Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cytotoxic exposure to glycerol.

abstract：:Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (-196 °C). The organic solvent dimethyl sulfoxide (Me2SO) is o...

journal_title：Cryobiology

authors： Solocinski J,Osgood Q,Wang M,Connolly A,Menze MA,Chakraborty N

更新日期：2017-04-01 00:00:00

abstract：:Among the various types of stem cells, induced pluripotent stem cells (iPSCs) have gained much attention due to their pluripotent nature. iPSCs help us to understand the processes that regulate pluripotency and specialization. However, in order to use them in various applications in regenerative medicine, their effici...

journal_title：Cryobiology

authors： Fernandes S,Khan N,Kale V,Limaye L

更新日期：2019-10-01 00:00:00

abstract：:We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...

journal_title：Cryobiology

authors： Odintsova NA,Boroda AV,Maiorova MA,Yakovlev KV

更新日期：2017-08-01 00:00:00

abstract：:Renal epithelial cells from donor kidneys are susceptible to hypothermic preservation injury, which is attenuated when they over express the cytoskeletal linker protein ezrin. This study was designed to characterize the mechanisms of this protection. Renal epithelial cell lines were created from LLC-PK1 cells, which e...

journal_title：Cryobiology

authors： Tian T,Lindell SL,Lam M,Mangino MJ

更新日期：2012-08-01 00:00:00

abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...

journal_title：Cryobiology

authors： Edashige K,Asano A,An TZ,Kasai M

更新日期：1999-06-01 00:00:00

abstract：BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...

journal_title：Cryobiology

authors： Solanes N,Rigol M,Khabiri E,Castellà M,Ramírez J,Roqué M,Agustí E,Roig E,Pérez-Villa F,Segalés J,Pomar JL,Engel P,Massaguer A,Martorell J,Rodríguez JA,Sanz G,Heras M

更新日期：2005-10-01 00:00:00

abstract：PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...

journal_title：Cryobiology

authors： Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

更新日期：2017-04-01 00:00:00

abstract：:The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...

journal_title：Cryobiology

authors： Song WJ,Jiji LM

更新日期：1988-04-01 00:00:00

abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...

journal_title：Cryobiology

authors： De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

更新日期：1998-09-01 00:00:00

abstract：:The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ros...

journal_title：Cryobiology

authors： Orekhova A,Barták M,Hájek J

更新日期：2018-12-01 00:00:00

abstract：:The effects of rapid cooling of semen (cold shock) from 30 degrees C to various temperatures above 0 degrees C on survival of ram spermatozoa suspended in diluents with or without egg yolk were assessed before and after freezing. Rapid cooling of extended semen from 30 to 15 degrees C had little or no effect on sperma...

journal_title：Cryobiology

authors： Fiser PS,Fairfull RW

更新日期：1986-12-01 00:00:00

abstract：:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34...

journal_title：Cryobiology

authors： Miura J,Minegishi M,Itoh T,Kitaura T,Fukawa N,Takahashi H,Suzuki A,Kudo Y,Narita A,Sato Y,Suzuki M,Wada Y,Takeyama Y,Watanabe T,Tsuchiya S

更新日期：2008-10-01 00:00:00

abstract：:The common carotid artery of the rabbit, a typical small elastic artery, can be cryopreserved using dimethyl sulfoxide, slow cooling, storage at less than -160 degrees C, and rapid warming. This technique provides satisfactory preservation of muscle and endothelial cells, but in about 75% of cases, gross circumferenti...

journal_title：Cryobiology

authors： Hunt CJ,Song YC,Bateson EA,Pegg DE

更新日期：1994-10-01 00:00:00

abstract：:(1) Tyrosine and tryptophan metabolism in brain and peripheral tissues were studied in hypothermic hibernating and normothermic nonhibernating 13-lined ground squirrels (Spermophilus tridecemlineatus). (2) In the hypothermic hibernating state, there were significant elevations of brain stem tyrosine, norepinephrine, a...

journal_title：Cryobiology

authors： Weekley BL,Harlow HJ

更新日期：1987-12-01 00:00:00

abstract：:The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...

journal_title：Cryobiology

authors： Acker JP,McGann LE

更新日期：2000-02-01 00:00:00

abstract：:A significant proportion of couples at reproductive age rely on assisted reproductive technology to overcome infertility. In vitro fertilisation (IVF) involves typically the use of exogenous gonadotropins to stimulate the ovary to produce oocytes, which are collected surgically. After fertilization by conventional IVF...

journal_title：Cryobiology

authors： Sciorio R,Esteves SC

更新日期：2020-02-01 00:00:00

abstract：:Reperfusion injury has been suggested to cause delayed graft function in renal transplantation. Methods to reduce reperfusion injury could lead to improved clinical renal transplantation. Glycine has been shown to suppress reperfusion injury in rabbit renal tubules and rat hepatocytes. In this study we have determined...

journal_title：Cryobiology

authors： Saunder A,Ametani MS,Belzer FO,Southard JH

更新日期：1993-06-01 00:00:00

abstract：:The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...

journal_title：Cryobiology

authors： Arnaud FG,Khirabadi B,Fahy GM

更新日期：2003-06-01 00:00:00

abstract：:Cold storage (at 4°C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and ...

journal_title：Cryobiology

authors： Puts CF,Berendsen TA,Bruinsma BG,Ozer S,Luitje M,Usta OB,Yarmush ML,Uygun K

更新日期：2015-08-01 00:00:00

abstract：:Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver p...

journal_title：Cryobiology

authors： Knecht C,Balaban CL,Rodríguez JV,Ceccarelli EA,Guibert EE,Rosano GL

更新日期：2018-12-01 00:00:00

abstract：:Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...

journal_title：Cryobiology

authors： Zhang T,Isayeva A,Adams SL,Rawson DM

更新日期：2005-06-01 00:00:00

abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...

journal_title：Cryobiology

authors： Robles V,Cabrita E,Real M,Alvarez R,Herráez MP

更新日期：2003-08-01 00:00:00

abstract：:Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...

journal_title：Cryobiology

authors： Anil S,Rawson D,Zhang T

更新日期：2018-08-01 00:00:00

abstract：:This study analysed the effects of cryopreservation on periodontal regeneration of autotransplanted rat molars. First and second maxillary molars (n=92) of 24 four-week-old Wistar rats were gently extracted and autotransplanted into the abdominal tissue immediately (control group n=44) or after cryopreservation in liq...

journal_title：Cryobiology

authors： Staels S,De Coster P,Vral A,Temmerman L,De Pauw G

更新日期：2013-06-01 00:00:00

abstract：:Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...

journal_title：Cryobiology

authors： Bielanski A

更新日期：2005-04-01 00:00:00

abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

journal_title：Cryobiology

authors： Salmon VM,Leclerc P,Bailey JL

更新日期：2017-02-01 00:00:00

abstract：:Loading with monosaccharide can improve the quality of human red blood cells (hRBCs) frozen with polymer. But in vivo life span of hRBCs frozen with polymer and sugar is not determined. In this study, following incubation with glucose, mouse red blood cells (mRBCs) were frozen in liquid nitrogen for 24h using dextran ...

journal_title：Cryobiology

authors： Quan GB,Liu MX,Han Y,Fang L,Du W,Wang JX

更新日期：2010-08-01 00:00:00

abstract：:Collagenase-isolated islets, which had been cultured for 1 week, were frozen at two different cooling rates. Islets frozen at 5 degrees C/min behaved from a functional point of view very similarly to that of nonfrozen, cultured control islets, except for a reduced maximal insulin secretory capacity and a reduced insul...

journal_title：Cryobiology

authors： Andersson A,Sandler S

更新日期：1983-04-01 00:00:00

abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

journal_title：Cryobiology

authors： Mazur P,Schneider U,Mahowald AP

更新日期：1992-02-01 00:00:00