Abstract：
:B-50 (GAP-43) is an axonal, plasma membrane-associated protein involved in growth cone morphology and function. We have conducted immunocytochemical, electron microscopic, and time-lapse experiments to visualize morphological consequences of local accumulations of B-50 at the plasma membrane of B-50-transfected PC-B2 cells, a clonal PC12 cell line with very low expression of endogenous B-50. The distribution of the transfected B-50 within these cells was inhomogeneous. At sites where the B-50 concentration was locally increased up to twofold, numerous filopodia were present in growth cone-like, substrate-attached regions. When local B-50 concentrations were even higher (up to 6.2-fold), blebs were formed, often containing vesicular structures, heavily decorated with B-50 immunoreactivity. Double labeling with f-actin binding phalloidin revealed that local B-50 accumulations were accompanied by increased actin filament concentrations. Colocalization of B-50 with actin filaments was prominent in filopodia, but was virtually absent in blebs, suggesting a disconnection of the bleb plasma membrane from the actin cytoskeleton. We conclude that B-50 evokes distinct effects on cell-surface activity in PC12 cells depending on its local concentration.
journal_name
Mol Neurobioljournal_title
Molecular neurobiologyauthors
Aarts LH,Verkade P,Schrama LH,Oestreicher AB,Gispen WH,Schotman Pdoi
10.1007/BF02741362subject
Has Abstractpub_date
1999-08-01 00:00:00pages
17-28issue
1eissn
0893-7648issn
1559-1182journal_volume
20pub_type
杂志文章abstract：:Parkinson's disease and dementia with Lewy bodies are major challenges in research and clinical medicine world-wide and contribute to the most common neurodegenerative disorders. Previously, specific mitochondrial polymorphisms have been found to enhance clearance of amyloid-β from the brain of APP-transgenic mice lea...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-015-9399-4
更新日期：2016-09-01 00:00:00
abstract：:Hippocampus-dependent memories, such as social recognition (SRM), are modulated by neurogenesis. However, the precise role of newborn neurons in social memory processing is still unknown. We showed previously that 1 week of enriched environment (EE) is sufficient to increase neurogenesis in the hippocampus (HIP) and t...
journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-9922-2
更新日期：2017-07-01 00:00:00
abstract：:Mammalian brain contains high densities of angiotensin II (Ang II) type 1 (AT1) receptors, localized mainly to specific nuclei within the hypothalamus and brainstem regions. Neuronal AT1 receptors within these areas mediate the stimulatory actions of central Ang II on blood pressure, water and sodium intake, and vasop...
journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
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更新日期：1999-02-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-018-1005-0
更新日期：2018-11-01 00:00:00
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journal_title：Molecular neurobiology
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journal_title：Molecular neurobiology
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更新日期：2015-01-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章,评审
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更新日期：2013-02-01 00:00:00
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journal_title：Molecular neurobiology
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更新日期：2021-02-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-0057-2
更新日期：2017-01-01 00:00:00
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doi：10.1007/s12035-015-9102-9
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-012-8384-4
更新日期：2013-06-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s12035-016-9935-x
更新日期：2017-07-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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更新日期：2018-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
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journal_title：Molecular neurobiology
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12035-019-1625-z
更新日期：2019-11-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1385/MN:22:1-3:041
更新日期：2000-08-01 00:00:00
