Abstract：
:Activation of the transcription factor nuclear factor-kappaB (NF-kappaB) is one of the important responses of cells to an external stress such as ionizing radiation. We studied radiation-induced NF-kappaB activation in vivo in male BALB/c mice. After the mice were exposed to 8.5 Gy total-body gamma irradiation, the spleen, mesenteric lymph nodes, thymus, liver, lung, colon, brain and bone marrow were harvested 1, 2.5, 5, 10 and 20 h postirradiation. NF-kappaB DNA-binding activity was analyzed in the nuclear protein extracts by a gel shift assay. When compared to the levels in untreated control mice, radiation induced activation of NF-kappaB in spleen, mesenteric lymph nodes and bone marrow but not in the other tissues examined. In contrast, an i.p. injection of a lethal dose (3 mg/kg) of lipopolysaccharide also increased activity of NF-kappaB in the liver and lung. The gel supershift assay with Nfkb1, Rela and/or Rel antibodies revealed that the specific molecular forms of NF-kappaB activated by radiation in the spleen were Nfkb1 homodimers and Nfkb1/Rela heterodimers. In mesenteric lymph nodes, the heterodimerized Rel/Rela NF-kappaB was also activated. In bone marrow, an NF-kappaB-like binding factor was induced that may be Nfkb1/Rela- and Rel/Rela-like heterodimers, but it exhibited a higher mobility than Nfkb1 homodimers. These results indicate that in vivo, ionizing radiation induces NF-kappaB activation that varies in both tissue distribution and moleoular composition.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Zhou D,Brown SA,Yu T,Chen G,Barve S,Kang BC,Thompson JSsubject
Has Abstractpub_date
1999-06-01 00:00:00pages
703-9issue
6eissn
0033-7587issn
1938-5404journal_volume
151pub_type
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pub_type： 杂志文章
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更新日期：1983-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1985-06-01 00:00:00
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doi：
更新日期：1983-12-01 00:00:00
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更新日期：2000-02-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-09-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
