Effects of leukemia inhibitory factor and insulin-like growth factor-I on the cell allocation and cryotolerance of bovine blastocysts.

abstract：:Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (-196 °C). The organic solvent dimethyl sulfoxide (Me2SO) is o...

journal_title：Cryobiology

authors： Solocinski J,Osgood Q,Wang M,Connolly A,Menze MA,Chakraborty N

更新日期：2017-04-01 00:00:00

abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...

journal_title：Cryobiology

authors： Hunter JE,Bernard A,Fuller BJ,McGrath JJ,Shaw RW

更新日期：1992-04-01 00:00:00

abstract：:Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...

journal_title：Cryobiology

authors： Warner RM,Ampo E,Nelson D,Benson JD,Eroglu A,Higgins AZ

更新日期：2020-11-04 00:00:00

abstract：:Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...

journal_title：Cryobiology

authors： Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

更新日期：1995-08-01 00:00:00

abstract：:Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods ...

journal_title：Cryobiology

authors： Bielanski A,Nadin-Davis S,Sapp T,Lutze-Wallace C

更新日期：2000-03-01 00:00:00

abstract：:Two parameters fundamental to cell cryobiology are the water permeability (hydraulic conductivity), Lp, and its activation energy, EA. The Lp can be calculated from two experimental determinations: the critical osmolality, Osmcrit, at which 50% of the cells lyse, and the time, tcrit, to 50% lysis in a highly hyposmoti...

journal_title：Cryobiology

authors： Noiles EE,Thompson KA,Storey BT

更新日期：1997-08-01 00:00:00

abstract：:Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarcti...

journal_title：Cryobiology

authors： Hájek J,Barták M,Hazdrová J,Forbelská M

更新日期：2016-12-01 00:00:00

abstract：:Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cry...

journal_title：Cryobiology

authors： Osei-Bempong C,Ghareeb AE,Lako M,Figueiredo FC,Armitage WJ

更新日期：2018-10-01 00:00:00

abstract：:The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...

journal_title：Cryobiology

authors： Yoshimoto T,Nakamura S,Yamauchi S,Muto N,Nakada T,Ashizawa K,Tatemoto H

更新日期：2008-08-01 00:00:00

abstract：:Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...

journal_title：Cryobiology

authors： Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

更新日期：2019-10-01 00:00:00

abstract：:Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...

journal_title：Cryobiology

authors： Woods EJ,Liu J,Gilmore JA,Reid TJ,Gao DY,Critser JK

更新日期：1999-05-01 00:00:00

abstract：:The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes were centrifuged at 16,000 g for 20 min for the cytoplasmic lipid polarization and their lipid lay...

journal_title：Cryobiology

authors： Murakami M,Otoi T,Sumantri C,Suzuki T

更新日期：1998-05-01 00:00:00

abstract：:Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20...

journal_title：Cryobiology

authors： McClean R,Zee YP,Holt WV,Johnston SD

更新日期：2008-12-01 00:00:00

abstract：AIM:We aim to assess the effect of the state of T cells before cryopreservation on the post-thaw proliferative capacity, phenotype and functional response. METHODS:Peripheral blood mononuclear cells (PBMCs) were isolated from a hepatocellular carcinoma (HCC) patient, and the T cells were frozen during cell culture acc...

journal_title：Cryobiology

authors： Luo Y,Wang P,Liu H,Zhu Z,Li C,Gao Y

更新日期：2017-12-01 00:00:00

abstract：:This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes using the Taguchi method. Freeze-thaw experiments based on the L(8)(2(7)) two-level orthogonal array of the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statisticall...

journal_title：Cryobiology

authors： Lyu SR,Wu WT,Hou CC,Hsieh WH

更新日期：2010-04-01 00:00:00

abstract：:Twenty-eight-day-old male rats were used in three experiments to study whether cold exposure potentiates pineal actions in nonhibernating mammals. The following questions were considered: (a) Can cold exposure increase the antigonadal effects of light deprivation? (b) Are the effects induced by blindness plus cold exp...

journal_title：Cryobiology

authors： Sánchez-Barceló EJ,Cos S,Mediavilla MD

更新日期：1986-02-01 00:00:00

abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

journal_title：Cryobiology

authors： Wusteman M,Boylan S,Pegg DE

更新日期：1996-08-01 00:00:00

abstract：:Among the various types of stem cells, induced pluripotent stem cells (iPSCs) have gained much attention due to their pluripotent nature. iPSCs help us to understand the processes that regulate pluripotency and specialization. However, in order to use them in various applications in regenerative medicine, their effici...

journal_title：Cryobiology

authors： Fernandes S,Khan N,Kale V,Limaye L

更新日期：2019-10-01 00:00:00

abstract：:Cryoablation is a minimally invasive cancer treatment. In this study, the effects of cryoablation on normal rabbit bone were evaluated using imaging and histopathological examinations. Cryoablation was performed using a Cryo-Hit (Galil Medical, Yokneam, Israel). Under anesthesia, one cryoablation needle was inserted a...

journal_title：Cryobiology

authors： Yoshimoto Y,Azuma K,Miya A,Makino E,Nakamoto H,Abe N,Kaburagi M,Ueda H,Kuroda K,Tsuka T,Sugiyama A,Imagawa T,Murahata Y,Itoh N,Osaki T,Shimizu T,Okamoto Y

更新日期：2014-10-01 00:00:00

abstract：:Storing spores is a promising method to conserve genetic diversity of ferns ex situ. Inappropriate water contents or damaging effects of triacylglycerol (TAG) crystallization may cause initial damage and deterioration with time in spores placed at -15 degrees C or liquid nitrogen temperatures. We used differential sca...

journal_title：Cryobiology

authors： Ballesteros D,Walters C

更新日期：2007-08-01 00:00:00

abstract：:Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...

journal_title：Cryobiology

authors： Yu SO,Brown A,Middleton AJ,Tomczak MM,Walker VK,Davies PL

更新日期：2010-12-01 00:00:00

abstract：:Loading with monosaccharide can improve the quality of human red blood cells (hRBCs) frozen with polymer. But in vivo life span of hRBCs frozen with polymer and sugar is not determined. In this study, following incubation with glucose, mouse red blood cells (mRBCs) were frozen in liquid nitrogen for 24h using dextran ...

journal_title：Cryobiology

authors： Quan GB,Liu MX,Han Y,Fang L,Du W,Wang JX

更新日期：2010-08-01 00:00:00

abstract：:Cryopreservation has been reported to damage approximately 40-50% of viable sperm in bull semen. The present study was undertaken to assess the cryo-effectiveness of glycerol (GLY), ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propylene glycol (PND) as cryoprotectant during the cryopreservation of Nguni bull se...

journal_title：Cryobiology

authors： Seshoka MM,Mphaphathi ML,Nedambale TL

更新日期：2016-06-01 00:00:00

abstract：:It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thi...

journal_title：Cryobiology

authors： Möhlmann DT

更新日期：2009-06-01 00:00:00

abstract：:To evaluate the effect of Ca2+ on recovery of heart function after hypothermic preservation, isolated working rat hearts were perfused continuously in different [Ca2+] (0.35-2.0 mM) at 37 degrees C for 30 min, during cooling to 5 degrees C, and then at 5 degrees C for 1, 6 or 18 h. After rewarming to 37 degrees C, con...

journal_title：Cryobiology

authors： Liu B,Lopaschuk GD,Wang LC

更新日期：1994-06-01 00:00:00

abstract：:The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...

journal_title：Cryobiology

authors： Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

更新日期：2000-06-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events...

journal_title：Cryobiology

authors： Rabin Y,Taylor MJ,Feig JS,Baicu S,Chen Z

更新日期：2013-12-01 00:00:00

abstract：:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...

journal_title：Cryobiology

authors： Storey KB

更新日期：2004-04-01 00:00:00

abstract：:This paper describes some new approaches for understanding the permeability of teleost embryos. The dechorionated zebrafish (Brachydanio rerio) was used as a model for basic studies of water and cryoprotectant permeability. These embryos are composed of two compartments, a large yolk (surrounded by the yolk syncytial ...

journal_title：Cryobiology

authors： Hagedorn M,Hsu E,Kleinhans FW,Wildt DE

更新日期：1997-06-01 00:00:00