Thermal study of simple amino-alcohol solutions.

abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

journal_title：Cryobiology

authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

更新日期：2007-10-01 00:00:00

abstract：:Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilizati...

journal_title：Cryobiology

authors： Moawad AR,Fisher P,Zhu J,Choi I,Polgar Z,Dinnyes A,Campbell KH

更新日期：2012-10-01 00:00:00

abstract：:Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two ho...

journal_title：Cryobiology

authors： Nath J,Smith TB,Patel K,Ebbs SR,Hollis A,Tennant DA,Ludwig C,Ready AR

更新日期：2017-02-01 00:00:00

abstract：:Renal epithelial cells from donor kidneys are susceptible to hypothermic preservation injury, which is attenuated when they over express the cytoskeletal linker protein ezrin. This study was designed to characterize the mechanisms of this protection. Renal epithelial cell lines were created from LLC-PK1 cells, which e...

journal_title：Cryobiology

authors： Tian T,Lindell SL,Lam M,Mangino MJ

更新日期：2012-08-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:Devitrification (ice formation during warming) is one of the primary obstacles to successful organ vitrification (solidification without ice formation). The only feasible approach to overcoming either devitrification or its damaging effects in a large organ appears at present to be the use of some form of electromagne...

journal_title：Cryobiology

authors： Ruggera PS,Fahy GM

更新日期：1990-10-01 00:00:00

abstract：:Twenty-eight-day-old male rats were used in three experiments to study whether cold exposure potentiates pineal actions in nonhibernating mammals. The following questions were considered: (a) Can cold exposure increase the antigonadal effects of light deprivation? (b) Are the effects induced by blindness plus cold exp...

journal_title：Cryobiology

authors： Sánchez-Barceló EJ,Cos S,Mediavilla MD

更新日期：1986-02-01 00:00:00

abstract：:Wood frogs (Rana sylvatica) can endure weeks of subzero temperature exposure during the winter with up to 65% of their body water frozen as extracellular ice. Associated with freezing survival is elevated expression of a number of genes/proteins including the unidentified gene, li16, first described in liver. The curr...

journal_title：Cryobiology

authors： Sullivan KJ,Storey KB

更新日期：2012-06-01 00:00:00

abstract：:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...

journal_title：Cryobiology

authors： Storey KB

更新日期：2004-04-01 00:00:00

abstract：:Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influenc...

journal_title：Cryobiology

authors： Orjuela-Palacio JM,Graiver N,Santos MV,Zaritzky NE

更新日期：2019-08-01 00:00:00

abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...

journal_title：Cryobiology

authors： Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

更新日期：2020-12-04 00:00:00

abstract：:The utility of microalgal biomass and bioproducts depends on long-term maintenance of certain physiological or biochemical features of the species. While unique characteristics may not be durably maintained with general subculture, cryopreservation methods better prevent alterations from desired characteristics. Post-...

journal_title：Cryobiology

authors： Zheng L,Lu Z,Zhang Q,Li T,Song L

更新日期：2018-04-01 00:00:00

abstract：:Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disacchari...

journal_title：Cryobiology

authors： Chakraborty N,Menze MA,Elmoazzen H,Vu H,Yarmush ML,Hand SC,Toner M

更新日期：2012-04-01 00:00:00

abstract：:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facil...

journal_title：Cryobiology

authors： Caruana M,Battle T,Fuller B,Davidson B

更新日期：1999-03-01 00:00:00

abstract：:In the determination of the solid-liquid phase equilibria in the aqueous mixtures of dimethyl sulfoxide (Me2SO) and ethylene glycol (EG) one often encounters the problem of equilibrium crystallization. In the present report the above aqueous solutions are equilibrated for crystallization in a dielectric cell during wh...

journal_title：Cryobiology

authors： Murthy SS

更新日期：1998-03-01 00:00:00

abstract：BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...

journal_title：Cryobiology

authors： Solanes N,Rigol M,Khabiri E,Castellà M,Ramírez J,Roqué M,Agustí E,Roig E,Pérez-Villa F,Segalés J,Pomar JL,Engel P,Massaguer A,Martorell J,Rodríguez JA,Sanz G,Heras M

更新日期：2005-10-01 00:00:00

abstract：:The Arrhenius plots of inactivation (killing) rates of five mammalian cell lines, V-79 Chinese hamster lung, mouse L-929, mouse neuroblastoma, human 18LU, and human erythrocytes, exposed to hypothermia contain a break somewhere between 5 and 10 degrees C caused by a change from positive to negative slope, which corres...

journal_title：Cryobiology

authors： Kruuv J,Glofcheski DJ,Lepock JR

更新日期：1995-04-01 00:00:00

abstract：:The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...

journal_title：Cryobiology

authors： Worland MR,Block W

更新日期：1999-02-01 00:00:00

abstract：:Trypsin activity oscillations are shown by the autocatalytic activation of trypsinogen at 0 degrees C in aqueous solution. The oscillations were observed for 3-4 days and show only slight decrease in enzyme activity. The zymogen has been kept at ice water temperature and pH 8.2 in the presence of Mn2+ ion. The mean pe...

journal_title：Cryobiology

authors： Vajda T

更新日期：1986-06-01 00:00:00

abstract：:(1) Tyrosine and tryptophan metabolism in brain and peripheral tissues were studied in hypothermic hibernating and normothermic nonhibernating 13-lined ground squirrels (Spermophilus tridecemlineatus). (2) In the hypothermic hibernating state, there were significant elevations of brain stem tyrosine, norepinephrine, a...

journal_title：Cryobiology

authors： Weekley BL,Harlow HJ

更新日期：1987-12-01 00:00:00

abstract：:Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...

journal_title：Cryobiology

authors： Woods EJ,Liu J,Gilmore JA,Reid TJ,Gao DY,Critser JK

更新日期：1999-05-01 00:00:00

abstract：:The objective of this study was to determine osmotic tolerance of canine spermatozoa. The study comprised three experiments: (1) spermatozoa suspended either in an egg yolk-citrate (EYC) extender or in Kenney skim milk extender were exposed to NaCl solutions ranging from 290 to 1500 mOsm; (2) spermatozoa suspended in ...

journal_title：Cryobiology

authors： Songsasen N,Yu I,Murton S,Paccamonti DL,Eilts BE,Godke RA,Leibo SP

更新日期：2002-02-01 00:00:00

abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

journal_title：Cryobiology

authors： Wusteman M,Boylan S,Pegg DE

更新日期：1996-08-01 00:00:00

abstract：:We make some crucial remarks about the recent review by Buitink and Leprince (2004) [Cryobiology 48 (2004) 215-228] considering their false definition about the glass transition temperature based on the value of (shear) viscosity: 1014 Pa⋅s. The correct value is 1012 Pa⋅s as evidenced from http://goldbook.iupac.org/ht...

journal_title：Cryobiology

authors： Kwang-Hua CW

更新日期：2018-08-01 00:00:00

abstract：:Boar spermatozoa are extremely sensitive to low temperatures and the cryopreservation causes dramatic changes in sperm survivability, but it is not clear which part of the cryopreservation process affects the most. The aim of this work was to assess early events of apoptotic changes as damage indicators in boar sperm ...

journal_title：Cryobiology

authors： Malcervelli DM,Torres P,Suhevic JF,Cisale H,Fischman ML

更新日期：2020-08-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...

journal_title：Cryobiology

authors： Pomorski L,Bartos M,Matejkowska M,Amsolik M,Kuzdak K

更新日期：1999-11-01 00:00:00

abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...

journal_title：Cryobiology

authors： Hunter JE,Bernard A,Fuller BJ,McGrath JJ,Shaw RW

更新日期：1992-04-01 00:00:00

abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...

journal_title：Cryobiology

authors： Robles V,Cabrita E,Real M,Alvarez R,Herráez MP

更新日期：2003-08-01 00:00:00