Peripheral tissue freezing in cryosurgery.

abstract：:The field of stem-cell biology has emerged as a key technology for the treatment of various disorders and tissue regeneration applications. However, a major problem remains in clinical practice, which is the question of whether stem cells preserve their self-renewal and differentiation potential in the culture conditi...

journal_title：Cryobiology

authors： Demirci S,Doğan A,Şişli B,Sahin F

更新日期：2014-02-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

journal_title：Cryobiology

authors： Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

更新日期：1989-04-01 00:00:00

abstract：:The [K+]/[Na+] ratio of rabbit renal cortical slices was used to examine, at 25 degrees C, the effects on viability of three cryoprotectant agents (CPA) (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) in combination with three vehicle solutions (Krebs-Henseleit (K-H), solution A, and RPS-2). Viability asse...

journal_title：Cryobiology

authors： Clark P,Fahy GM,Karow AM Jr

更新日期：1984-06-01 00:00:00

abstract：:Sustainability of channel catfish, Ictalurus punctatus ♀ × blue catfish, Ictalurus furcatus ♂ hybrid aquaculture relies on new innovative technologies to maximize fry output. Transplanting spermatogonial stem cells (SSCs) from blue catfish into channel catfish hosts has the potential to greatly increase gamete availab...

journal_title：Cryobiology

authors： Abualreesh M,Myers JN,Gurbatow J,Johnson A,Xing,Wang J,Li S,Coogan M,Vo K,El Husseini N,Dunham RA,Butts IAE

更新日期：2020-12-01 00:00:00

abstract：:A lipoprotein-like antifreeze protein (type IV AFP) has previously been isolated only from the blood plasma of the longhorn sculpin. However, the plasma antifreeze activity in all individuals of this species tested from Newfoundland and New Brunswick waters ranges from low to undetectable. A close relative of the long...

journal_title：Cryobiology

authors： Gauthier SY,Scotter AJ,Lin FH,Baardsnes J,Fletcher GL,Davies PL

更新日期：2008-12-01 00:00:00

abstract：:Cell volumes, viability, ultrastructure, and metabolism have been studied in mouse L cells at reduced water contents. Dehydration was achieved by addition of sorbitol to the incubation medium at concentrations of 0.15, 0.3, and 0.6 m. In 0.3 m sorbitol, cell volume was reduced to about 35% of the initial control level...

journal_title：Cryobiology

authors： Mansell JL,Clegg JS

更新日期：1983-10-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00

abstract：:Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

journal_title：Cryobiology

authors： Karow AM Jr,Jeske AH

更新日期：1976-08-01 00:00:00

abstract：:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

journal_title：Cryobiology

authors： Hao YJ,Liu QL,Deng XX

更新日期：2001-08-01 00:00:00

abstract：:The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...

journal_title：Cryobiology

authors： Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

更新日期：2006-02-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:Intracerebral grafting of immature brain tissue is now widely used as a tool to study neuronal development and regeneration in the brain and spinal cord. This has stimulated the interest in methods for storage of such tissue before transplantation. In this study a method for cryopreservation of immature rat central ne...

journal_title：Cryobiology

authors： Jensen S,Sørensen T,Zimmer J

更新日期：1987-04-01 00:00:00

abstract：:Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...

journal_title：Cryobiology

authors： Bielanski A

更新日期：2005-04-01 00:00:00

abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

journal_title：Cryobiology

authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

更新日期：2020-11-26 00:00:00

abstract：:We have shown previously that the survival of human red blood cells during slow freezing at 2% hematocrit is dependent more on the magnitude of the unfrozen fraction than on the salt concentration in that unfrozen fraction. In parallel, first Nei and more recently Pegg and colleagues have shown that survival is affect...

journal_title：Cryobiology

authors： Mazur P,Cole KW

更新日期：1985-12-01 00:00:00

abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...

journal_title：Cryobiology

authors： Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

更新日期：2020-12-04 00:00:00

abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...

journal_title：Cryobiology

authors： Lewis JG,Learmonth RP,Watson K

更新日期：1994-04-01 00:00:00

abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...

journal_title：Cryobiology

authors： Wishnies SM,Parrish AR,Sipes IG,Gandolfi AJ,Putnam CW,Krumdieck CL,Brendel K

更新日期：1991-06-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:All the aqueous solutions of linear saturated polyalcohols with four carbons have been investigated at low temperature. Only ice has been observed in the solutions of 1,3-butanediol and 1,2,3- and 1,2,4-butanetriol. For same solute concentration, the glass-forming tendency on cooling is highest with 2,3-butanediol, wh...

journal_title：Cryobiology

authors： Boutron P,Mehl P,Kaufmann A,Angibaud P

更新日期：1986-10-01 00:00:00

abstract：:Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects ...

journal_title：Cryobiology

authors： Osipova VP,Berberova NT,Gazzaeva RA,Kudryavtsev KV

更新日期：2016-04-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. This study on the Anuran amphibian (B...

journal_title：Cryobiology

authors： Browne RK,Clulow J,Mahony M,Clark A

更新日期：1998-12-01 00:00:00

abstract：:(1) Tyrosine and tryptophan metabolism in brain and peripheral tissues were studied in hypothermic hibernating and normothermic nonhibernating 13-lined ground squirrels (Spermophilus tridecemlineatus). (2) In the hypothermic hibernating state, there were significant elevations of brain stem tyrosine, norepinephrine, a...

journal_title：Cryobiology

authors： Weekley BL,Harlow HJ

更新日期：1987-12-01 00:00:00

abstract：OBJECTIVE:Liver metastases occur in approximately 4%-14% of gastric cancer patients and are associated with high mortality. However, no standardized treatment approach is available for these patients. We aimed to assess the clinical outcomes of patients with gastric cancer liver metastases (GCLM) who underwent percutan...

journal_title：Cryobiology

authors： Chang X,Wang Y,Yu HP,Zhang WH,Yang XL,Guo Z

更新日期：2018-06-01 00:00:00

abstract：:The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown is particularly sensitive to cold and readily damaged by exposure to low temperatures, the da...

journal_title：Cryobiology

authors： Suzuki T,Boediono A,Takagi M,Saha S,Sumantri C

更新日期：1996-10-01 00:00:00

abstract：BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...

journal_title：Cryobiology

authors： Solanes N,Rigol M,Khabiri E,Castellà M,Ramírez J,Roqué M,Agustí E,Roig E,Pérez-Villa F,Segalés J,Pomar JL,Engel P,Massaguer A,Martorell J,Rodríguez JA,Sanz G,Heras M

更新日期：2005-10-01 00:00:00

abstract：UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...

journal_title：Cryobiology

authors： Duvivier V,Darquy S,Reach G

更新日期：1999-06-01 00:00:00

abstract：:The cryopreservation of isolated fish blastomeres is likely to provide a valid alternative to embryo cryopreservation, the results of which are still discouraging. A repeatable technique for the cryopreservation of rainbow trout blastomeres has been established and the effect of embryonic developmental stage on freezi...

journal_title：Cryobiology

authors： Calvi SL,Maisse G

更新日期：1998-06-01 00:00:00

abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

journal_title：Cryobiology

authors： Bagia JS,Perera DS,Morris DL

更新日期：1998-06-01 00:00:00