Abstract:
:Freeze-tolerant third instar larvae of the gallfly Eurosta solidaginis were cooled at 10, 5, 1, and 0.1 degrees C min-1 to -40 degrees C and then warmed to +5 degrees C at 1 degree C min-1. After cooling and warming the larvae were transferred to 21 degrees C and the survival of larvae, success of pupariation, and adult emergence were monitored at daily intervals in comparison to an uncooled control sample. The percentage emergences of flies from larvae cooled at 10, 5, 1, and 0.1 degree C min-1 and in the control were 7, 13, 37, 77, and 67%, respectively. A number of flies in each group emerged with malformed (unextended) wings and an unretracted ptilinum on the head capsule. The percentage emergences of normal flies at the four cooling rates and from the control were 3, 0, 17, 47, and 57%. At 48 hr after exposure all larvae in each treatment were alive. First mortality was observed between 48 and 72 hr after cooling and increased with time at each cooling rate. Mortality was apportioned into four phases of development: larva, pupariation, and early and late pupae. Mortality commenced earlier at the faster cooling rates; at 10 degrees C min-1, 37% of the sample died as larvae and a further 20% failed to complete pupariation, whereas at 0.1 degree C min-1, only 3% died as larvae and 97% formed a puparium.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Bale JS,Hansen TN,Nishino M,Baust JGdoi
10.1016/0011-2240(89)90024-2subject
Has Abstractpub_date
1989-06-01 00:00:00pages
285-9issue
3eissn
0011-2240issn
1090-2392pii
0011-2240(89)90024-2journal_volume
26pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract::We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2006.05.005
更新日期:2006-10-01 00:00:00
abstract::Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.12.004
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND:The effect of dimethyl sulfoxide (Me2SO) on enumeration of post-thaw CD45+ and CD34+ cells of umbilical cord blood (HPC-C) and mobilized peripheral blood (HPC-A) has not been systematically studied. METHODS:Cells from leukapheresis products from multiple myeloma patients and umbilical cord blood cells were ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.06.003
更新日期:2005-10-01 00:00:00
abstract::Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyo...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.07.012
更新日期:2020-10-01 00:00:00
abstract::Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.01.010
更新日期:2004-06-01 00:00:00
abstract::We have studied the sensitivity to cryoprotecting agents of different embryos of the local sea urchin, Echinometra lucunter which is the species used for embryo-larval bioassays in Brazil. We have located significant differences between both species sensitivity to cryoprotecting agents; while for P. lividus propylene ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2017.12.005
更新日期:2018-02-01 00:00:00
abstract::Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2007.01.001
更新日期:2007-04-01 00:00:00
abstract::Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(03)00017-8
更新日期:2003-04-01 00:00:00
abstract::Dipalmitoylphosphatidylcholine (DPPC) bilayers hydrated in the presence of trehalose were equilibrated at various temperatures (4, 20, and 60 degrees C) corresponding to the crystalline Lc, gel L beta', and liquid-crystalline L alpha phases, respectively, and then desiccated at these temperatures or freeze-dried at -8...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90033-8
更新日期:1988-06-01 00:00:00
abstract::The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.09.010
更新日期:2008-12-01 00:00:00
abstract::Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cry...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.07.008
更新日期:2018-10-01 00:00:00
abstract::In an experimental study using porcine corneas we investigated the influence of several variables of freeze-thaw trauma on survival of corneal endothelial cells after corneal cryopreservation employing chondroitin sulfate as a cryoprotectant. We examined the influence of: (1) the concentration of chondroitin sulfate i...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1993.1039
更新日期:1993-08-01 00:00:00
abstract::This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.01.002
更新日期:2008-04-01 00:00:00
abstract::Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...
journal_title:Cryobiology
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract::Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.03.005
更新日期:2014-06-01 00:00:00
abstract::Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2199
更新日期:1999-09-01 00:00:00
abstract::Boar spermatozoa are extremely sensitive to low temperatures and the cryopreservation causes dramatic changes in sperm survivability, but it is not clear which part of the cryopreservation process affects the most. The aim of this work was to assess early events of apoptotic changes as damage indicators in boar sperm ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.05.012
更新日期:2020-08-01 00:00:00
abstract::The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(03)00045-2
更新日期:2003-06-01 00:00:00
abstract::This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2305
更新日期:2001-02-01 00:00:00
abstract::Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.04.013
更新日期:2018-06-01 00:00:00
abstract::The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.05.002
更新日期:2008-08-01 00:00:00
abstract::We have demonstrated that sugars and suger/zinc mixtures can be used to preserve the activity of dried phosphofructokinase (PFK) during long-term storage over CaSO4. After 9 weeks in the presence of either 200 mM sucrose or 200 mM trehalose little loss of PFK activity was noted, with almost 60% of the original prefree...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90045-4
更新日期:1988-08-01 00:00:00
abstract::The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2013.08.005
更新日期:2013-12-01 00:00:00
abstract::We recently published a protocol to vitrify human articular cartilage and a method of cryoprotectant removal in preparation for transplantation. The current study's goal was to perform a cryoprotectant kinetic analysis and theoretically shorten the procedure used to vitrify human articular cartilage. First, the loadin...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2016.05.007
更新日期:2016-08-01 00:00:00
abstract::The effects of the presence or absence of cumulus cells and equilibration times (1 and 4 min) with cryoprotectant, and Taxol treatment before vitrification (0.5-5.0 microM) on development of in vitro matured porcine oocytes after vitrification were examined. Ethylene glycol (30%) and sucrose (0.5M) was used as a vitri...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.08.002
更新日期:2005-12-01 00:00:00
abstract::The objective of this study was to determine osmotic tolerance of canine spermatozoa. The study comprised three experiments: (1) spermatozoa suspended either in an egg yolk-citrate (EYC) extender or in Kenney skim milk extender were exposed to NaCl solutions ranging from 290 to 1500 mOsm; (2) spermatozoa suspended in ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/S0011-2240(02)00009-3
更新日期:2002-02-01 00:00:00
abstract::The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90009-0
更新日期:1988-04-01 00:00:00
abstract::The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2221
更新日期:2000-02-01 00:00:00
abstract::The effects of rapid cooling of semen (cold shock) from 30 degrees C to various temperatures above 0 degrees C on survival of ram spermatozoa suspended in diluents with or without egg yolk were assessed before and after freezing. Rapid cooling of extended semen from 30 to 15 degrees C had little or no effect on sperma...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(86)90061-1
更新日期:1986-12-01 00:00:00
abstract::Semen was collected in the laboratory from nine healthy donors. The concentrations and the percentages of live and motile spermatozoa in all semen samples were within the normal range. Each sample was diluted with citrate-egg yolk-glycerol medium with and without 5 mM dithiothreitol (DTT). Samples were frozen in liqui...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(84)90052-x
更新日期:1984-10-01 00:00:00