当前位置： SCI文献检索 > CRYOBIOLOGY期刊下所有文献 > Contributions of unfrozen fraction and of salt concentration to the survival of slowly frozen human erythrocytes: influence of warming rate.

Contributions of unfrozen fraction and of salt concentration to the survival of slowly frozen human erythrocytes: influence of warming rate.

Abstract：

:The general belief is that slow freezing injury is either the result of exposure to high salt concentrations or the result of excessive cell shrinkage. Increased salt concentration arises as increasing amounts of pure ice precipitate out of solution during freezing and cause the liquid-filled channels in which the cells are sequestered to dwindle in size. Cell shrinkage is an osmotic response to the concentration of external solutes. The consensus has been that the injury is related to the composition of the solution in these channels and not to the amount of residual liquid. Ordinarily, salt concentration and the amount of liquid in the unfrozen channels are reciprocally related; but they can be separated within limits by varying the total concentration of solutes in the suspending medium while holding the mass ratio of additive to salt constant, and by then slowly freezing samples to various subzero temperatures, chosen to produce various molalities of salt, while holding the unfrozen fraction constant, or vice versa. We have recently reported (9) that when human red cells are frozen under these conditions and thawed rapidly, survival is more dependent on the unfrozen water fraction than it is on the salt concentration in that fraction. The present work compares these results with those obtained with slow thawing. While the general conclusion remains unaltered, slowly thawed cells were able to survive the freezing of a higher fraction of extracellular water than were rapidly thawed cells. Calculations were made of the changes in cell volume during the equilibration with glycerol and the subsequent freezing involved in these experiments. Cell size and cell solute concentration were found to be independent of the fraction of unfrozen extracellular water, but cell survival was strongly dependent on that fraction. If applicable to other than human red cells, this finding is likely to require major modifications in current views of slow-freezing injury and its prevention.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Mazur P,Rigopoulos N

doi

10.1016/0011-2240(83)90016-0

subject

Has Abstract

pub_date

1983-06-01 00:00:00

pages

274-89

issue

3

eissn

0011-2240

issn

1090-2392

pii

0011-2240(83)90016-0

journal_volume

20

pub_type

杂志文章

相关文献

CRYOBIOLOGY文献大全
  • Investigating sperm cryopreservation in a model tunicate, Ciona intestinalis sp. A.

    abstract：:In cryopreservation procedures, the capacity to protect the cells from freezing and thawing processes is sensitive to the choice of the cryoprotective agent (CPA) and to its optimal concentration. The advancement of research on Tunicate model species has raised interest in liquid nitrogen cryopreservation for the stor...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.11.005

    authors： Sorrenti G,Bagnoli A,Miraglia V,Crocetta F,Vitiello V,Ristoratore F,Cirino P,Sansone G,Sordino P

    更新日期：2014-02-01 00:00:00

  • The mechanism by which fish antifreeze proteins cause thermal hysteresis.

    abstract：:Antifreeze proteins are characterised by their ability to prevent ice from growing upon cooling below the bulk melting point. This displacement of the freezing temperature of ice is limited and at a sufficiently low temperature a rapid ice growth takes place. The separation of the melting and freezing temperature is u...

    journal_title：Cryobiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.cryobiol.2005.07.007

    authors： Kristiansen E,Zachariassen KE

    更新日期：2005-12-01 00:00:00

  • Sperm cryopreservation in guppies and black mollies--a generalized freezing protocol for livebearers in Poeciliidae.

    abstract：:In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.09.011

    authors： Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

    更新日期：2009-12-01 00:00:00

  • Effect of cooling rates on the cold hardiness and cryoprotectant profiles of locust eggs.

    abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2005.07.003

    authors： Wang HS,Kang L

    更新日期：2005-10-01 00:00:00

  • Biotransformation activity in vitrified human liver slices.

    abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(91)90026-k

    authors： Wishnies SM,Parrish AR,Sipes IG,Gandolfi AJ,Putnam CW,Krumdieck CL,Brendel K

    更新日期：1991-06-01 00:00:00

  • Cryopreservation of specific pathogen-free (SPF) pig islet cells: effect of culture time before cryopreservation and after thawing.

    abstract：UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1999.2181

    authors： Duvivier V,Darquy S,Reach G

    更新日期：1999-06-01 00:00:00

  • Hypothermia produces rat liver proteomic changes as in hibernating mammals but decreases endoplasmic reticulum chaperones.

    abstract：:Hypothermia is used in the clinic for protection of organs such as the brain against ischemic injury during aortic/complex congenital cardiac surgery or post-resuscitation encephalopathy. The principal mechanism of hypothermic protection is suppression of metabolism, however, the pleiotropic effects of cooling are inc...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2012.05.004

    authors： Oda T,Shimizu K,Yamaguchi A,Satoh K,Matsumoto K

    更新日期：2012-10-01 00:00:00

  • Long-term preservation of dried phosphofructokinase by sugars and sugar/zinc mixtures.

    abstract：:We have demonstrated that sugars and suger/zinc mixtures can be used to preserve the activity of dried phosphofructokinase (PFK) during long-term storage over CaSO4. After 9 weeks in the presence of either 200 mM sucrose or 200 mM trehalose little loss of PFK activity was noted, with almost 60% of the original prefree...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(88)90045-4

    authors： Carpenter JF,Martin B,Loomis SH,Crowe JH

    更新日期：1988-08-01 00:00:00

  • Cryopreservation of Rainbow Trout (Oncorhynchus mykiss) Blastomeres: Influence of Embryo Stage on Postthaw Survival Rate.

    abstract：:The cryopreservation of isolated fish blastomeres is likely to provide a valid alternative to embryo cryopreservation, the results of which are still discouraging. A repeatable technique for the cryopreservation of rainbow trout blastomeres has been established and the effect of embryonic developmental stage on freezi...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2084

    authors： Calvi SL,Maisse G

    更新日期：1998-06-01 00:00:00

  • Visualization of intracellular ice formation using high-speed video cryomicroscopy.

    abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.11.003

    authors： Stott SL,Karlsson JOM

    更新日期：2009-02-01 00:00:00

  • Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants.

    abstract：:Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2005.02.007

    authors： Zhang T,Isayeva A,Adams SL,Rawson DM

    更新日期：2005-06-01 00:00:00

  • Freezability of Iberian ibex (Capra pyrenaica) spermatozoa according to the glycerolization temperature and plasma testosterone concentration.

    abstract：:Ibex spermatozoa can be successfully frozen using glycerolated media. However, no information is available regarding the most effective method of glycerol addition in this species. The aim of the present work was to evaluate the effect of the glycerolization temperature on the response to freezing-thawing of ibex sper...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2010.07.005

    authors： Coloma MA,Gómez-Brunet A,Velázquez R,Toledano-Díaz A,López-Sebastián A,Santiago-Moreno J

    更新日期：2010-10-01 00:00:00

  • Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli.

    abstract：:Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyo...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.07.012

    authors： Lavenburg VM,Liao YT,Salvador A,Hsu AL,Harden LA,Wu VCH

    更新日期：2020-10-01 00:00:00

  • The effects of cryopreservation and cold storage on sperm subpopulation structure of common carp (Cyprinus carpio L.).

    abstract：:The objectives of this study were to identify the presence of different spermatozoa subpopulations (SPs) according to their kinematic characteristics in the sperm of common carp and to test the effects of cryopreservation and prolonged (6-day) storage at room temperature (RT; 23 °C) and 4 °C on spermatozoa motility an...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2021.01.007

    authors： Marinović Z,Šćekić I,Lujić J,Urbányi B,Horváth Á

    更新日期：2021-01-12 00:00:00

  • An experimental study on periodontal regeneration after subcutaneous transplantation of rat molar with and without cryopreservation: an in vivo study.

    abstract：:This study analysed the effects of cryopreservation on periodontal regeneration of autotransplanted rat molars. First and second maxillary molars (n=92) of 24 four-week-old Wistar rats were gently extracted and autotransplanted into the abdominal tissue immediately (control group n=44) or after cryopreservation in liq...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.03.008

    authors： Staels S,De Coster P,Vral A,Temmerman L,De Pauw G

    更新日期：2013-06-01 00:00:00

  • Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids.

    abstract：:Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years posses...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.08.001

    authors： Lee S,Iwasaki Y,Yoshizaki G

    更新日期：2016-10-01 00:00:00

  • Sensitivity of thermophysiological models of cryoablation to the thermal and biophysical properties of tissues.

    abstract：:Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.10.006

    authors： Chan JY,Ooi EH

    更新日期：2016-12-01 00:00:00

  • An amino acidic adjuvant to augment cryoinjury of MCF-7 breast cancer cells.

    abstract：:One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.05.007

    authors： Wang CL,Teo KY,Han B

    更新日期：2008-08-01 00:00:00

  • Effect of cryoprotectants on the viability and function of unfrozen human polymorphonuclear cells.

    abstract：:High concentrations of membrane permeable cryoprotectants are necessary to protect human polymorphonuclear leukocytes from osmotic stress injury during freezing, but there are reports that some cryoprotectants are chemically toxic. Cells were exposed to various concentrations of glycerol, dimethyl sulfoxide, or ethyle...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(85)90181-6

    authors： Takahashi T,Bross JB,Shaber RE,Williams RJ

    更新日期：1985-08-01 00:00:00

  • The state of T cells before cryopreservation: Effects on post-thaw proliferation and function.

    abstract：AIM:We aim to assess the effect of the state of T cells before cryopreservation on the post-thaw proliferative capacity, phenotype and functional response. METHODS:Peripheral blood mononuclear cells (PBMCs) were isolated from a hepatocellular carcinoma (HCC) patient, and the T cells were frozen during cell culture acc...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2017.08.008

    authors： Luo Y,Wang P,Liu H,Zhu Z,Li C,Gao Y

    更新日期：2017-12-01 00:00:00

  • Fc receptors and human T lymphocytes in frozen-thawed peripheral blood cells.

    abstract：:The present study was carried out to investigate the influence of cryopreservation on human T-cell subsets defined by their membrane receptors for Fc IgM (TM) and Fc IgG (TG) and by their membrane antigens. For this purpose isolated T cells, obtained by neuraminidase-treated sheep erythrocyte (E-N) rosetting, and enri...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(84)90080-4

    authors： Pusztai-Markos Z,Ebenfeld R,Scheiwe MW

    更新日期：1984-08-01 00:00:00

  • Thermal study of simple amino-alcohol solutions.

    abstract：:Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/s0011-2240(02)00017-2

    authors： Baudot A,Cacela C,Duarte ML,Fausto R

    更新日期：2002-04-01 00:00:00

  • Cryopreservation of turkey semen: effect of breeding line and freezing method on post-thaw sperm quality, fertilization, and hatching.

    abstract：:Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw function of sperm from fi...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.04.003

    authors： Long JA,Purdy PH,Zuidberg K,Hiemstra SJ,Velleman SG,Woelders H

    更新日期：2014-06-01 00:00:00

  • Spontaneous activity of preoptic neurons in slice preparations of the hypothalamus of European hamsters (Cricetus cricetus) and Wistar rats under different states of hypothermia.

    abstract：:Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2122

    authors： Hashimoto M,Schmid HA,Ohwatari N,Pleschka K

    更新日期：1998-11-01 00:00:00

  • Vitrification of turbot embryos: preliminary assays.

    abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/s0011-2240(03)00066-x

    authors： Robles V,Cabrita E,Real M,Alvarez R,Herráez MP

    更新日期：2003-08-01 00:00:00

  • Comparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezing.

    abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.09.001

    authors： Yildiz C,Mullen B,Jarvi K,McKerlie C,Lo KC

    更新日期：2018-10-01 00:00:00

  • Cryoprotectant redistribution along the frozen straw probed by Raman spectroscopy.

    abstract：:The distribution of cryoprotectant (10% glycerol) and ice along the frozen plastic straw (the most useful container for freezing mammalian semen, oocytes and embryos) was studied by Raman scattering technique. Raman spectroscopy being a contactless, non-invasive tool was applied for the straws filled with the cryoprot...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.01.002

    authors： Karpegina YA,Okotrub KA,Brusentsev EY,Amstislavsky SY,Surovtsev NV

    更新日期：2016-04-01 00:00:00

  • Improved low-CPA vitrification of mouse oocytes using quartz microcapillary.

    abstract：:Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2015.04.003

    authors： Choi JK,Huang H,He X

    更新日期：2015-06-01 00:00:00

  • Renal impairment in hepatic cryotherapy.

    abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2085

    authors： Bagia JS,Perera DS,Morris DL

    更新日期：1998-06-01 00:00:00

  • Restoration of resistance to osmotic swelling of vitrified mouse embryos by short-term culture.

    abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1999.2163

    authors： Edashige K,Asano A,An TZ,Kasai M

    更新日期：1999-06-01 00:00:00