Abstract：
:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focused on the freeze-induced changes in gene expression that support freezing survival. Using cDNA library screening, we have documented the freeze-induced up-regulation of a number of genes in wood frogs including both identifiable genes (fibrinogen, ATP/ADP translocase, and mitochondrial inorganic phosphate carrier) and novel proteins (FR10, FR47, and Li16). All three novel proteins share in common the presence of hydrophobic regions that may indicate that they have an association with membranes, but apart from that each shows unique tissue distribution patterns, stimulation by different signal transduction pathways and responses to two of the component stresses of freezing, anoxia, and dehydration. The new application of cDNA array screening technology is opening up a whole new world of possibilities in the search for molecular mechanisms that underlie freezing survival. Array screening of hearts from control versus frozen frogs hints at the up-regulation of adenosine receptor signaling for the possible mediation of metabolic rate suppression, hypoxia inducible factor mediated adjustments of anaerobic metabolism, natriuretic peptide regulation of fluid dynamics, enhanced glucose transporter capacity for cryoprotectant accumulation, defenses against the accumulation of advanced glycation end products, and improved antioxidant defenses as novel parts of natural freeze tolerance that remain to be explored.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Storey KBdoi
10.1016/j.cryobiol.2003.10.008subject
Has Abstractpub_date
2004-04-01 00:00:00pages
134-45issue
2eissn
0011-2240issn
1090-2392pii
S0011224004000070journal_volume
48pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：:This study examines the effectiveness of the cryotop vitrification method for the cryopreservation of goat blastocysts. To determine the effects of embryo development stage and donor age on in vitro survival rates, good-quality blastocysts from adult and prepubertal goats were sorted into non-expanded, expanded, hatch...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2011.09.002
更新日期：2011-12-01 00:00:00
abstract：:Percutaneous ablation is the currently preferred locoregional therapy for non-resectable hepatocellular cancer (HCC). Cryoablation is an attractive option because it forms an ice ball viewable by many imaging methods. This study assessed the therapeutic effect of comprehensive cryoablation (of intra- and extrahepatic ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2012.11.008
更新日期：2013-02-01 00:00:00
abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.10.003
更新日期：2010-04-01 00:00:00
abstract：:Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.05.004
更新日期：2018-08-01 00:00:00
abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(92)90022-t
更新日期：1992-04-01 00:00:00
abstract：:Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.02.007
更新日期：2005-06-01 00:00:00
abstract：:Two parameters fundamental to cell cryobiology are the water permeability (hydraulic conductivity), Lp, and its activation energy, EA. The Lp can be calculated from two experimental determinations: the critical osmolality, Osmcrit, at which 50% of the cells lyse, and the time, tcrit, to 50% lysis in a highly hyposmoti...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1997.2033
更新日期：1997-08-01 00:00:00
abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.cryobiol.2009.05.007. The duplicate article has therefore been withdrawn. ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.05.006
更新日期：2009-06-06 00:00:00
abstract：:Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The pu...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.04.005
更新日期：2014-06-01 00:00:00
abstract：:The present study aimed to examine the behavior of ram spermatozoa subjected to a vitrification process in free-egg yolk diluents in relation with conventional diluents and cryopreservation protocol used in this species. Previously it was investigated the toxicity of cryoprotectants, sucrose and glycerol, based on dif...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.05.004
更新日期：2015-08-01 00:00:00
abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90046-1
更新日期：1989-04-01 00:00:00
abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1996.0042
更新日期：1996-08-01 00:00:00
abstract：:Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2016.10.006
更新日期：2016-12-01 00:00:00
abstract：:Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1995.1036
更新日期：1995-08-01 00:00:00
abstract：:This study was conducted to evaluate the effects of developmental stage of in vitro produced (IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance. The IVP embryos were vitrified at five different developmental stages: 4-, 8- and 16-cell, morula, and blastocyst. For each stage, half ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.11.002
更新日期：2010-04-01 00:00:00
abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2004.08.003
更新日期：2004-12-01 00:00:00
abstract：:Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/s0011-2240(03)00017-8
更新日期：2003-04-01 00:00:00
abstract：:High concentrations of membrane permeable cryoprotectants are necessary to protect human polymorphonuclear leukocytes from osmotic stress injury during freezing, but there are reports that some cryoprotectants are chemically toxic. Cells were exposed to various concentrations of glycerol, dimethyl sulfoxide, or ethyle...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(85)90181-6
更新日期：1985-08-01 00:00:00
abstract：:Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.07.005
更新日期：2020-10-01 00:00:00
abstract：:Cryopreservation of tissue engineered bone (TEB), whilst maintaining its osteogenic ability, is imperative for large-scale clinical application. We previously reported a novel cell transplantation method, in which bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured to confluence and differentiated down th...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.03.011
更新日期：2013-06-01 00:00:00
abstract：:Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...
journal_title：Cryobiology
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1994.1023
更新日期：1994-04-01 00:00:00
abstract：:The susceptibility of in vitro matured (IVM) porcine oocytes to be fertilized in vitro after vitrification was investigated. IVM oocytes were cryopreserved by solid surface vitrification (SSV) or treated with cryoprotectants (toxicity control, TC). Control oocytes were not treated or vitrified. Live oocytes in the thr...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2007.06.008
更新日期：2007-10-01 00:00:00
abstract：:The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.11.007
更新日期：2009-04-01 00:00:00
abstract：:Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.04.013
更新日期：2018-06-01 00:00:00
abstract：:Previous reports established that butylated hydroxy toluene (BHT) minimized cold-induced membrane rupture in sperm from several species. No data regarding the specificity of its effect is available. In this study 25 BHT analogs were tested for their effect on bovine sperm membrane stability. Fourteen were membrane lyt...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(92)90010-y
更新日期：1992-02-01 00:00:00
abstract：:The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.09.010
更新日期：2008-12-01 00:00:00
abstract：:Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2007.01.001
更新日期：2007-04-01 00:00:00
abstract：:Previous studies into the mechanisms governing the freezing of cells in the absence of extracellular ice have been extended to develop a method for the preservation of viable cells in the undercooled state. Deep undercooling of cells is achieved by suspending fine droplets of the cells in oil to make an emulsion, thus...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(85)90030-6
更新日期：1985-12-01 00:00:00
abstract：:We have demonstrated that sugars and suger/zinc mixtures can be used to preserve the activity of dried phosphofructokinase (PFK) during long-term storage over CaSO4. After 9 weeks in the presence of either 200 mM sucrose or 200 mM trehalose little loss of PFK activity was noted, with almost 60% of the original prefree...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(88)90045-4
更新日期：1988-08-01 00:00:00
