当前位置： SCI文献检索 > CRYOBIOLOGY期刊下所有文献 > Simplified cryopreservation of porcine cloned blastocysts.

Simplified cryopreservation of porcine cloned blastocysts.

Abstract：

:Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC) - to establish a simplified and efficient cryopreservation system for porcine cloned embryos. In Experiment 1, zonae pellucidae of oocytes were partially digested with pronase, followed by centrifugation to polarize lipid particles. Ninety percent (173/192) oocytes were successfully delipated in this way. Parthenogenetic activation (PA) after complete removal of zona resulted in similar blastocyst rates in delipated vs. control oocytes (28+/-7% vs. 28+/-5%, respectively). Subsequent vitrification of produced blastocysts with the Cryotop technique resulted in higher survival rates in the delipated group compared to the control group (85+/-6% vs. 32+/-7%, respectively; P<0.01). In Experiment 2, delipated oocytes were used for HMC with normal oocytes as control. Partial zona digestion was further applied before enucleation both in delipated and control groups, to bisect oocyte successfully. Although the blastocyst rate of reconstructed embryos was similar between groups derived from delipated vs. control oocytes (21+/-6% and 23+/-6%, respectively), after vitrification higher survival rates were achieved in the delipated groups than in controls (79+/-6% vs. 32+/-8%, respectively). Our results prove that porcine embryos produced from delipated oocytes by PA or HMC can be cryopreserved effectively by ultrarapid vitrification. Further experiments are required to assess the in vivo developmental competence of the cloned-vitrified embryos.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Du Y,Zhang Y,Li J,Kragh PM,Kuwayama M,Ieda S,Zhang X,Schmidt M,Bøgh IB,Purup S,Pedersen AM,Villemoes K,Yang H,Bolund L,Vajta G

doi

10.1016/j.cryobiol.2007.01.001

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

181-7

issue

2

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(07)00004-1

journal_volume

54

pub_type

杂志文章

相关文献

CRYOBIOLOGY文献大全
  • Visualization of intracellular ice formation using high-speed video cryomicroscopy.

    abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.11.003

    authors： Stott SL,Karlsson JOM

    更新日期：2009-02-01 00:00:00

  • Use of dynamic mechanical analysis (DMA) to determine critical transition temperatures in frozen biomaterials intended for lyophilization.

    abstract：:Dynamic mechanical analysis is widely used to determine glass transitions in solid state materials. However, here we demonstrate the application of DMA for the determination of glass transitions (Tg') in the frozen liquid state by means of a steel sample pocket. The use of the pocket allows frozen material to be analy...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2010.04.002

    authors： Gearing J,Malik KP,Matejtschuk P

    更新日期：2010-08-01 00:00:00

  • Non-disaccharide-based mechanisms of protection during drying.

    abstract：:Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of dis...

    journal_title：Cryobiology

    pub_type： 杂志文章,评审

    doi：10.1006/cryo.2001.2359

    authors： Oliver AE,Leprince O,Wolkers WF,Hincha DK,Heyer AG,Crowe JH

    更新日期：2001-09-01 00:00:00

  • Long-term preservation of dried phosphofructokinase by sugars and sugar/zinc mixtures.

    abstract：:We have demonstrated that sugars and suger/zinc mixtures can be used to preserve the activity of dried phosphofructokinase (PFK) during long-term storage over CaSO4. After 9 weeks in the presence of either 200 mM sucrose or 200 mM trehalose little loss of PFK activity was noted, with almost 60% of the original prefree...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(88)90045-4

    authors： Carpenter JF,Martin B,Loomis SH,Crowe JH

    更新日期：1988-08-01 00:00:00

  • Direct ethanol fermentation from lignocellulosic biomass by Antarctic basidiomycetous yeast Mrakia blollopis under a low temperature condition.

    abstract：:Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.06.003

    authors： Tsuji M,Goshima T,Matsushika A,Kudoh S,Hoshino T

    更新日期：2013-10-01 00:00:00

  • Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen.

    abstract：:This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2017.03.002

    authors： Seifi-Jamadi A,Ahmad E,Ansari M,Kohram H

    更新日期：2017-04-01 00:00:00

  • Linking membrane physical properties and low temperature tolerance in arthropods.

    abstract：:Maintenance of membrane fluidity is of crucial importance in ectotherms experiencing thermal changes. This maintenance has in ectotherms most often been indicated using indirect measures of biochemical changes of phospholipid membranes, which is then assumed to modulate the physico-chemical properties of the membrane....

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.09.164

    authors： Waagner D,Bouvrais H,Ipsen JH,Holmstrup M

    更新日期：2013-12-01 00:00:00

  • Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids.

    abstract：:Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years posses...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.08.001

    authors： Lee S,Iwasaki Y,Yoshizaki G

    更新日期：2016-10-01 00:00:00

  • Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing.

    abstract：:Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/s0011-2240(02)00181-5

    authors： Hunt CJ,Armitage SE,Pegg DE

    更新日期：2003-02-01 00:00:00

  • Treatment with cholesterol-loaded methyl-β-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes.

    abstract：:Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol h...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.05.006

    authors： Jiménez-Trigos E,Vicente JS,Mocé E,Naturil-Alfonso C,Fernandez-Gonzalez R,Gutierrez-Adan A,Marco-Jiménez F

    更新日期：2013-08-01 00:00:00

  • A re-evaluation of the role of type IV antifreeze protein.

    abstract：:A lipoprotein-like antifreeze protein (type IV AFP) has previously been isolated only from the blood plasma of the longhorn sculpin. However, the plasma antifreeze activity in all individuals of this species tested from Newfoundland and New Brunswick waters ranges from low to undetectable. A close relative of the long...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.10.122

    authors： Gauthier SY,Scotter AJ,Lin FH,Baardsnes J,Fletcher GL,Davies PL

    更新日期：2008-12-01 00:00:00

  • Ex-vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers.

    abstract：:Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2004.01.010

    authors： Jain S,Xu H,Duncan H,Jones JW Jr,Zhang JX,Clemens MG,Lee CY

    更新日期：2004-06-01 00:00:00

  • Protective effects of osmolytes in cryopreserving adherent neuroblastoma (Neuro-2a) cells.

    abstract：:A simple method to cryopreserve adherent monolayers of neuronal cells is currently not available, but the development of this technique could facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. However, complex tissues of some exceptional animals survive ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2015.08.015

    authors： Bailey TL,Wang M,Solocinski J,Nathan BP,Chakraborty N,Menze MA

    更新日期：2015-12-01 00:00:00

  • Application of the osmotic virial equation in cryobiology.

    abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.07.011

    authors： Prickett RC,Elliott JA,McGann LE

    更新日期：2010-02-01 00:00:00

  • Thermally defined cryomicroscopy and thermodynamic analysis in lymphocyte freezing.

    abstract：:A cryomicroscope is described which provides the possibility of quantifying the volume loss of cells during freezing, detection of intracellular ice formation during cooling and warming, as well as the determination of viability as function of (constant) cooling rates. The basic mechanisms occurring in cryopreservatio...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(84)90026-9

    authors： Scheiwe MW,Körber C

    更新日期：1984-02-01 00:00:00

  • Glass-forming tendency and stability of the amorphous state in the aqueous solutions of linear polyalcohols with four carbons. I. Binary systems water-polyalcohol.

    abstract：:All the aqueous solutions of linear saturated polyalcohols with four carbons have been investigated at low temperature. Only ice has been observed in the solutions of 1,3-butanediol and 1,2,3- and 1,2,4-butanetriol. For same solute concentration, the glass-forming tendency on cooling is highest with 2,3-butanediol, wh...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(86)90031-3

    authors： Boutron P,Mehl P,Kaufmann A,Angibaud P

    更新日期：1986-10-01 00:00:00

  • Viral contamination of embryos cryopreserved in liquid nitrogen.

    abstract：:Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1999.2227

    authors： Bielanski A,Nadin-Davis S,Sapp T,Lutze-Wallace C

    更新日期：2000-03-01 00:00:00

  • Platelet cryopreservation with glycerol, dextran, and mannitol: recovery of 5-hydroxytryptamine uptake and hypotonic stress response.

    abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(85)90002-1

    authors： Brodthagen UA,Armitage WJ,Parmar N

    更新日期：1985-02-01 00:00:00

  • Thermal stability properties of an antifreeze protein from the desert beetle Microdera punctipennis.

    abstract：:An insect antifreeze protein gene Mpafp698 was cloned by the RT-PCR approach from the desert beetle Microdera punctipennis. The gene was constructed and heterogeneously expressed in Escherichia coli as fusion proteins, His-MpAFP698, glutathione S-transferase (GST)-MpAFP698, and maltose-binding protein (MBP)-MpAFP698. ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.10.014

    authors： Qiu LM,Ma J,Wang J,Zhang FC,Wang Y

    更新日期：2010-04-01 00:00:00

  • Functionality of cryopreserved juvenile ovaries from mutant mice in different genetic background strains after allotransplantation.

    abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.10.003

    authors： Huang KY,de Groot SA,Woelders H,van der Horst GT,Themmen AP,Colenbrander B,van Vlissingen JM

    更新日期：2010-04-01 00:00:00

  • A novel strategy for conservation of European eel (Anguilla anguilla) genetic resources: Cryopreservation of ovarian stem cells.

    abstract：:The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was de...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.03.009

    authors： Šćekić I,Marinović Z,Lujić J,Müller T,Kitanović N,Urbányi B,Horváth Á

    更新日期：2020-08-01 00:00:00

  • Short- and long-term freezing effects in a coastal (Lobaria virens) versus a widespread lichen (L. pulmonaria).

    abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.03.007

    authors： Solhaug KA,Chowdhury DP,Gauslaa Y

    更新日期：2018-06-01 00:00:00

  • A 9 kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni.

    abstract：:A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.07.001

    authors： Hawes TC,Marshall CJ,Wharton DA

    更新日期：2014-08-01 00:00:00

  • Comparative analysis of the transcriptome of the overwintering desert beetle Microdera punctipennis.

    abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2017.06.009

    authors： Tusong K,Guo X,Meng S,Liu X,Ma J

    更新日期：2017-10-01 00:00:00

  • Laboratory and animal model evaluation of the Cryotech LCS 2000 in hepatic cryotherapy.

    abstract：:Hepatic cryotherapy is increasingly used in the treatment of patients with multiple hepatic metastases, particularly from colorectal cancer. The Cryotech LCS 2000 system, with insulated shaft-circulated liquid nitrogen probes, was designed for this purpose and was evaluated on the bench and in an animal model. The 9-m...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1993.1007

    authors： Dilley AV,Dy DY,Warlters A,Copeland S,Gillies AE,Morris RW,Gibb DB,Cook TA,Morris DL

    更新日期：1993-02-01 00:00:00

  • Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide.

    abstract：:Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use. However, one major obstacle to the manufacturing of clinical grade stem cells is the need for current good manufacturing practice...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2011.02.003

    authors： Seo JM,Sohn MY,Suh JS,Atala A,Yoo JJ,Shon YH

    更新日期：2011-06-01 00:00:00

  • Hatching, survival and deformities of piracanjuba (Brycon orbignyanus) embryos subjected to different cooling protocols.

    abstract：:Groups of one hundred Brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. Different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (Me2SO); at different temperatures (0.0±2.0°C and 8.0±2....

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.10.003

    authors： Paes Mdo C,da Silva RC,do Nascimento NF,Valentin FN,Senhorini JA,Nakaghi LS

    更新日期：2014-12-01 00:00:00

  • New approaches for studying the permeability of fish embryos: toward successful cryopreservation.

    abstract：:This paper describes some new approaches for understanding the permeability of teleost embryos. The dechorionated zebrafish (Brachydanio rerio) was used as a model for basic studies of water and cryoprotectant permeability. These embryos are composed of two compartments, a large yolk (surrounded by the yolk syncytial ...

    journal_title：Cryobiology

    pub_type： 杂志文章,评审

    doi：10.1006/cryo.1997.2014

    authors： Hagedorn M,Hsu E,Kleinhans FW,Wildt DE

    更新日期：1997-06-01 00:00:00

  • Protection from nonfreezing cold injury by quinacrine, a phospholipase inhibitor.

    abstract：:A recent study from our laboratory indicated additional tissue injury during rewarming of a cooled rabbit leg. Oxygen-derived free radicals were believed to play a role in such "rewarming injury." Since free radicals may attack membrane phospholipids, we analyzed the phospholipid composition in the leg tissue during c...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(91)90020-o

    authors： Das DK,Iyengar J,Jones RM,Lu D,Maity S

    更新日期：1991-04-01 00:00:00

  • Effect of sugars in the preservation solution on liver storage in rats.

    abstract：:We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(91)90051-o

    authors： Kobayashi T,Sumimoto R,Shimada H,Kamada N,Nakagawara G

    更新日期：1991-10-01 00:00:00