The effect of cryopreservation on ciliary beat frequency of human respiratory epithelium.


:The effect of cryopreservation on ciliary activity of human nasal respiratory epithelium was evaluated. Samples were cryopreserved in a solution containing nutrient medium, 10% fetal calf serum, and two different concentrations (10 or 20%) of dimethyl sulfoxide and stored in liquid nitrogen at -196 degrees C for 2 weeks. Ciliary beat frequencies (CBF) of the samples before and after cryopreservation were compared. Mean CBF values did not differ significantly with both concentrations of dimethyl sulfoxide. The mean intrasample coefficient of variation of the CBF decreased significantly after cryopreservation. After thawing, CBF remained unchanged for at least 4 hr. It is concluded that normal ciliated epithelial cells can be frozen and stored in liquid nitrogen at 196 degrees C while maintaining their CBF.






Di Benedetto G,Gill J,Lopez-Vidriero MT,Clarke SW




Has Abstract


1989-08-01 00:00:00














  • Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide.

    abstract::Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use. However, one major obstacle to the manufacturing of clinical grade stem cells is the need for current good manufacturing practice...


    pub_type: 杂志文章


    authors: Seo JM,Sohn MY,Suh JS,Atala A,Yoo JJ,Shon YH

    更新日期:2011-06-01 00:00:00

  • Improved stability of live attenuated vaccine gdhA derivative Pasteurella multocida B:2 by freeze drying method for use as animal vaccine.

    abstract::The efficacy of attenuated strain of gdhA derivative Pasteurella multocida B:2 mutant as a live vaccine to control haemorrhagic septicaemia (HS) disease in cattle and buffaloes has been demonstrated. In order to use P. multocida B:2 mutant as a commercial product, it is essential to optimise its formulation for high v...


    pub_type: 杂志文章


    authors: Oslan SNH,Halim M,Ramle NA,Saad MZ,Tan JS,Kapri MR,Ariff AB

    更新日期:2017-12-01 00:00:00

  • Dependence of trehalose protective action on the initial phase state of dipalmitoylphosphatidylcholine bilayers.

    abstract::Dipalmitoylphosphatidylcholine (DPPC) bilayers hydrated in the presence of trehalose were equilibrated at various temperatures (4, 20, and 60 degrees C) corresponding to the crystalline Lc, gel L beta', and liquid-crystalline L alpha phases, respectively, and then desiccated at these temperatures or freeze-dried at -8...


    pub_type: 杂志文章


    authors: Tsvetkova N,Tenchov B,Tsonev L,Tsvetkov T

    更新日期:1988-06-01 00:00:00

  • A 9 kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni.

    abstract::A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...


    pub_type: 杂志文章


    authors: Hawes TC,Marshall CJ,Wharton DA

    更新日期:2014-08-01 00:00:00

  • Effect of alpha-lipoic acid on oxidative stress, viability and motility in the common carp (Cyprinus carpio) spermatozoa after short-term storage and cryopreservation.

    abstract::As known for different metabolic functions, α-lipoic acid (ALA) has been tested for spermatozoa preservation of animals as well as of human, but not for fish spermatozoa. The present study determined the effects of ALA on short and long-term (cryopreservation) preservation of common carp (Cyprinus carpio) spermatozoa,...


    pub_type: 杂志文章


    authors: İnanan BE,Kanyılmaz M

    更新日期:2020-06-01 00:00:00

  • Improved low-CPA vitrification of mouse oocytes using quartz microcapillary.

    abstract::Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...


    pub_type: 杂志文章


    authors: Choi JK,Huang H,He X

    更新日期:2015-06-01 00:00:00

  • A comparison of sucrose, saline, and saline with egg-yolk diluents on the cryopreservation of cane toad (Bufo marinus) sperm.

    abstract::Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...


    pub_type: 杂志文章


    authors: Browne RK,Mahony M,Clulow J

    更新日期:2002-06-01 00:00:00

  • An amino acidic adjuvant to augment cryoinjury of MCF-7 breast cancer cells.

    abstract::One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...


    pub_type: 杂志文章


    authors: Wang CL,Teo KY,Han B

    更新日期:2008-08-01 00:00:00

  • Spontaneous activity of preoptic neurons in slice preparations of the hypothalamus of European hamsters (Cricetus cricetus) and Wistar rats under different states of hypothermia.

    abstract::Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 ...


    pub_type: 杂志文章


    authors: Hashimoto M,Schmid HA,Ohwatari N,Pleschka K

    更新日期:1998-11-01 00:00:00

  • Vitrification properties of solutions of ethylene glycol in saline containing PVP, Ficoll, or dextran.

    abstract::Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...


    pub_type: 杂志文章


    authors: Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

    更新日期:1997-11-01 00:00:00

  • Pre-grafting histological studies of skin grafts cryopreserved in α helix antarctic yeast oriented antifreeze peptide (Afp1m).

    abstract::A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...


    pub_type: 杂志文章


    authors: Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

    更新日期:2020-02-01 00:00:00

  • Effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (Cyprinus carpio) sperm.

    abstract::Addition of cholesterol-loaded cyclodextrin (CLC) to the diluents of mammalian semen increased stability and rigidity of phospholipid hydrocarbon chains of plasma membrane during sperm cryopreservation process. CLC has been tested successfully as cryoprotectant in various livestock sperm cryopreservation protocols but...


    pub_type: 杂志文章


    authors: Yildiz C,Yavas I,Bozkurt Y,Aksoy M

    更新日期:2015-04-01 00:00:00

  • Effect of two cooling protocols on the post-thaw characteristics of Iberian ibex sperms.

    abstract::The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejacu...


    pub_type: 杂志文章


    authors: Esteso MC,Toledano-Díaz A,Castaño C,Pradiee J,Lopez-Sebastián A,Santiago-Moreno J

    更新日期:2018-02-01 00:00:00

  • Influence of cell concentration on the contribution of unfrozen fraction and salt concentration to the survival of slowly frozen human erythrocytes.

    abstract::We have shown previously that the survival of human red blood cells during slow freezing at 2% hematocrit is dependent more on the magnitude of the unfrozen fraction than on the salt concentration in that unfrozen fraction. In parallel, first Nei and more recently Pegg and colleagues have shown that survival is affect...


    pub_type: 杂志文章


    authors: Mazur P,Cole KW

    更新日期:1985-12-01 00:00:00

  • The effect of cooling rate and temperature on the toxicity of ethylene glycol in the rabbit internal carotid artery.

    abstract::The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...


    pub_type: 杂志文章


    authors: Wusteman M,Boylan S,Pegg DE

    更新日期:1996-08-01 00:00:00

  • Effects of cryopreservation on the immunogenicity of porcine arterial allografts in early stages of transplant vasculopathy.

    abstract:BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...


    pub_type: 杂志文章


    authors: Solanes N,Rigol M,Khabiri E,Castellà M,Ramírez J,Roqué M,Agustí E,Roig E,Pérez-Villa F,Segalés J,Pomar JL,Engel P,Massaguer A,Martorell J,Rodríguez JA,Sanz G,Heras M

    更新日期:2005-10-01 00:00:00

  • Short- and long-term freezing effects in a coastal (Lobaria virens) versus a widespread lichen (L. pulmonaria).

    abstract::Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...


    pub_type: 杂志文章


    authors: Solhaug KA,Chowdhury DP,Gauslaa Y

    更新日期:2018-06-01 00:00:00

  • Freezing monolayers of cells without gap junctions.

    abstract::Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...


    pub_type: 杂志文章


    authors: Armitage WJ,Juss BK

    更新日期:2003-04-01 00:00:00

  • Vitrification of in vitro produced goat blastocysts: effects of oocyte donor age and development stage.

    abstract::This study examines the effectiveness of the cryotop vitrification method for the cryopreservation of goat blastocysts. To determine the effects of embryo development stage and donor age on in vitro survival rates, good-quality blastocysts from adult and prepubertal goats were sorted into non-expanded, expanded, hatch...


    pub_type: 杂志文章


    authors: Morató R,Romaguera R,Izquierdo D,Paramio MT,Mogas T

    更新日期:2011-12-01 00:00:00

  • Little evidence for a major role of Ca2+ in cold-induced injury of liver cells.

    abstract::We have previously shown that cold-induced injury to hepatocytes and liver endothelial cells occurs predominantly via an iron-dependent pathway. However, other groups have reported evidences suggesting that Ca(2+) ions could be involved in the process of cold-induced injury of liver cells. We here assessed the relativ...


    pub_type: 杂志文章


    authors: Knoop S,de Groot H,Rauen U

    更新日期:2008-04-01 00:00:00

  • Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa.

    abstract::Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The pu...


    pub_type: 杂志文章


    authors: Hara H,Tagiri M,Hwang IS,Takahashi M,Hirabayashi M,Hochi S

    更新日期:2014-06-01 00:00:00

  • Physiological evaluation of a rabbit kidney perfused with VS41A.

    abstract::The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...


    pub_type: 杂志文章


    authors: Arnaud FG,Khirabadi B,Fahy GM

    更新日期:2003-06-01 00:00:00

  • Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing.

    abstract::Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...


    pub_type: 杂志文章


    authors: Hunt CJ,Armitage SE,Pegg DE

    更新日期:2003-02-01 00:00:00

  • Effects of vitrification on ram spermatozoa using free-egg yolk extenders.

    abstract::The present study aimed to examine the behavior of ram spermatozoa subjected to a vitrification process in free-egg yolk diluents in relation with conventional diluents and cryopreservation protocol used in this species. Previously it was investigated the toxicity of cryoprotectants, sucrose and glycerol, based on dif...


    pub_type: 杂志文章


    authors: Jiménez-Rabadán P,García-Álvarez O,Vidal A,Maroto-Morales A,Iniesta-Cuerda M,Ramón M,del Olmo E,Fernández-Santos R,Garde JJ,Soler AJ

    更新日期:2015-08-01 00:00:00

  • Effects of high-molecular-weight cryoprotectants on platelets and the coagulation system.

    abstract::The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...


    pub_type: 杂志文章


    authors: Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

    更新日期:2000-06-01 00:00:00

  • Effects of cell concentration on viability and metabolic activity during cryopreservation.

    abstract::Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...


    pub_type: 杂志文章


    authors: De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

    更新日期:1998-09-01 00:00:00

  • Galacto-oligosaccharides as protective molecules in the preservation of Lactobacillus delbrueckii subsp. bulgaricus.

    abstract::In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...


    pub_type: 杂志文章


    authors: Tymczyszyn EE,Gerbino E,Illanes A,Gómez-Zavaglia A

    更新日期:2011-04-01 00:00:00

  • Effectiveness of a tris-based extender (SHOTOR diluent) for the preservation of Bactrian camel (Camelus bactrianus) semen.

    abstract::The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...


    pub_type: 杂志文章


    authors: Niasari-Naslaji A,Mosaferi S,Bahmani N,Gharahdaghi AA,Abarghani A,Ghanbari A,Gerami A

    更新日期:2006-08-01 00:00:00

  • Fractures in cryopreserved arteries.

    abstract::The common carotid artery of the rabbit, a typical small elastic artery, can be cryopreserved using dimethyl sulfoxide, slow cooling, storage at less than -160 degrees C, and rapid warming. This technique provides satisfactory preservation of muscle and endothelial cells, but in about 75% of cases, gross circumferenti...


    pub_type: 杂志文章


    authors: Hunt CJ,Song YC,Bateson EA,Pegg DE

    更新日期:1994-10-01 00:00:00

  • A scale down process for the development of large volume cryopreservation.

    abstract::The process of ice formation and propagation during cryopreservation impacts on the post-thaw outcome for a sample. Two processes, either network solidification or progressive solidification, can dominate the water-ice phase transition with network solidification typically present in small sample cryo-straws or cryo-v...


    pub_type: 杂志文章


    authors: Kilbride P,Morris GJ,Milne S,Fuller B,Skepper J,Selden C

    更新日期:2014-12-01 00:00:00