Non-transmission of bacterial and viral microbes to embryos and semen stored in the vapour phase of liquid nitrogen in dry shippers.

abstract：:The cryopreservation of isolated fish blastomeres is likely to provide a valid alternative to embryo cryopreservation, the results of which are still discouraging. A repeatable technique for the cryopreservation of rainbow trout blastomeres has been established and the effect of embryonic developmental stage on freezi...

journal_title：Cryobiology

authors： Calvi SL,Maisse G

更新日期：1998-06-01 00:00:00

abstract：PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...

journal_title：Cryobiology

authors： Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

更新日期：2017-04-01 00:00:00

abstract：:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. ...

journal_title：Cryobiology

authors： Zeng C,Peng W,Ding L,He L,Zhang Y,Fang D,Tang K

更新日期：2014-08-01 00:00:00

abstract：:This study was conducted to evaluate the effects of developmental stage of in vitro produced (IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance. The IVP embryos were vitrified at five different developmental stages: 4-, 8- and 16-cell, morula, and blastocyst. For each stage, half ...

journal_title：Cryobiology

authors： Shirazi A,Soleimani M,Karimi M,Nazari H,Ahmadi E,Heidari B

更新日期：2010-04-01 00:00:00

abstract：:The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...

journal_title：Cryobiology

authors： Yoshimoto T,Nakamura S,Yamauchi S,Muto N,Nakada T,Ashizawa K,Tatemoto H

更新日期：2008-08-01 00:00:00

abstract：:The effect of cryopreservation on ciliary activity of human nasal respiratory epithelium was evaluated. Samples were cryopreserved in a solution containing nutrient medium, 10% fetal calf serum, and two different concentrations (10 or 20%) of dimethyl sulfoxide and stored in liquid nitrogen at -196 degrees C for 2 wee...

journal_title：Cryobiology

authors： Di Benedetto G,Gill J,Lopez-Vidriero MT,Clarke SW

更新日期：1989-08-01 00:00:00

abstract：:We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....

journal_title：Cryobiology

authors： Zeron Y,Tomczak M,Crowe J,Arav A

更新日期：2002-10-01 00:00:00

abstract：:An insect antifreeze protein gene Mpafp698 was cloned by the RT-PCR approach from the desert beetle Microdera punctipennis. The gene was constructed and heterogeneously expressed in Escherichia coli as fusion proteins, His-MpAFP698, glutathione S-transferase (GST)-MpAFP698, and maltose-binding protein (MBP)-MpAFP698. ...

journal_title：Cryobiology

authors： Qiu LM,Ma J,Wang J,Zhang FC,Wang Y

更新日期：2010-04-01 00:00:00

abstract：:The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown is particularly sensitive to cold and readily damaged by exposure to low temperatures, the da...

journal_title：Cryobiology

authors： Suzuki T,Boediono A,Takagi M,Saha S,Sumantri C

更新日期：1996-10-01 00:00:00

abstract：:Cell volumes, viability, ultrastructure, and metabolism have been studied in mouse L cells at reduced water contents. Dehydration was achieved by addition of sorbitol to the incubation medium at concentrations of 0.15, 0.3, and 0.6 m. In 0.3 m sorbitol, cell volume was reduced to about 35% of the initial control level...

journal_title：Cryobiology

authors： Mansell JL,Clegg JS

更新日期：1983-10-01 00:00:00

abstract：:A previous study demonstrated that disaccharides, antioxidants, and caspase inhibitors can be used in freezing solutions to reduce the concentration of Me2SO from the current standard of 10% (v/v) to 5% (v/v) or 2.5% and to eliminate fetal bovine serum (FBS) for the cryopreservation of human amniotic fluid-derived ste...

journal_title：Cryobiology

authors： Cho HJ,Lee SH,Yoo JJ,Shon YH

更新日期：2014-04-01 00:00:00

abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...

journal_title：Cryobiology

authors： Wishnies SM,Parrish AR,Sipes IG,Gandolfi AJ,Putnam CW,Krumdieck CL,Brendel K

更新日期：1991-06-01 00:00:00

abstract：:Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20...

journal_title：Cryobiology

authors： McClean R,Zee YP,Holt WV,Johnston SD

更新日期：2008-12-01 00:00:00

abstract：:Human monocytes purified from peripheral blood by counterflow centrifugal elutriation were cryopreserved in a vitreous state at 1 atm pressure. The vitrification solution was Hanks' balanced salt solution (HBSS) containing (w/v) 20.5% Me2SO, 15.5% acetamide, 10% propylene glycol, and 6% polyethylene glycol. Fifteen mi...

journal_title：Cryobiology

authors： Takahashi T,Hirsh A,Erbe EF,Bross JB,Steere RL,Williams RJ

更新日期：1986-04-01 00:00:00

abstract：:A recent study from our laboratory indicated additional tissue injury during rewarming of a cooled rabbit leg. Oxygen-derived free radicals were believed to play a role in such "rewarming injury." Since free radicals may attack membrane phospholipids, we analyzed the phospholipid composition in the leg tissue during c...

journal_title：Cryobiology

authors： Das DK,Iyengar J,Jones RM,Lu D,Maity S

更新日期：1991-04-01 00:00:00

abstract：:The present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and L...

journal_title：Cryobiology

authors： Kocyigit A,Cevik M

更新日期：2015-08-01 00:00:00

abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...

journal_title：Cryobiology

authors： Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

更新日期：2020-12-04 00:00:00

abstract：PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...

journal_title：Cryobiology

authors： Herbemont C,Mnallah S,Bennani-Smires B,Peigne M,Cedrin-Durnerin I,Grynberg M,Sifer C

更新日期：2021-01-02 00:00:00

abstract：:Toxoplasma gondii is a protozoan parasite that infects birds and mammals, including humans. T. gondii T-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure for T. gondii T-263 bradyzoites has been developed to meet the require...

journal_title：Cryobiology

authors： Booth KS,James ER,Popiel I

更新日期：1996-06-01 00:00:00

abstract：:Sustainability of channel catfish, Ictalurus punctatus ♀ × blue catfish, Ictalurus furcatus ♂ hybrid aquaculture relies on new innovative technologies to maximize fry output. Transplanting spermatogonial stem cells (SSCs) from blue catfish into channel catfish hosts has the potential to greatly increase gamete availab...

journal_title：Cryobiology

authors： Abualreesh M,Myers JN,Gurbatow J,Johnson A,Xing,Wang J,Li S,Coogan M,Vo K,El Husseini N,Dunham RA,Butts IAE

更新日期：2020-12-01 00:00:00

abstract：:The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...

journal_title：Cryobiology

authors： Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

更新日期：2000-06-01 00:00:00

abstract：:Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...

journal_title：Cryobiology

authors： Browne RK,Mahony M,Clulow J

更新日期：2002-06-01 00:00:00

abstract：:Cryopreservation is a technique that has been extensively used for storage of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine. Therefore, improving current cryopreservation procedures in terms of increasing cell viability and functionality is important. In this study, we optimized the cryopreserv...

journal_title：Cryobiology

authors： Lauterboeck L,Hofmann N,Mueller T,Glasmacher B

更新日期：2015-12-01 00:00:00

abstract：:It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thi...

journal_title：Cryobiology

authors： Möhlmann DT

更新日期：2009-06-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...

journal_title：Cryobiology

authors： Liu Y,Xu T,Robinson N,Qin J,Li X

更新日期：2014-04-01 00:00:00

abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...

journal_title：Cryobiology

authors： Cheng LY,Sun TC,Liu XC,Yu H,Zhou SJ,Tian L,Yang SH,Liu BX

更新日期：2020-12-03 00:00:00

abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...

journal_title：Cryobiology

authors： Lewis JG,Learmonth RP,Watson K

更新日期：1994-04-01 00:00:00

abstract：:The purpose of our study was to investigate underlying basic mechanisms of hypothermia-induced cardioprotection during oxidative stress in a cardiomyocyte cell culture model. For hypothermic treatment we cooled H9c2 cardiomyocytes to 20°C, maintained 20min at 20°C during which short-term oxidative damage was inflicted...

journal_title：Cryobiology

authors： Diestel A,Drescher C,Miera O,Berger F,Schmitt KR

更新日期：2011-02-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00