Viability tests of cryopreserved endocrine pancreatic cells.

abstract：:Hepatic cryotherapy is increasingly used in the treatment of patients with multiple hepatic metastases, particularly from colorectal cancer. The Cryotech LCS 2000 system, with insulated shaft-circulated liquid nitrogen probes, was designed for this purpose and was evaluated on the bench and in an animal model. The 9-m...

journal_title：Cryobiology

authors： Dilley AV,Dy DY,Warlters A,Copeland S,Gillies AE,Morris RW,Gibb DB,Cook TA,Morris DL

更新日期：1993-02-01 00:00:00

abstract：:Trehalose, a non-reducing glucose disaccharide found at high concentrations in many species of anhydrobiotic organisms, shows significant promise in protecting cellular viability and structural integrity during freezing and desiccation. As mammalian cell membranes are impermeable to trehalose, extensive efforts have b...

journal_title：Cryobiology

authors： Holovati JL,Acker JP

更新日期：2007-10-01 00:00:00

abstract：:This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes using the Taguchi method. Freeze-thaw experiments based on the L(8)(2(7)) two-level orthogonal array of the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statisticall...

journal_title：Cryobiology

authors： Lyu SR,Wu WT,Hou CC,Hsieh WH

更新日期：2010-04-01 00:00:00

abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...

journal_title：Cryobiology

authors： Lewis JG,Learmonth RP,Watson K

更新日期：1994-04-01 00:00:00

abstract：:1,3-Butanediol and 2,3-butanediol have been used in buffered solutions with 20, 30, or 35% (w/w) alcohol to cool erythrocytes to -196 degrees C at different cooling rates between 1 to 3500 degrees C/min, followed by slow or rapid rewarming. 1,3-butanediol shows the same shapes of red blood cell survival curves as 1,2-...

journal_title：Cryobiology

authors： Mehl P,Boutron P

更新日期：1988-02-01 00:00:00

abstract：:Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...

journal_title：Cryobiology

authors： Choi JK,Huang H,He X

更新日期：2015-06-01 00:00:00

abstract：:This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...

journal_title：Cryobiology

authors： Zhang T,Rawson DM,Tosti L,Carnevali O

更新日期：2008-04-01 00:00:00

abstract：:Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 ...

journal_title：Cryobiology

authors： Hashimoto M,Schmid HA,Ohwatari N,Pleschka K

更新日期：1998-11-01 00:00:00

abstract：:Malignant mesothelioma (MM) is an aggressive neoplasm usually arising from the mesothelial surfaces of the pleural or peritoneal cavity. Currently, no standard therapy is available. The most commonly used therapy is cytoreductive surgery combined with systematic chemotherapy, but the median overall survival (OS) is le...

journal_title：Cryobiology

authors： Chen J,Liang B,Yuan Y,Liu C,Li L,Li H,Mu F,Zuo J,Xu K

更新日期：2012-12-01 00:00:00

abstract：:Toxoplasma gondii is a protozoan parasite that infects birds and mammals, including humans. T. gondii T-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure for T. gondii T-263 bradyzoites has been developed to meet the require...

journal_title：Cryobiology

authors： Booth KS,James ER,Popiel I

更新日期：1996-06-01 00:00:00

abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

journal_title：Cryobiology

authors： Biggar KK,Dubuc A,Storey K

更新日期：2009-12-01 00:00:00

abstract：:Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two ho...

journal_title：Cryobiology

authors： Nath J,Smith TB,Patel K,Ebbs SR,Hollis A,Tennant DA,Ludwig C,Ready AR

更新日期：2017-02-01 00:00:00

abstract：:Cell injury due to osmotic dehydration, which is regarded as a major cause of injury during freeze-thaw processes, was examined closely using a perfusion microscope. Human prostatic adenocarcinoma cells (PC-3), which were put in a chamber, were subjected to hyperosmotic stresses by perfusing NaCl solutions of varying ...

journal_title：Cryobiology

authors： Zawlodzka S,Takamatsu H

更新日期：2005-02-01 00:00:00

abstract：:We investigated cryopreservation of horizontal transmission stages of Holospora undulata, a micronucleus-specific bacterial parasite of Paramecium caudatum. Unlike in previous studies on related Holospora species, protocols using glycerol as cryoprotectant failed entirely. In contrast, freezing with dimethyl sulfoxide...

journal_title：Cryobiology

authors： Millot L,Kaltz O

更新日期：2006-02-01 00:00:00

abstract：:In cryopreservation procedures, the capacity to protect the cells from freezing and thawing processes is sensitive to the choice of the cryoprotective agent (CPA) and to its optimal concentration. The advancement of research on Tunicate model species has raised interest in liquid nitrogen cryopreservation for the stor...

journal_title：Cryobiology

authors： Sorrenti G,Bagnoli A,Miraglia V,Crocetta F,Vitiello V,Ristoratore F,Cirino P,Sansone G,Sordino P

更新日期：2014-02-01 00:00:00

abstract：:Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...

journal_title：Cryobiology

authors： Shaw JM,Trounson AO

更新日期：1989-10-01 00:00:00

abstract：:Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...

journal_title：Cryobiology

authors： Jiongyuan X,Lizhi N,Feng M,Shupeng L,Yin L,Mengtian L,Jianying Z,Fei Y,Jibing C,Jialiang L,Kecheng X

更新日期：2013-12-01 00:00:00

abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

journal_title：Cryobiology

authors： Salmon VM,Leclerc P,Bailey JL

更新日期：2017-02-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00

abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

journal_title：Cryobiology

authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

更新日期：1997-11-01 00:00:00

abstract：:Trypsin activity oscillations are shown by the autocatalytic activation of trypsinogen at 0 degrees C in aqueous solution. The oscillations were observed for 3-4 days and show only slight decrease in enzyme activity. The zymogen has been kept at ice water temperature and pH 8.2 in the presence of Mn2+ ion. The mean pe...

journal_title：Cryobiology

authors： Vajda T

更新日期：1986-06-01 00:00:00

abstract：:Bone spavin, splint, and fractured splint bone injuries have been treated with varying methodologies at Wheatley Hall Farm Equine Clinic. Cryosurgery is the most successful. With cryosurgery the small, pain-producing afferent C fibers are destroyed, and painful neuromas do not return. Injured sites were cryosurgically...

journal_title：Cryobiology

authors： McKibbin LS,Paraschak DM

更新日期：1985-10-01 00:00:00

abstract：:Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects ...

journal_title：Cryobiology

authors： Osipova VP,Berberova NT,Gazzaeva RA,Kudryavtsev KV

更新日期：2016-04-01 00:00:00

abstract：:Human monocytes purified from peripheral blood by counterflow centrifugal elutriation were cryopreserved in a vitreous state at 1 atm pressure. The vitrification solution was Hanks' balanced salt solution (HBSS) containing (w/v) 20.5% Me2SO, 15.5% acetamide, 10% propylene glycol, and 6% polyethylene glycol. Fifteen mi...

journal_title：Cryobiology

authors： Takahashi T,Hirsh A,Erbe EF,Bross JB,Steere RL,Williams RJ

更新日期：1986-04-01 00:00:00

abstract：:During cryopreservation, spermatozoa are exposed to chemical or physical stress that has adverse effects on the quality of mammalian spermatozoa. Recently, much attention has been paid to environmental contaminants (ECs) in livestock, because of their detrimental effects on livestock productivity and fertility. ECs li...

journal_title：Cryobiology

authors： Naderi N,Hajian M,Souri M,Nasr Esfahani MH,Vash NT

更新日期：2021-01-16 00:00:00

abstract：:Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...

journal_title：Cryobiology

authors： Soule E,Bandyk M,Matteo J

更新日期：2018-06-01 00:00:00

abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

journal_title：Cryobiology

authors： Bagia JS,Perera DS,Morris DL

更新日期：1998-06-01 00:00:00

abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

journal_title：Cryobiology

authors： Wang HS,Kang L

更新日期：2005-10-01 00:00:00

abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

journal_title：Cryobiology

authors： Mazur P,Schneider U,Mahowald AP

更新日期：1992-02-01 00:00:00

abstract：:All the aqueous solutions of linear saturated polyalcohols with four carbons have been investigated at low temperature. Only ice has been observed in the solutions of 1,3-butanediol and 1,2,3- and 1,2,4-butanetriol. For same solute concentration, the glass-forming tendency on cooling is highest with 2,3-butanediol, wh...

journal_title：Cryobiology

authors： Boutron P,Mehl P,Kaufmann A,Angibaud P

更新日期：1986-10-01 00:00:00