Abstract：
:Neural stem cells (NSCs) are hitherto regarded as perspective candidates for cell transplantation in clinical therapies for multilevel spinal cord injury and function restoration. However, the extreme drawbacks of NSCs available for injury transplantation still represent a significant bottleneck in neural regeneration medicine. Therefore, it is essential to establish a suitable cell reservoir as an issue-free alternative. Here, we demonstrate that spermatogonial stem cells (SSCs) derived from rat testis robustly give rise to terminally differentiated, functionally mature spinal cord neurons by using an optimized differentiation protocol. After performing a 3-week in vitro differentiation procedure, most cells exhibited neural morphological features and were Tuj-1 positive. Of note, approximately 60 % of the obtained cells coexpressed choline acetyltransferase (CHAT), acetylcholinesterase (AchE), and calcitonin gene-related peptide (CGRP). More importantly, apart from acquisition of neural antigenic and biochemical properties, nearly all neurons efficiently exhibited in vitro functionality similar to wild-type neurons, such as synapse formation, increased neuronal calcium influx, and electrophysiology. This is the first report revealing consistent and reproducible generation of large amounts of functional neurons from SSCs. Collectively, this system is suitable for studies of SSC transdifferentiation into neuronal cells and can provide sufficient neurons for the treatment of spinal cord injury as well as for genetic and small molecule screenings.
journal_name
Mol Neurobioljournal_title
Molecular neurobiologyauthors
Yang H,Liu C,Chen B,An J,Zhang R,Zhang Q,Zhao J,He B,Hao DJdoi
10.1007/s12035-016-0057-2subject
Has Abstractpub_date
2017-01-01 00:00:00pages
788-803issue
1eissn
0893-7648issn
1559-1182pii
10.1007/s12035-016-0057-2journal_volume
54pub_type
杂志文章abstract：:Retrograde signaling is defined as the signaling events leading from the plastids to the nucleus in plants and across the chemical synapse, from the postsynaptic neuron to the presynaptic neuron in animals. The discovery of various retrograde messengers has opened many avenues and clouds of thoughts as to the role of ...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
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journal_title：Molecular neurobiology
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pub_type： 杂志文章,评审
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journal_title：Molecular neurobiology
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journal_title：Molecular neurobiology
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journal_title：Molecular neurobiology
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pub_type： 杂志文章
doi：10.1007/s12035-018-1287-2
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pub_type： 杂志文章,meta分析
doi：10.1007/s12035-016-9823-4
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journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-9740-6
更新日期：2017-03-01 00:00:00
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journal_title：Molecular neurobiology
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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journal_title：Molecular neurobiology
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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更新日期：1989-01-01 00:00:00
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journal_title：Molecular neurobiology
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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journal_title：Molecular neurobiology
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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更新日期：2018-11-01 00:00:00
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journal_title：Molecular neurobiology
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