Cryoprotection of yeast by alcohols during rapid freezing.


:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol proved superior to all other cryoprotectants tested, including glycerol, dimethyl sulfoxide, lactose, trehalose, polyethylene glycol, and polyvinyl pyrrolidone. Propan-2-ol also demonstrated a small but measurable effect although propan-1-ol and butan-1-ol demonstrated no cryoprotective effect. A minimum cooling rate of 25 degrees C min-1 to elicit the cryoprotective effect of ethanol was necessary; below this rate it acted as a cryosensitizer. At cooling rates up to 650 degrees C min-1 substantial cryoprotective effect was still evident. Although the effect of ethanol was variable for other yeast genera tested, ethanol acted positively for all strains of S. cerevisiae. We hypothesize that the cryoprotective effect of alcohols during rapid cooling is a result of their ability to induce increased membrane permeability, allowing rapid water equilibration during extracellular freezing and avoidance of intracellular ice crystal formation.






Lewis JG,Learmonth RP,Watson K




Has Abstract


1994-04-01 00:00:00














  • Corneal cryopreservation with chondroitin sulfate.

    abstract::In an experimental study using porcine corneas we investigated the influence of several variables of freeze-thaw trauma on survival of corneal endothelial cells after corneal cryopreservation employing chondroitin sulfate as a cryoprotectant. We examined the influence of: (1) the concentration of chondroitin sulfate i...


    pub_type: 杂志文章


    authors: Hagenah M,Böhnke M

    更新日期:1993-08-01 00:00:00

  • Effects of cryopreservation on the immunogenicity of porcine arterial allografts in early stages of transplant vasculopathy.

    abstract:BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...


    pub_type: 杂志文章


    authors: Solanes N,Rigol M,Khabiri E,Castellà M,Ramírez J,Roqué M,Agustí E,Roig E,Pérez-Villa F,Segalés J,Pomar JL,Engel P,Massaguer A,Martorell J,Rodríguez JA,Sanz G,Heras M

    更新日期:2005-10-01 00:00:00

  • Effects of cell concentration on viability and metabolic activity during cryopreservation.

    abstract::Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...


    pub_type: 杂志文章


    authors: De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

    更新日期:1998-09-01 00:00:00

  • Current techniques and the future of lung preservation.

    abstract::Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...


    pub_type: 杂志文章,评审


    authors: Lautner LJ,Freed DH,Nagendran J,Acker JP

    更新日期:2020-06-01 00:00:00

  • Trehalose uptake through P2X7 purinergic channels provides dehydration protection.

    abstract::The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...


    pub_type: 杂志文章


    authors: Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

    更新日期:2006-02-01 00:00:00

  • Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing.

    abstract::Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...


    pub_type: 杂志文章


    authors: Hunt CJ,Armitage SE,Pegg DE

    更新日期:2003-02-01 00:00:00

  • Cardiovascular and autonomic responses to whole-body cryostimulation in essential hypertension.

    abstract::Over recent years, a considerable increase in the popularity of cryostimulation and whole body cryotherapy (WBC) procedures has occurred both among healthy individuals and in various groups of patients, including those with primary untreated hypertension. The aim of this study was to compare the effects of WBC on the ...


    pub_type: 杂志文章


    authors: Zalewski P,Buszko K,Zawadka-Kunikowska M,Słomko J,Szrajda J,Klawe JJ,Tafil-Klawe M,Sinski M,Newton J

    更新日期:2014-10-01 00:00:00

  • Effects of kinetin supplementation on the post-thaw motility, viability, and structural integrity of dog sperm.

    abstract::Oxidative stress is one of the major issues associated with cryopreservation because it causes a marked reduction in the post-thaw quality of semen. This study investigated the ability of kinetin to preserve the structural and functional integrity of dog sperm during cryopreservation. Pooled ejaculates were divided in...


    pub_type: 杂志文章


    authors: Qamar AY,Fang X,Bang S,Kim MJ,Cho J

    更新日期:2020-08-01 00:00:00

  • Simplified cryopreservation of porcine cloned blastocysts.

    abstract::Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...


    pub_type: 杂志文章


    authors: Du Y,Zhang Y,Li J,Kragh PM,Kuwayama M,Ieda S,Zhang X,Schmidt M,Bøgh IB,Purup S,Pedersen AM,Villemoes K,Yang H,Bolund L,Vajta G

    更新日期:2007-04-01 00:00:00

  • Galacto-oligosaccharides as protective molecules in the preservation of Lactobacillus delbrueckii subsp. bulgaricus.

    abstract::In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...


    pub_type: 杂志文章


    authors: Tymczyszyn EE,Gerbino E,Illanes A,Gómez-Zavaglia A

    更新日期:2011-04-01 00:00:00

  • Non-disaccharide-based mechanisms of protection during drying.

    abstract::Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of dis...


    pub_type: 杂志文章,评审


    authors: Oliver AE,Leprince O,Wolkers WF,Hincha DK,Heyer AG,Crowe JH

    更新日期:2001-09-01 00:00:00

  • Hepatic cold hypoxia and oxidative stress: implications for ICAM-1 expression and modulation by glutathione during experimental isolated liver preservation.

    abstract::Cold preservation and reperfusion of liver during transplantation are necessary steps in the procedure but which are also associated with damage to the organ. One aspect of this damage is thought to concern up-regulation of inflammatory markers, such as the adhesion molecule intercellular adhesion molecule 1 (ICAM-1) ...


    pub_type: 杂志文章


    authors: El-Wahsh M,Fuller B,Davidson B,Rolles K

    更新日期:2003-10-01 00:00:00

  • Improved stability of live attenuated vaccine gdhA derivative Pasteurella multocida B:2 by freeze drying method for use as animal vaccine.

    abstract::The efficacy of attenuated strain of gdhA derivative Pasteurella multocida B:2 mutant as a live vaccine to control haemorrhagic septicaemia (HS) disease in cattle and buffaloes has been demonstrated. In order to use P. multocida B:2 mutant as a commercial product, it is essential to optimise its formulation for high v...


    pub_type: 杂志文章


    authors: Oslan SNH,Halim M,Ramle NA,Saad MZ,Tan JS,Kapri MR,Ariff AB

    更新日期:2017-12-01 00:00:00

  • Improved rat heart function after low Ca2+ perfused preservation at 5 degrees C for 18 hours.

    abstract::To evaluate the effect of Ca2+ on recovery of heart function after hypothermic preservation, isolated working rat hearts were perfused continuously in different [Ca2+] (0.35-2.0 mM) at 37 degrees C for 30 min, during cooling to 5 degrees C, and then at 5 degrees C for 1, 6 or 18 h. After rewarming to 37 degrees C, con...


    pub_type: 杂志文章


    authors: Liu B,Lopaschuk GD,Wang LC

    更新日期:1994-06-01 00:00:00

  • The effect of cooling rate and temperature on the toxicity of ethylene glycol in the rabbit internal carotid artery.

    abstract::The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...


    pub_type: 杂志文章


    authors: Wusteman M,Boylan S,Pegg DE

    更新日期:1996-08-01 00:00:00

  • Effects of different cryoprotectants on the viability and biological characteristics of porcine preadipocyte.

    abstract::Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...


    pub_type: 杂志文章


    authors: Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

    更新日期:2006-10-01 00:00:00

  • Cold exposure as a potentiating factor of pineal actions in nonhibernating mammals.

    abstract::Twenty-eight-day-old male rats were used in three experiments to study whether cold exposure potentiates pineal actions in nonhibernating mammals. The following questions were considered: (a) Can cold exposure increase the antigonadal effects of light deprivation? (b) Are the effects induced by blindness plus cold exp...


    pub_type: 杂志文章


    authors: Sánchez-Barceló EJ,Cos S,Mediavilla MD

    更新日期:1986-02-01 00:00:00

  • Cryopreservation of chum salmon blastomeres by the straw method.

    abstract::In order to preserve genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated. Survival rates under various conditions were compared: the nature and the concentration of cryoprotectants before and after freezing, the seeding temperature, and...


    pub_type: 杂志文章


    authors: Kusuda S,Teranishi T,Koide N

    更新日期:2002-08-01 00:00:00

  • Cryopreservation of economically valuable marine micro-algae in the classes Bacillariophyceae, Chlorophyceae, Cyanophyceae, Dinophyceae, Haptophyceae, Prasinophyceae, and Rhodophyceae.

    abstract::The ability to routinely cryopreserve micro-algal species reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift. Cryopreservation is also a useful adjunct in aquaculture hatcheries for strains of micro-a...


    pub_type: 杂志文章


    authors: Rhodes L,Smith J,Tervit R,Roberts R,Adamson J,Adams S,Decker M

    更新日期:2006-02-01 00:00:00

  • Percutaneous ablative cryoimmunotherapy for micrometastaic abscopal effect: No complications.

    abstract::Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...


    pub_type: 杂志文章


    authors: Soule E,Bandyk M,Matteo J

    更新日期:2018-06-01 00:00:00

  • The effects of TETs on DNA methylation and hydroxymethylation of mouse oocytes after vitrification and warming.

    abstract::Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...


    pub_type: 杂志文章


    authors: Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

    更新日期:2019-10-01 00:00:00

  • Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cells.

    abstract::We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...


    pub_type: 杂志文章


    authors: Katkov II,Kim MS,Bajpai R,Altman YS,Mercola M,Loring JF,Terskikh AV,Snyder EY,Levine F

    更新日期:2006-10-01 00:00:00

  • Clinical utility of freeze-all approach in ART treatment: A mini-review.

    abstract::A significant proportion of couples at reproductive age rely on assisted reproductive technology to overcome infertility. In vitro fertilisation (IVF) involves typically the use of exogenous gonadotropins to stimulate the ovary to produce oocytes, which are collected surgically. After fertilization by conventional IVF...


    pub_type: 杂志文章,评审


    authors: Sciorio R,Esteves SC

    更新日期:2020-02-01 00:00:00

  • Comprehensive treatment of malignant mesothelioma patients after the failure of systemic chemotherapy.

    abstract::Malignant mesothelioma (MM) is an aggressive neoplasm usually arising from the mesothelial surfaces of the pleural or peritoneal cavity. Currently, no standard therapy is available. The most commonly used therapy is cytoreductive surgery combined with systematic chemotherapy, but the median overall survival (OS) is le...


    pub_type: 杂志文章


    authors: Chen J,Liang B,Yuan Y,Liu C,Li L,Li H,Mu F,Zuo J,Xu K

    更新日期:2012-12-01 00:00:00

  • Percutaneous comprehensive cryoablation for metastatic esophageal cancer after failure of radical surgery.

    abstract::Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...


    pub_type: 杂志文章


    authors: Jiongyuan X,Lizhi N,Feng M,Shupeng L,Yin L,Mengtian L,Jianying Z,Fei Y,Jibing C,Jialiang L,Kecheng X

    更新日期:2013-12-01 00:00:00

  • Active control of the nucleation temperature enhances freezing survival of multipotent mesenchymal stromal cells.

    abstract::Cryopreservation is a technique that has been extensively used for storage of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine. Therefore, improving current cryopreservation procedures in terms of increasing cell viability and functionality is important. In this study, we optimized the cryopreserv...


    pub_type: 杂志文章


    authors: Lauterboeck L,Hofmann N,Mueller T,Glasmacher B

    更新日期:2015-12-01 00:00:00

  • Peripheral tissue freezing in cryosurgery.

    abstract::The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...


    pub_type: 杂志文章


    authors: Song WJ,Jiji LM

    更新日期:1988-04-01 00:00:00

  • Effect of the desiccation tolerance and cryopreservation methods on the viability of Citrus limon L. Burm cv. Eureka seeds.

    abstract::Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influenc...


    pub_type: 杂志文章


    authors: Orjuela-Palacio JM,Graiver N,Santos MV,Zaritzky NE

    更新日期:2019-08-01 00:00:00

  • Cryopreservation of human adipose tissues.

    abstract::Scientific studies on cryopreservation of adipose tissues have seldom been performed. The purpose of our present study is conducted both in vitro and in vivo to develop a novel cryopreservation method that can be used successfully for long-term preservation of human adipose tissues for possible future clinical applica...


    pub_type: 杂志文章


    authors: Cui XD,Gao DY,Fink BF,Vasconez HC,Pu LL

    更新日期:2007-12-01 00:00:00

  • Effect of cooling rates on the cold hardiness and cryoprotectant profiles of locust eggs.

    abstract::To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...


    pub_type: 杂志文章


    authors: Wang HS,Kang L

    更新日期:2005-10-01 00:00:00