Catalase incorporation in freezing mixture leads to improved recovery of cryopreserved iPSC lines.

Abstract:

:Among the various types of stem cells, induced pluripotent stem cells (iPSCs) have gained much attention due to their pluripotent nature. iPSCs help us to understand the processes that regulate pluripotency and specialization. However, in order to use them in various applications in regenerative medicine, their efficient cryopreservation and recovery after the freezing injury is critical. Here we have used an antioxidant catalase, as an additive to the conventional freezing mixture containing 50% FBS and 10% DMSO. The hiPSCs were frozen as aggregates by using a programmable freezer and then stored in liquid nitrogen at -196 °C. It was seen that catalase improved the revival efficiency by reducing the late apoptotic populations and increasing the live cell fraction. Catalase also retained the pluripotent nature of iPSCs in a better way post revival. This improvement could be attributed to reduction of total ROS and apoptosis, which are the two main factors that cause damage during freezing. Our data suggest that catalase could be a useful additive while freezing hiPSCs.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Fernandes S,Khan N,Kale V,Limaye L

doi

10.1016/j.cryobiol.2019.09.003

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

21-29

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(19)30150-6

journal_volume

90

pub_type

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