当前位置： SCI文献检索 > CRYOBIOLOGY期刊下所有文献 > Metabolic differences between cold stored and machine perfused porcine kidneys: A 1H NMR based study.

Metabolic differences between cold stored and machine perfused porcine kidneys: A 1H NMR based study.

Abstract：

:Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two hours following retrieval. Paired kidneys then underwent 24 h of HMP or SCS or served as time zero controls. Metabolite quantification in both storage fluid and kidney tissue was performed using one dimensional 1H NMR spectroscopy. For each metabolite, the net gain for each storage modality was determined by comparing the total amount in each closed system (i.e. total amount in storage fluid and kidney combined) compared with controls. 26 metabolites were included for analysis. Total system metabolite quantities following HMP or SCS were greater for 14 compared with controls (all p < 0.05). In addition to metabolic differences with control kidneys, the net metabolic gain during HMP was greater than SCS for 8 metabolites (all p < 0.05). These included metabolites related to central metabolism (lactate, glutamate, aspartate, fumarate and acetate). The metabolic environments of both perfusion fluid and the kidney tissue are strikingly different between SCS and HMP systems in this animal model. The total amount of central metabolites such as lactate and glutamate observed in the HMP kidney system suggests a greater degree of de novo metabolic activity than in the SCS system. Maintenance of central metabolic pathways may contribute to the clinical benefits of HMP.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Nath J,Smith TB,Patel K,Ebbs SR,Hollis A,Tennant DA,Ludwig C,Ready AR

doi

10.1016/j.cryobiol.2016.11.006

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

115-120

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(16)30439-4

journal_volume

74

pub_type

杂志文章

相关文献

CRYOBIOLOGY文献大全
  • A study on the vitrification of stage III zebrafish (Danio rerio) ovarian follicles.

    abstract：:Attempts to cryopreserve fish embryos have been conducted over the past three decades, nevertheless successful cryopreservation protocol for long-term storage still remains elusive. Fish oocytes offer some advantages when compared to embryos, which may help in improving the chances of cryopreservation. In the present ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.10.002

    authors： Godoy LC,Streit DP Jr,Zampolla T,Bos-Mikich A,Zhang T

    更新日期：2013-12-01 00:00:00

  • Percutaneous ablative cryoimmunotherapy for micrometastaic abscopal effect: No complications.

    abstract：:Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.04.013

    authors： Soule E,Bandyk M,Matteo J

    更新日期：2018-06-01 00:00:00

  • Osmotic injury of PC-3 cells by hypertonic NaCl solutions at temperatures above 0 degrees C.

    abstract：:Cell injury due to osmotic dehydration, which is regarded as a major cause of injury during freeze-thaw processes, was examined closely using a perfusion microscope. Human prostatic adenocarcinoma cells (PC-3), which were put in a chamber, were subjected to hyperosmotic stresses by perfusing NaCl solutions of varying ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2004.10.004

    authors： Zawlodzka S,Takamatsu H

    更新日期：2005-02-01 00:00:00

  • Trehalose loading into red blood cells is accompanied with hemoglobin oxidation and membrane lipid peroxidation.

    abstract：:One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study w...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.12.003

    authors： Kanias T,Acker JP

    更新日期：2009-04-01 00:00:00

  • Functional preservation of the mammalian kidney. IV. Functional effects of perfusion with dimethyl sulfoxide (DMSO) at normothermia.

    abstract：:Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(76)90101-2

    authors： Karow AM Jr,Jeske AH

    更新日期：1976-08-01 00:00:00

  • Comparative analysis of the transcriptome of the overwintering desert beetle Microdera punctipennis.

    abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2017.06.009

    authors： Tusong K,Guo X,Meng S,Liu X,Ma J

    更新日期：2017-10-01 00:00:00

  • Effectiveness of a tris-based extender (SHOTOR diluent) for the preservation of Bactrian camel (Camelus bactrianus) semen.

    abstract：:The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2006.03.006

    authors： Niasari-Naslaji A,Mosaferi S,Bahmani N,Gharahdaghi AA,Abarghani A,Ghanbari A,Gerami A

    更新日期：2006-08-01 00:00:00

  • Vitrification of mature mouse oocytes in a 6 M Me2SO solution supplemented with antifreeze glycoproteins: the effect of temperature.

    abstract：:Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) (...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2098

    authors： O'Neil L,Paynter SJ,Fuller BJ,Shaw RW,DeVries AL

    更新日期：1998-08-01 00:00:00

  • Ice restructuring inhibition activities in antifreeze proteins with distinct differences in thermal hysteresis.

    abstract：:Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2010.10.158

    authors： Yu SO,Brown A,Middleton AJ,Tomczak MM,Walker VK,Davies PL

    更新日期：2010-12-01 00:00:00

  • Study of cryopreservation of articular chondrocytes using the Taguchi method.

    abstract：:This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes using the Taguchi method. Freeze-thaw experiments based on the L(8)(2(7)) two-level orthogonal array of the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statisticall...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.10.008

    authors： Lyu SR,Wu WT,Hou CC,Hsieh WH

    更新日期：2010-04-01 00:00:00

  • Cryopreservation of calf testicular tissues with knockout serum replacement.

    abstract：:To enrich bovine gonocytes from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined. Using Minimum essential medium (MEM) + 10% dimethyl sulfoxide (Me2SO) as the basic medium, calf testicular tissues were cryopreserved in media co...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.01.010

    authors： Jiang Y,Zhu WQ,Zhu XC,Cai NN,Yang R,Cai H,Zhang XM

    更新日期：2020-02-01 00:00:00

  • Insights into the model of non-perfect osmometer cells for cryopreservation: A parametric sweep analysis.

    abstract：:Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechano...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.11.013

    authors： Traversari G,Cincotti A

    更新日期：2020-11-27 00:00:00

  • Non-toxic freezing media to retain the stem cell reserves in adipose tissues.

    abstract：:Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.07.005

    authors： Shaik S,Wu X,Gimble JM,Devireddy R

    更新日期：2020-10-01 00:00:00

  • A re-evaluation of the role of type IV antifreeze protein.

    abstract：:A lipoprotein-like antifreeze protein (type IV AFP) has previously been isolated only from the blood plasma of the longhorn sculpin. However, the plasma antifreeze activity in all individuals of this species tested from Newfoundland and New Brunswick waters ranges from low to undetectable. A close relative of the long...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.10.122

    authors： Gauthier SY,Scotter AJ,Lin FH,Baardsnes J,Fletcher GL,Davies PL

    更新日期：2008-12-01 00:00:00

  • Machine perfusion enhances hepatocyte isolation yields from ischemic livers.

    abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2015.07.006

    authors： Izamis ML,Perk S,Calhoun C,Uygun K,Yarmush ML,Berthiaume F

    更新日期：2015-10-01 00:00:00

  • In vitro colony formation by cryopreserved primary human tumor cells.

    abstract：:The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of n...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(84)90215-3

    authors： Allen ED,Gau TC,Natale RB

    更新日期：1984-04-01 00:00:00

  • Piglets produced by transfer of vitrified porcine embryos after stepwise dilution of cryoprotectants.

    abstract：:A total of 498 porcine embryos at various stages of development collected from superovulated gilts was used to investigate cryopreservation. First, blastocysts (BL), expanded blastocysts (ExB), and hatched blastocysts (HB) were used to determine the effect of exposure to concentrated solutions of ethylene glycol as cr...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1997.2056

    authors： Kobayashi S,Takei M,Kano M,Tomita M,Leibo SP

    更新日期：1998-02-01 00:00:00

  • Dependence of trehalose protective action on the initial phase state of dipalmitoylphosphatidylcholine bilayers.

    abstract：:Dipalmitoylphosphatidylcholine (DPPC) bilayers hydrated in the presence of trehalose were equilibrated at various temperatures (4, 20, and 60 degrees C) corresponding to the crystalline Lc, gel L beta', and liquid-crystalline L alpha phases, respectively, and then desiccated at these temperatures or freeze-dried at -8...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(88)90033-8

    authors： Tsvetkova N,Tenchov B,Tsonev L,Tsvetkov T

    更新日期：1988-06-01 00:00:00

  • Trehalose transporter from African chironomid larvae improves desiccation tolerance of Chinese hamster ovary cells.

    abstract：:Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disacchari...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2011.11.007

    authors： Chakraborty N,Menze MA,Elmoazzen H,Vu H,Yarmush ML,Hand SC,Toner M

    更新日期：2012-04-01 00:00:00

  • Isolation of ice-nucleating active bacteria from the freeze-tolerant frog, Rana sylvatica.

    abstract：:Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1995.1036

    authors： Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

    更新日期：1995-08-01 00:00:00

  • Effects of centrifugation and lipid removal on the cryopreservation of in vitro produced bovine embryos at the eight-cell stage.

    abstract：:The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes were centrifuged at 16,000 g for 20 min for the cytoplasmic lipid polarization and their lipid lay...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2077

    authors： Murakami M,Otoi T,Sumantri C,Suzuki T

    更新日期：1998-05-01 00:00:00

  • Vitrification properties of solutions of ethylene glycol in saline containing PVP, Ficoll, or dextran.

    abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1997.2043

    authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

    更新日期：1997-11-01 00:00:00

  • Glass-forming tendency and stability of the amorphous state in the aqueous solutions of linear polyalcohols with four carbons. I. Binary systems water-polyalcohol.

    abstract：:All the aqueous solutions of linear saturated polyalcohols with four carbons have been investigated at low temperature. Only ice has been observed in the solutions of 1,3-butanediol and 1,2,3- and 1,2,4-butanetriol. For same solute concentration, the glass-forming tendency on cooling is highest with 2,3-butanediol, wh...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(86)90031-3

    authors： Boutron P,Mehl P,Kaufmann A,Angibaud P

    更新日期：1986-10-01 00:00:00

  • Cryopreservation of turkey semen: effect of breeding line and freezing method on post-thaw sperm quality, fertilization, and hatching.

    abstract：:Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw function of sperm from fi...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.04.003

    authors： Long JA,Purdy PH,Zuidberg K,Hiemstra SJ,Velleman SG,Woelders H

    更新日期：2014-06-01 00:00:00

  • Effect of cooling rates on the cold hardiness and cryoprotectant profiles of locust eggs.

    abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2005.07.003

    authors： Wang HS,Kang L

    更新日期：2005-10-01 00:00:00

  • Some insight into the physical basis of the cryoprotective action of dimethyl sulfoxide and ethylene glycol.

    abstract：:In the determination of the solid-liquid phase equilibria in the aqueous mixtures of dimethyl sulfoxide (Me2SO) and ethylene glycol (EG) one often encounters the problem of equilibrium crystallization. In the present report the above aqueous solutions are equilibrated for crystallization in a dielectric cell during wh...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1997.2064

    authors： Murthy SS

    更新日期：1998-03-01 00:00:00

  • Comparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezing.

    abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.09.001

    authors： Yildiz C,Mullen B,Jarvi K,McKerlie C,Lo KC

    更新日期：2018-10-01 00:00:00

  • Development of vitrified mouse oocytes after in vitro fertilization.

    abstract：:Mouse oocytes were cryopreserved by the vitrification method using vitrification solution (VSI) and the effects of dilution methods were examined on the rate of in vitro and in vivo development. Eighty-three percent and 75% of vitrified oocytes exhibited normal morphology when diluted in glycerol + sucrose and sucrose...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(91)90007-b

    authors： Kono T,Kwon OY,Nakahara T

    更新日期：1991-02-01 00:00:00

  • Water permeability, Lp, of the mouse sperm plasma membrane and its activation energy are strongly dependent on interaction of the plasma membrane with the sperm cytoskeleton.

    abstract：:Two parameters fundamental to cell cryobiology are the water permeability (hydraulic conductivity), Lp, and its activation energy, EA. The Lp can be calculated from two experimental determinations: the critical osmolality, Osmcrit, at which 50% of the cells lyse, and the time, tcrit, to 50% lysis in a highly hyposmoti...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1997.2033

    authors： Noiles EE,Thompson KA,Storey BT

    更新日期：1997-08-01 00:00:00

  • A 9 kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni.

    abstract：:A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.07.001

    authors： Hawes TC,Marshall CJ,Wharton DA

    更新日期：2014-08-01 00:00:00