Abstract：
:The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of nonfrozen controls. The methods used in this study do not require elaborate freezing equipment, and can be used for the cryopreservation of a wide variety of types of cancers.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Allen ED,Gau TC,Natale RBdoi
10.1016/0011-2240(84)90215-3subject
Has Abstractpub_date
1984-04-01 00:00:00pages
240-5issue
2eissn
0011-2240issn
1090-2392pii
0011-2240(84)90215-3journal_volume
21pub_type
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