Platelet cryopreservation with glycerol, dextran, and mannitol: recovery of 5-hydroxytryptamine uptake and hypotonic stress response.

abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

journal_title：Cryobiology

authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

更新日期：2020-11-26 00:00:00

abstract：:The purpose of our study was to investigate underlying basic mechanisms of hypothermia-induced cardioprotection during oxidative stress in a cardiomyocyte cell culture model. For hypothermic treatment we cooled H9c2 cardiomyocytes to 20°C, maintained 20min at 20°C during which short-term oxidative damage was inflicted...

journal_title：Cryobiology

authors： Diestel A,Drescher C,Miera O,Berger F,Schmitt KR

更新日期：2011-02-01 00:00:00

abstract：:The efficacy of attenuated strain of gdhA derivative Pasteurella multocida B:2 mutant as a live vaccine to control haemorrhagic septicaemia (HS) disease in cattle and buffaloes has been demonstrated. In order to use P. multocida B:2 mutant as a commercial product, it is essential to optimise its formulation for high v...

journal_title：Cryobiology

authors： Oslan SNH,Halim M,Ramle NA,Saad MZ,Tan JS,Kapri MR,Ariff AB

更新日期：2017-12-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:In order to evaluate the many variables that can affect cryopreservation success, a simple, highly reproducible model system is required. We have evaluated the use of tetrazolium reductase activity as a prognostic indicator of skin viability in an inbred murine model. Two inbred hairless mouse strains were characteriz...

journal_title：Cryobiology

authors： Kearney JN,Wheldon LA,Gowland G

更新日期：1990-02-01 00:00:00

abstract：:Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...

journal_title：Cryobiology

authors： Purdy PH,Graham JK

更新日期：2004-02-01 00:00:00

abstract：:Mouse oocytes were cryopreserved by the vitrification method using vitrification solution (VSI) and the effects of dilution methods were examined on the rate of in vitro and in vivo development. Eighty-three percent and 75% of vitrified oocytes exhibited normal morphology when diluted in glycerol + sucrose and sucrose...

journal_title：Cryobiology

authors： Kono T,Kwon OY,Nakahara T

更新日期：1991-02-01 00:00:00

abstract：:The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice-nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleatin...

journal_title：Cryobiology

authors： Weng L,Tessier SN,Swei A,Stott SL,Toner M

更新日期：2017-04-01 00:00:00

abstract：:A 2,3-butanediol containing 96.7% (w/w) racemic mixture of the levo and dextro isomers and only 3.1% (w/w) of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 degrees C at rates between 2 and 3500 degrees C/min, followed by slo...

journal_title：Cryobiology

authors： Boutron P

更新日期：1992-06-01 00:00:00

abstract：:Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...

journal_title：Cryobiology

authors： Chan JY,Ooi EH

更新日期：2016-12-01 00:00:00

abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

journal_title：Cryobiology

authors： Mazur P,Schneider U,Mahowald AP

更新日期：1992-02-01 00:00:00

abstract：:The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...

journal_title：Cryobiology

authors： Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

更新日期：2001-11-01 00:00:00

abstract：:The general belief is that slow freezing injury is either the result of exposure to high salt concentrations or the result of excessive cell shrinkage. Increased salt concentration arises as increasing amounts of pure ice precipitate out of solution during freezing and cause the liquid-filled channels in which the cel...

journal_title：Cryobiology

authors： Mazur P,Rigopoulos N

更新日期：1983-06-01 00:00:00

abstract：:We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...

journal_title：Cryobiology

authors： Odintsova NA,Boroda AV,Maiorova MA,Yakovlev KV

更新日期：2017-08-01 00:00:00

abstract：:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. ...

journal_title：Cryobiology

authors： Zeng C,Peng W,Ding L,He L,Zhang Y,Fang D,Tang K

更新日期：2014-08-01 00:00:00

abstract：:Toxoplasma gondii is a protozoan parasite that infects birds and mammals, including humans. T. gondii T-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure for T. gondii T-263 bradyzoites has been developed to meet the require...

journal_title：Cryobiology

authors： Booth KS,James ER,Popiel I

更新日期：1996-06-01 00:00:00

abstract：:Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the cult...

journal_title：Cryobiology

authors： Del Pino A,Ligero G,López MB,Navarro H,Carrillo JA,Pantoll SC,Díaz de la Guardia R

更新日期：2015-02-01 00:00:00

abstract：:Malignant mesothelioma (MM) is an aggressive neoplasm usually arising from the mesothelial surfaces of the pleural or peritoneal cavity. Currently, no standard therapy is available. The most commonly used therapy is cytoreductive surgery combined with systematic chemotherapy, but the median overall survival (OS) is le...

journal_title：Cryobiology

authors： Chen J,Liang B,Yuan Y,Liu C,Li L,Li H,Mu F,Zuo J,Xu K

更新日期：2012-12-01 00:00:00

abstract：:The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejacu...

journal_title：Cryobiology

authors： Esteso MC,Toledano-Díaz A,Castaño C,Pradiee J,Lopez-Sebastián A,Santiago-Moreno J

更新日期：2018-02-01 00:00:00

abstract：:A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...

journal_title：Cryobiology

authors： Hawes TC,Marshall CJ,Wharton DA

更新日期：2014-08-01 00:00:00

abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...

journal_title：Cryobiology

authors： Huang KY,de Groot SA,Woelders H,van der Horst GT,Themmen AP,Colenbrander B,van Vlissingen JM

更新日期：2010-04-01 00:00:00

abstract：:The [K+]/[Na+] ratio of rabbit renal cortical slices was used to examine, at 25 degrees C, the effects on viability of three cryoprotectant agents (CPA) (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) in combination with three vehicle solutions (Krebs-Henseleit (K-H), solution A, and RPS-2). Viability asse...

journal_title：Cryobiology

authors： Clark P,Fahy GM,Karow AM Jr

更新日期：1984-06-01 00:00:00

abstract：:Previous studies into the mechanisms governing the freezing of cells in the absence of extracellular ice have been extended to develop a method for the preservation of viable cells in the undercooled state. Deep undercooling of cells is achieved by suspending fine droplets of the cells in oil to make an emulsion, thus...

journal_title：Cryobiology

authors： Mathias SF,Franks F,Hatley RH

更新日期：1985-12-01 00:00:00

abstract：:The present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and L...

journal_title：Cryobiology

authors： Kocyigit A,Cevik M

更新日期：2015-08-01 00:00:00

abstract：:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...

journal_title：Cryobiology

authors： Storey KB

更新日期：2004-04-01 00:00:00

abstract：:Human mesenchymal stem cell (hMSC) research has grown exponentially in the last decade. The ability to process and preserve these cells is vital to their use in stem cell therapy. As such, understanding the complex, molecular-based stress responses associated with biopreservation is necessary to improve outcomes and m...

journal_title：Cryobiology

authors： Corwin WL,Baust JM,Baust JG,Van Buskirk RG

更新日期：2014-04-01 00:00:00

abstract：:In an experimental study using porcine corneas we investigated the influence of several variables of freeze-thaw trauma on survival of corneal endothelial cells after corneal cryopreservation employing chondroitin sulfate as a cryoprotectant. We examined the influence of: (1) the concentration of chondroitin sulfate i...

journal_title：Cryobiology

authors： Hagenah M,Böhnke M

更新日期：1993-08-01 00:00:00

abstract：:We have measured the effectiveness of different standard cryogenic liquids (liquid nitrogen, chilled isopentane, chilled hexane, and chilled ethanol) and applied a mathematical model for calculating the temperature changes at different depths in rabbit and rat heads cooled in liquid nitrogen. We have also evaluated th...

journal_title：Cryobiology

authors： Roos MW,Johansson A,Sperber GO

更新日期：1997-09-01 00:00:00

abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

journal_title：Cryobiology

authors： Salmon VM,Leclerc P,Bailey JL

更新日期：2017-02-01 00:00:00

abstract：:Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...

journal_title：Cryobiology

authors： Browne RK,Mahony M,Clulow J

更新日期：2002-06-01 00:00:00