Abstract:
:Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 and 57% of spontaneously active neurons in the medial preoptic area were classified as warm-sensitive in rats and hamsters, respectively. With decreasing slice temperature, the number of active neurons decreased progressively without a significant difference between rats and hamsters. At a slice temperature of 10 degreesC, 57% of all hypothalamic neurons in rats and 42% in hamsters were still spontaneously active. The average temperature at which activity ceased completely when the temperature was decreased further (the cut-off temperature) was 7.9 +/- 0.3 degreesC (n = 14) in rats but was significantly lower at 4.9 +/- 0.4 degreesC (n = 8) in hamsters (P < 0.001). Firing rates and temperature coefficients did not differ in their temperature dependence between rats and hamsters. Action potential duration increased with decreasing slice temperature in both species, but the increase in duration was significantly greater in rats.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Hashimoto M,Schmid HA,Ohwatari N,Pleschka Kdoi
10.1006/cryo.1998.2122subject
Has Abstractpub_date
1998-11-01 00:00:00pages
254-62issue
3eissn
0011-2240issn
1090-2392pii
S0011-2240(98)92122-8journal_volume
37pub_type
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