Abstract：
:To enrich bovine gonocytes from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined. Using Minimum essential medium (MEM) + 10% dimethyl sulfoxide (Me2SO) as the basic medium, calf testicular tissues were cryopreserved in media containing 0, 5, 10, 20, 40, 90% KSR and 5% fetal bovine serum (FBS) respectively. Morphologically, the seminiferous cords and interstitium were well preserved in all groups. The gonocytes were all glial cell line-derived neurotrophic factor (GDNF) family receptor α-1 (GFRα-1) positive. The recovery rates in all KSR groups were higher than that of the 10% Me2SO group, while comparable to the 5% FBS group. The enriched gonocytes expressed gonocyte marker GFRα-1 typically. Collectively, supplementation of 5-10% KSR can achieve comparable cryoprotective effects with using 5% FBS, which is useful in future study due to its defined formulation that is more consistent in quality and stable in supply.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Jiang Y,Zhu WQ,Zhu XC,Cai NN,Yang R,Cai H,Zhang XMdoi
10.1016/j.cryobiol.2020.01.010subject
Has Abstractpub_date
2020-02-01 00:00:00pages
255-257eissn
0011-2240issn
1090-2392pii
S0011-2240(19)30506-1journal_volume
92pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.06.006
更新日期：2005-10-01 00:00:00
abstract：:Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.10.017
更新日期：2020-11-04 00:00:00
abstract：:Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.07.005
更新日期：2020-10-01 00:00:00
abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1016/j.cryobiol.2020.04.009
更新日期：2020-06-01 00:00:00
abstract：:Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.01.002
更新日期：2017-04-01 00:00:00
abstract：:The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.2000.2247
更新日期：2000-06-01 00:00:00
abstract：:Despite some 26 published reports addressing oyster sperm cryopreservation, systematic factor optimization is lacking, and sperm cryopreservation has not yet found application in aquaculture on a commercial scale. In this study, the effects of cooling rate, single or combined cryoprotectants at various concentrations,...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.06.007
更新日期：2005-10-01 00:00:00
abstract：:One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.05.007
更新日期：2008-08-01 00:00:00
abstract：:Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (-196 °C). The organic solvent dimethyl sulfoxide (Me2SO) is o...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.01.001
更新日期：2017-04-01 00:00:00
abstract：:Previous reports established that butylated hydroxy toluene (BHT) minimized cold-induced membrane rupture in sperm from several species. No data regarding the specificity of its effect is available. In this study 25 BHT analogs were tested for their effect on bovine sperm membrane stability. Fourteen were membrane lyt...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(92)90010-y
更新日期：1992-02-01 00:00:00
abstract：:Cryopreservation is a technique that has been extensively used for storage of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine. Therefore, improving current cryopreservation procedures in terms of increasing cell viability and functionality is important. In this study, we optimized the cryopreserv...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.10.145
更新日期：2015-12-01 00:00:00
abstract：:Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.06.003
更新日期：2013-10-01 00:00:00
abstract：:Devitrification (ice formation during warming) is one of the primary obstacles to successful organ vitrification (solidification without ice formation). The only feasible approach to overcoming either devitrification or its damaging effects in a large organ appears at present to be the use of some form of electromagne...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(90)90035-3
更新日期：1990-10-01 00:00:00
abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.07.003
更新日期：2005-10-01 00:00:00
abstract：:Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.05.001
更新日期：2018-05-06 00:00:00
abstract：:Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2003.12.001
更新日期：2004-02-01 00:00:00
abstract：:Percutaneous ablation is the currently preferred locoregional therapy for non-resectable hepatocellular cancer (HCC). Cryoablation is an attractive option because it forms an ice ball viewable by many imaging methods. This study assessed the therapeutic effect of comprehensive cryoablation (of intra- and extrahepatic ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2012.11.008
更新日期：2013-02-01 00:00:00
abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2163
更新日期：1999-06-01 00:00:00
abstract：:We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2182
更新日期：1999-08-01 00:00:00
abstract：:Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol h...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.05.006
更新日期：2013-08-01 00:00:00
abstract：BACKGROUND:The effect of dimethyl sulfoxide (Me2SO) on enumeration of post-thaw CD45+ and CD34+ cells of umbilical cord blood (HPC-C) and mobilized peripheral blood (HPC-A) has not been systematically studied. METHODS:Cells from leukapheresis products from multiple myeloma patients and umbilical cord blood cells were ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.06.003
更新日期：2005-10-01 00:00:00
abstract：:Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/s0011-2240(02)00017-2
更新日期：2002-04-01 00:00:00
abstract：:Intracerebral grafting of immature brain tissue is now widely used as a tool to study neuronal development and regeneration in the brain and spinal cord. This has stimulated the interest in methods for storage of such tissue before transplantation. In this study a method for cryopreservation of immature rat central ne...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(87)90014-9
更新日期：1987-04-01 00:00:00
abstract：:This study examines the effectiveness of the cryotop vitrification method for the cryopreservation of goat blastocysts. To determine the effects of embryo development stage and donor age on in vitro survival rates, good-quality blastocysts from adult and prepubertal goats were sorted into non-expanded, expanded, hatch...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2011.09.002
更新日期：2011-12-01 00:00:00
abstract：:Hepatic cryotherapy is increasingly used in the treatment of patients with multiple hepatic metastases, particularly from colorectal cancer. The Cryotech LCS 2000 system, with insulated shaft-circulated liquid nitrogen probes, was designed for this purpose and was evaluated on the bench and in an animal model. The 9-m...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1993.1007
更新日期：1993-02-01 00:00:00
abstract：:One thousand cryohemorrhoidectomies were performed in the past 7 years (1973-1979). Ninety percent of the patients were satisfied with the technique; approximately one-third of the patients experienced severe to moderate pain and 11% had recurrences or related problems. The majority of patients with postoperative pain...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(82)90154-7
更新日期：1982-06-01 00:00:00
abstract：:Sustainability of channel catfish, Ictalurus punctatus ♀ × blue catfish, Ictalurus furcatus ♂ hybrid aquaculture relies on new innovative technologies to maximize fry output. Transplanting spermatogonial stem cells (SSCs) from blue catfish into channel catfish hosts has the potential to greatly increase gamete availab...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.10.010
更新日期：2020-12-01 00:00:00
abstract：:Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, punctu...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(83)90031-7
更新日期：1983-08-01 00:00:00
abstract：:Cryopreservation has been reported to damage approximately 40-50% of viable sperm in bull semen. The present study was undertaken to assess the cryo-effectiveness of glycerol (GLY), ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propylene glycol (PND) as cryoprotectant during the cryopreservation of Nguni bull se...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2016.04.001
更新日期：2016-06-01 00:00:00
abstract：:The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2011.12.001
更新日期：2012-04-01 00:00:00
