Abstract：
:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees C. The changes in diameter of oocytes were measured over a 5-min period and a computer model was used to derive values for membrane water permeability (Lp) and inactive volume (Vb) and to compare the experimental data to the predicted values. The results for the mouse oocyte Lp were comparable to values determined by other methods. However the human data, for both failed-to-fertilize and fresh oocytes, have a wide range of values with large standard deviations. The Lp values at the various temperatures were used to calculate the Arrhenius activation energy (Ea). An Ea value of 9.48 kcal/mol was found for the fresh mouse oocyte, whereas the activation energy for human oocytes was extremely low, 3.73 kcal/mol for fresh oocytes and 1.93 kcal/mol for failed-to-fertilize oocytes.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Hunter JE,Bernard A,Fuller BJ,McGrath JJ,Shaw RWdoi
10.1016/0011-2240(92)90022-tsubject
Has Abstractpub_date
1992-04-01 00:00:00pages
240-9issue
2eissn
0011-2240issn
1090-2392pii
0011-2240(92)90022-Tjournal_volume
29pub_type
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