A 9 kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni.

Abstract:

:A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techniques. Mass spectrometry identified a single 9 kDa component in the purified ice fraction. Intramolecular disulphide bonding was suggested by the presence of 12 cysteine residues. The specific amino acid composition is unique, particularly with regard to the presence of histidine (11.5%). But it also shows noticeable commonalities with insect AFPs in the abundance of cysteine (13.8%), while simultaneously hinting, through the presence of glycine (11.5%), that the metabolic building blocks of AFPs in Collembola may have a phylogenetically-determined component.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Hawes TC,Marshall CJ,Wharton DA

doi

10.1016/j.cryobiol.2014.07.001

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

181-3

issue

1

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(14)00131-X

journal_volume

69

pub_type

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