Abstract：
:Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyophilization with different cryoprotectants (sucrose and trehalose) and concentrations (0.1 M and 0.5 M) on the stability of seven lytic phages specific to STEC O157 and top 6 non-O157 strains during 6-month storage at -80 °C. The titers of lyophilized phages specific to STEC O26 (S1 O26) and STEC O121 (Pr121lvw) did not exhibit significant reduction after 6-month storage regardless of the use of cryoprotectants. Phages lytic against STEC O103 (Ro103C3lw) and STEC O145 (Ro145clw) with 0.1 M sucrose retained similar titers after lyophilization and frozen storage for 6 months (P > 0.05). Despite subtle differences, these results indicated that most of the selected phages had similar titer retention with the same cryoprotectants. Additionally, lytic activities of the phages against their primary hosts were not affected after lyophilization and 6-month frozen storage. Moreover, no detectable damage was observed on the lyophilized phage structures. These findings provide valuable insight into the use of lyophilization to preserve phages lytic against STEC strains.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Lavenburg VM,Liao YT,Salvador A,Hsu AL,Harden LA,Wu VCHdoi
10.1016/j.cryobiol.2020.07.012subject
Has Abstractpub_date
2020-10-01 00:00:00pages
85-91eissn
0011-2240issn
1090-2392pii
S0011-2240(20)30243-1journal_volume
96pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：:The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.04.002
更新日期：2014-06-01 00:00:00
abstract：:Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the cult...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.10.011
更新日期：2015-02-01 00:00:00
abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.06.009
更新日期：2017-10-01 00:00:00
abstract：:Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.10.017
更新日期：2020-11-04 00:00:00
abstract：:Our objective was to study the effect of the concentration of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO) during vitrification on the development of porcine blastocysts. Vitrification was performed with 0.4 M sucrose and either a Me2SO and EG mixture (15%, 16% and 17% v/v of each) or EG alone (40% v/v), using ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.02.005
更新日期：2008-06-01 00:00:00
abstract：:As known for different metabolic functions, α-lipoic acid (ALA) has been tested for spermatozoa preservation of animals as well as of human, but not for fish spermatozoa. The present study determined the effects of ALA on short and long-term (cryopreservation) preservation of common carp (Cyprinus carpio) spermatozoa,...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.04.006
更新日期：2020-06-01 00:00:00
abstract：:The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.08.005
更新日期：2013-12-01 00:00:00
abstract：:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.06.003
更新日期：2014-08-01 00:00:00
abstract：AIM:We aim to assess the effect of the state of T cells before cryopreservation on the post-thaw proliferative capacity, phenotype and functional response. METHODS:Peripheral blood mononuclear cells (PBMCs) were isolated from a hepatocellular carcinoma (HCC) patient, and the T cells were frozen during cell culture acc...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.08.008
更新日期：2017-12-01 00:00:00
abstract：:An insect antifreeze protein gene Mpafp698 was cloned by the RT-PCR approach from the desert beetle Microdera punctipennis. The gene was constructed and heterogeneously expressed in Escherichia coli as fusion proteins, His-MpAFP698, glutathione S-transferase (GST)-MpAFP698, and maltose-binding protein (MBP)-MpAFP698. ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.10.014
更新日期：2010-04-01 00:00:00
abstract：:The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. This study on the Anuran amphibian (B...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2129
更新日期：1998-12-01 00:00:00
abstract：:Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2004.01.010
更新日期：2004-06-01 00:00:00
abstract：:The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2011.12.001
更新日期：2012-04-01 00:00:00
abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.11.015
更新日期：2020-12-04 00:00:00
abstract：:The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/s0011-2240(03)00045-2
更新日期：2003-06-01 00:00:00
abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(91)90026-k
更新日期：1991-06-01 00:00:00
abstract：:The effect of cryopreservation on human nasal mucosal biopsies was evaluated by determining the frequency and coordination of the ciliary beat. Samples were cryopreserved in a medium containing 80% Gey's balanced salt solution, 10% dimethyl sulfoxide, and 10% fetal calf serum. After thawing, the samples were put in a ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(85)90164-6
更新日期：1985-04-01 00:00:00
abstract：:Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2016.10.006
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:The effect of dimethyl sulfoxide (Me2SO) on enumeration of post-thaw CD45+ and CD34+ cells of umbilical cord blood (HPC-C) and mobilized peripheral blood (HPC-A) has not been systematically studied. METHODS:Cells from leukapheresis products from multiple myeloma patients and umbilical cord blood cells were ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.06.003
更新日期：2005-10-01 00:00:00
abstract：:The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.09.010
更新日期：2008-12-01 00:00:00
abstract：:Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2227
更新日期：2000-03-01 00:00:00
abstract：:The cause of slow freezing injury and the basis of the protection by solutes like glycerol are subjects of debate. During slow freezing, cells are sequestered in unfrozen channels between ice crystals that grow by removing pure water from the channels. As a consequence, the solute concentration in the channels rises a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90030-8
更新日期：1989-02-01 00:00:00
abstract：:The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2221
更新日期：2000-02-01 00:00:00
abstract：:To evaluate the effect of Ca2+ on recovery of heart function after hypothermic preservation, isolated working rat hearts were perfused continuously in different [Ca2+] (0.35-2.0 mM) at 37 degrees C for 30 min, during cooling to 5 degrees C, and then at 5 degrees C for 1, 6 or 18 h. After rewarming to 37 degrees C, con...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1994.1031
更新日期：1994-06-01 00:00:00
abstract：:Percutaneous ablation is the currently preferred locoregional therapy for non-resectable hepatocellular cancer (HCC). Cryoablation is an attractive option because it forms an ice ball viewable by many imaging methods. This study assessed the therapeutic effect of comprehensive cryoablation (of intra- and extrahepatic ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2012.11.008
更新日期：2013-02-01 00:00:00
abstract：:The ability to routinely cryopreserve micro-algal species reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift. Cryopreservation is also a useful adjunct in aquaculture hatcheries for strains of micro-a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.10.003
更新日期：2006-02-01 00:00:00
abstract：:The development of drugs in cardiovascular research involves in vitro studies that are generally performed on freshly isolated blood vessels. Naturally, data obtained from human tissues would be the most accurate and predictable for human pharmacology. However, the main problems are that the supply of fresh human mate...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1006/cryo.1994.1007
更新日期：1994-02-01 00:00:00
abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.09.001
更新日期：2018-10-01 00:00:00
abstract：:Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver p...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.10.003
更新日期：2018-12-01 00:00:00
abstract：:Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.08.007
更新日期：2008-12-01 00:00:00
