Frequency and coordination of ciliary beat after cryopreservation of respiratory epithelium.

abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...

journal_title：Cryobiology

authors： Izamis ML,Perk S,Calhoun C,Uygun K,Yarmush ML,Berthiaume F

更新日期：2015-10-01 00:00:00

abstract：:Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibili...

journal_title：Cryobiology

authors： Niknejad H,Deihim T,Solati-Hashjin M,Peirovi H

更新日期：2011-12-01 00:00:00

abstract：:Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...

journal_title：Cryobiology

authors： Warner RM,Ampo E,Nelson D,Benson JD,Eroglu A,Higgins AZ

更新日期：2020-11-04 00:00:00

abstract：:Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...

journal_title：Cryobiology

authors： Du Y,Zhang Y,Li J,Kragh PM,Kuwayama M,Ieda S,Zhang X,Schmidt M,Bøgh IB,Purup S,Pedersen AM,Villemoes K,Yang H,Bolund L,Vajta G

更新日期：2007-04-01 00:00:00

abstract：:The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice-nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleatin...

journal_title：Cryobiology

authors： Weng L,Tessier SN,Swei A,Stott SL,Toner M

更新日期：2017-04-01 00:00:00

abstract：PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...

journal_title：Cryobiology

authors： Herbemont C,Mnallah S,Bennani-Smires B,Peigne M,Cedrin-Durnerin I,Grynberg M,Sifer C

更新日期：2021-01-02 00:00:00

abstract：:The exchange of genetically engineered mouse strains between research facilities requires transporting fresh mouse sperm under refrigerated temperatures. Although sperm generally maintains fertility for 48 h at cold temperatures, in vitro fertilization rates of C57BL/6 mouse sperm are low after 48-h cold storage. Furt...

journal_title：Cryobiology

authors： Takeo T,Tsutsumi A,Omaru T,Fukumoto K,Haruguchi Y,Kondo T,Nakamuta Y,Takeshita Y,Matsunaga H,Tsuchiyama S,Sakoh K,Nakao S,Yoshimoto H,Shimizu N,Nakagata N

更新日期：2012-12-01 00:00:00

abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

journal_title：Cryobiology

authors： Biggar KK,Dubuc A,Storey K

更新日期：2009-12-01 00:00:00

abstract：:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facil...

journal_title：Cryobiology

authors： Caruana M,Battle T,Fuller B,Davidson B

更新日期：1999-03-01 00:00:00

abstract：:Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the cult...

journal_title：Cryobiology

authors： Del Pino A,Ligero G,López MB,Navarro H,Carrillo JA,Pantoll SC,Díaz de la Guardia R

更新日期：2015-02-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:The development of efficient and robust methods for the cryopreservation of human embryonic stem cells (hESCs) is important for the production of master and working cell banks for future clinical applications. Such methods must meet requirements of good manufacturing practice (GMP) and maintain genetic stability of th...

journal_title：Cryobiology

authors： Amps KJ,Jones M,Baker D,Moore HD

更新日期：2010-06-01 00:00:00

abstract：:Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...

journal_title：Cryobiology

authors： Woods EJ,Liu J,Gilmore JA,Reid TJ,Gao DY,Critser JK

更新日期：1999-05-01 00:00:00

abstract：:As known for different metabolic functions, α-lipoic acid (ALA) has been tested for spermatozoa preservation of animals as well as of human, but not for fish spermatozoa. The present study determined the effects of ALA on short and long-term (cryopreservation) preservation of common carp (Cyprinus carpio) spermatozoa,...

journal_title：Cryobiology

authors： İnanan BE,Kanyılmaz M

更新日期：2020-06-01 00:00:00

abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...

journal_title：Cryobiology

authors： Hunter JE,Bernard A,Fuller BJ,McGrath JJ,Shaw RW

更新日期：1992-04-01 00:00:00

abstract：:The present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and L...

journal_title：Cryobiology

authors： Kocyigit A,Cevik M

更新日期：2015-08-01 00:00:00

abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...

journal_title：Cryobiology

authors： Yu SO,Brown A,Middleton AJ,Tomczak MM,Walker VK,Davies PL

更新日期：2010-12-01 00:00:00

abstract：:Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...

journal_title：Cryobiology

authors： Anil S,Rawson D,Zhang T

更新日期：2018-08-01 00:00:00

abstract：:Cryopreservation of tissue engineered bone (TEB), whilst maintaining its osteogenic ability, is imperative for large-scale clinical application. We previously reported a novel cell transplantation method, in which bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured to confluence and differentiated down th...

journal_title：Cryobiology

authors： Shimizu T,Akahane M,Ueha T,Kido A,Omokawa S,Kobata Y,Murata K,Kawate K,Tanaka Y

更新日期：2013-06-01 00:00:00

abstract：:Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...

journal_title：Cryobiology

authors： Choi JK,Huang H,He X

更新日期：2015-06-01 00:00:00

abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...

journal_title：Cryobiology

authors： Edashige K,Asano A,An TZ,Kasai M

更新日期：1999-06-01 00:00:00

abstract：:Twenty-eight-day-old male rats were used in three experiments to study whether cold exposure potentiates pineal actions in nonhibernating mammals. The following questions were considered: (a) Can cold exposure increase the antigonadal effects of light deprivation? (b) Are the effects induced by blindness plus cold exp...

journal_title：Cryobiology

authors： Sánchez-Barceló EJ,Cos S,Mediavilla MD

更新日期：1986-02-01 00:00:00

abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...

journal_title：Cryobiology

authors： Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

更新日期：2020-12-04 00:00:00

abstract：:Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/...

journal_title：Cryobiology

authors： Tsuji M,Goshima T,Matsushika A,Kudoh S,Hoshino T

更新日期：2013-10-01 00:00:00

abstract：:The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...

journal_title：Cryobiology

authors： Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

更新日期：2001-11-01 00:00:00

abstract：:Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disacchari...

journal_title：Cryobiology

authors： Chakraborty N,Menze MA,Elmoazzen H,Vu H,Yarmush ML,Hand SC,Toner M

更新日期：2012-04-01 00:00:00

abstract：:Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

journal_title：Cryobiology

authors： Karow AM Jr,Jeske AH

更新日期：1976-08-01 00:00:00

abstract：:The effect of cryopreservation on ciliary activity of human nasal respiratory epithelium was evaluated. Samples were cryopreserved in a solution containing nutrient medium, 10% fetal calf serum, and two different concentrations (10 or 20%) of dimethyl sulfoxide and stored in liquid nitrogen at -196 degrees C for 2 wee...

journal_title：Cryobiology

authors： Di Benedetto G,Gill J,Lopez-Vidriero MT,Clarke SW

更新日期：1989-08-01 00:00:00

abstract：:Storing spores is a promising method to conserve genetic diversity of ferns ex situ. Inappropriate water contents or damaging effects of triacylglycerol (TAG) crystallization may cause initial damage and deterioration with time in spores placed at -15 degrees C or liquid nitrogen temperatures. We used differential sca...

journal_title：Cryobiology

authors： Ballesteros D,Walters C

更新日期：2007-08-01 00:00:00