Isolation of human hepatocytes after hepatic warm and cold ischemia: a practical approach using University of Wisconsin solution.

Abstract:

:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facilitate transfer of liver tissues between distant centers for cell isolation studies.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Caruana M,Battle T,Fuller B,Davidson B

doi

10.1006/cryo.1999.2155

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

165-8

issue

2

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(99)92155-7

journal_volume

38

pub_type

杂志文章
  • Characteristics and kinetics of subzero chilling injury in Drosophila embryos.

    abstract::Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(92)90005-m

    authors: Mazur P,Schneider U,Mahowald AP

    更新日期:1992-02-01 00:00:00

  • Magnetic resonance imaging-estimated three-dimensional temperature distribution in liver cryolesions: a study of cryolesion characteristics assumed necessary for tumor ablation.

    abstract::The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.2001.2351

    authors: Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

    更新日期:2001-11-01 00:00:00

  • Ezrin functionality and hypothermic preservation injury in LLC-PK1 cells.

    abstract::Renal epithelial cells from donor kidneys are susceptible to hypothermic preservation injury, which is attenuated when they over express the cytoskeletal linker protein ezrin. This study was designed to characterize the mechanisms of this protection. Renal epithelial cell lines were created from LLC-PK1 cells, which e...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2012.04.003

    authors: Tian T,Lindell SL,Lam M,Mangino MJ

    更新日期:2012-08-01 00:00:00

  • A fluorescence ratio-based method to determine microalgal viability and its application to rapid optimization of cryopreservation.

    abstract::The utility of microalgal biomass and bioproducts depends on long-term maintenance of certain physiological or biochemical features of the species. While unique characteristics may not be durably maintained with general subculture, cryopreservation methods better prevent alterations from desired characteristics. Post-...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2018.02.014

    authors: Zheng L,Lu Z,Zhang Q,Li T,Song L

    更新日期:2018-04-01 00:00:00

  • Fertilization and development of frozen-thawed germinal vesicle bovine oocytes by a one-step dilution method in vitro.

    abstract::The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown is particularly sensitive to cold and readily damaged by exposure to low temperatures, the da...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1996.0055

    authors: Suzuki T,Boediono A,Takagi M,Saha S,Sumantri C

    更新日期:1996-10-01 00:00:00

  • Sensitivity of thermophysiological models of cryoablation to the thermal and biophysical properties of tissues.

    abstract::Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2016.10.006

    authors: Chan JY,Ooi EH

    更新日期:2016-12-01 00:00:00

  • The effect of cooling rate and temperature on the toxicity of ethylene glycol in the rabbit internal carotid artery.

    abstract::The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1996.0042

    authors: Wusteman M,Boylan S,Pegg DE

    更新日期:1996-08-01 00:00:00

  • Osmotic parameters of red blood cells from umbilical cord blood.

    abstract::The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2014.04.002

    authors: Zhurova M,McGann LE,Acker JP

    更新日期:2014-06-01 00:00:00

  • Effect of chemical modifications on freeze denaturation of lactate dehydrogenase.

    abstract::Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(89)90046-1

    authors: Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

    更新日期:1989-04-01 00:00:00

  • Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide.

    abstract::Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use. However, one major obstacle to the manufacturing of clinical grade stem cells is the need for current good manufacturing practice...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2011.02.003

    authors: Seo JM,Sohn MY,Suh JS,Atala A,Yoo JJ,Shon YH

    更新日期:2011-06-01 00:00:00

  • In vitro colony formation by cryopreserved primary human tumor cells.

    abstract::The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of n...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(84)90215-3

    authors: Allen ED,Gau TC,Natale RB

    更新日期:1984-04-01 00:00:00

  • Fractures in cryopreserved arteries.

    abstract::The common carotid artery of the rabbit, a typical small elastic artery, can be cryopreserved using dimethyl sulfoxide, slow cooling, storage at less than -160 degrees C, and rapid warming. This technique provides satisfactory preservation of muscle and endothelial cells, but in about 75% of cases, gross circumferenti...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1994.1061

    authors: Hunt CJ,Song YC,Bateson EA,Pegg DE

    更新日期:1994-10-01 00:00:00

  • Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen.

    abstract::This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2017.03.002

    authors: Seifi-Jamadi A,Ahmad E,Ansari M,Kohram H

    更新日期:2017-04-01 00:00:00

  • The effects of preservation procedures on amniotic membrane's ability to serve as a substrate for cultivation of endothelial cells.

    abstract::Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibili...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2011.08.003

    authors: Niknejad H,Deihim T,Solati-Hashjin M,Peirovi H

    更新日期:2011-12-01 00:00:00

  • An amino acidic adjuvant to augment cryoinjury of MCF-7 breast cancer cells.

    abstract::One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2008.05.007

    authors: Wang CL,Teo KY,Han B

    更新日期:2008-08-01 00:00:00

  • Percutaneous comprehensive cryoablation for metastatic esophageal cancer after failure of radical surgery.

    abstract::Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:

    authors: Jiongyuan X,Lizhi N,Feng M,Shupeng L,Yin L,Mengtian L,Jianying Z,Fei Y,Jibing C,Jialiang L,Kecheng X

    更新日期:2013-12-01 00:00:00

  • Development of a freeze-drying protocol for the long-term storage of S9-fraction at ambient temperatures.

    abstract::The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2008.11.007

    authors: Buchinger S,Campen E,Helmers E,Morosow V,Krefft M,Reifferscheid G

    更新日期:2009-04-01 00:00:00

  • Effect of cryopreservation on apple genetic resources at morphological, chromosomal, and molecular levels.

    abstract::Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.2001.2339

    authors: Hao YJ,Liu QL,Deng XX

    更新日期:2001-08-01 00:00:00

  • Protective effects of osmolytes in cryopreserving adherent neuroblastoma (Neuro-2a) cells.

    abstract::A simple method to cryopreserve adherent monolayers of neuronal cells is currently not available, but the development of this technique could facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. However, complex tissues of some exceptional animals survive ...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2015.08.015

    authors: Bailey TL,Wang M,Solocinski J,Nathan BP,Chakraborty N,Menze MA

    更新日期:2015-12-01 00:00:00

  • Cellular and molecular consequences of reduced cell water content.

    abstract::Cell volumes, viability, ultrastructure, and metabolism have been studied in mouse L cells at reduced water contents. Dehydration was achieved by addition of sorbitol to the incubation medium at concentrations of 0.15, 0.3, and 0.6 m. In 0.3 m sorbitol, cell volume was reduced to about 35% of the initial control level...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(83)90048-2

    authors: Mansell JL,Clegg JS

    更新日期:1983-10-01 00:00:00

  • Viral contamination of embryos cryopreserved in liquid nitrogen.

    abstract::Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods ...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1999.2227

    authors: Bielanski A,Nadin-Davis S,Sapp T,Lutze-Wallace C

    更新日期:2000-03-01 00:00:00

  • Cytoprotective effect of glycine in cold stored canine renal tubules.

    abstract::Reperfusion injury has been suggested to cause delayed graft function in renal transplantation. Methods to reduce reperfusion injury could lead to improved clinical renal transplantation. Glycine has been shown to suppress reperfusion injury in rabbit renal tubules and rat hepatocytes. In this study we have determined...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1993.1022

    authors: Saunder A,Ametani MS,Belzer FO,Southard JH

    更新日期:1993-06-01 00:00:00

  • Isolation of ice-nucleating active bacteria from the freeze-tolerant frog, Rana sylvatica.

    abstract::Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1995.1036

    authors: Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

    更新日期:1995-08-01 00:00:00

  • Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing.

    abstract::Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/s0011-2240(02)00181-5

    authors: Hunt CJ,Armitage SE,Pegg DE

    更新日期:2003-02-01 00:00:00

  • Effects of different cryoprotectants on the viability and biological characteristics of porcine preadipocyte.

    abstract::Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2006.06.007

    authors: Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

    更新日期:2006-10-01 00:00:00

  • Cryopreservation of Radopholus similis, a tropical plant-parasitic nematode.

    abstract::For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2007.06.006

    authors: Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

    更新日期:2007-10-01 00:00:00

  • Effect of sugars in the preservation solution on liver storage in rats.

    abstract::We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(91)90051-o

    authors: Kobayashi T,Sumimoto R,Shimada H,Kamada N,Nakagawara G

    更新日期:1991-10-01 00:00:00

  • Functional preservation of the mammalian kidney. IV. Functional effects of perfusion with dimethyl sulfoxide (DMSO) at normothermia.

    abstract::Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(76)90101-2

    authors: Karow AM Jr,Jeske AH

    更新日期:1976-08-01 00:00:00

  • The effects of different concentrations of glycerol and dimethylsulfoxide on the metabolic activities of kidney slices.

    abstract::Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure t...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(83)90059-7

    authors: Penninckx F,Cheng N,Kerremans R,Van Damme B,De Loecker W

    更新日期:1983-02-01 00:00:00

  • Improved low-CPA vitrification of mouse oocytes using quartz microcapillary.

    abstract::Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2015.04.003

    authors: Choi JK,Huang H,He X

    更新日期:2015-06-01 00:00:00