10-01 00:00:00pages
21-35issue
1eissn
0033-7587issn
1938-5404journal_volume
112pub_type
杂志文章abstract::There is a need for minimally invasive biomarkers that can accurately and quickly quantify radiation exposure. Radiation-responsive proteins have applications in clinical medicine and for mass population screenings after a nuclear or radiological incident where the level of radiation exposure and exposure pattern comp...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR13927.1
更新日期:2015-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
abstract::The induction of thymic lymphomas by whole-body X irradiation with four doses of 1.8 Gy (total dose: 7.2 Gy) in C57BL/6 mice was suppressed from a high frequency (90%) to 63% by preirradiation with 0.075 Gy X rays given 6 h before each 1.8-Gy irradiation. This level was further suppressed to 43% by continuous whole-bo...
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doi:10.1667/rr3289
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journal_title:Radiation research
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更新日期:2008-09-01 00:00:00
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journal_title:Radiation research
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更新日期:2005-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr2560.1
更新日期:2011-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14896.1
更新日期:2018-07-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/0033-7587(2001)155[0089:moakem]2.0.co;2
更新日期:2001-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1667/rr2517.1
更新日期:2011-08-01 00:00:00
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journal_title:Radiation research
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更新日期:2012-04-01 00:00:00
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更新日期:2013-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR1952.1
更新日期:2010-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/rr3374
更新日期:2005-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Radiation is an independent risk factor for cardiovascular and cerebrovascular disease in cancer patients. Modern radiotherapy techniques reduce the volume of the heart and major coronary vessels exposed to high doses, but some exposure is often unavoidable. Radiation damage to the myocardium is caused primarily by in...
journal_title:Radiation research
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
abstract::Ketogenic diets are low in carbohydrates and high in fat, which forces cells to rely more heavily upon mitochondrial oxidation of fatty acids for energy. Relative to normal cells, cancer cells are believed to exist under a condition of chronic mitochondrial oxidative stress that is compensated for by increases in gluc...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR14668.1
更新日期:2017-06-01 00:00:00
abstract::Whether inner-shell ionizations of DNA atoms, called core ionizations, are critical events for cell inactivation by ionizing radiations such as 100 keV electrons and gamma rays has been investigated. The number of core ionizations in DNA atoms per gray of the two types of radiations is calculated from various Monte Ca...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0451.1
更新日期:2007-04-01 00:00:00
abstract::Radiation exposure leads to a risk for long-term deterministic and stochastic late effects. Two individuals exposed to protracted photon radiation in the radiological accident at the Lilo Military site in Georgia in 1997 received follow-up treatment and resection of several chronic radiation ulcers in the Bundeswehr H...
journal_title:Radiation research
pub_type: 杂志文章
doi:10.1667/RR0774.1
更新日期:2007-05-01 00:00:00
abstract::Incorporation of bromodeoxyuridine (BrdU) into DNA in both Chinese hamster ovary (CHO) cells and human melanoma (U1) cells reduced the rate of DNA migration in transverse alternating-field electrophoresis (TAFE) agarose gel optimized for separating molecules larger than 2 Mb. This "BrdU migration effect" was independe...
journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00