Abstract:
:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facilitate transfer of liver tissues between distant centers for cell isolation studies.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Caruana M,Battle T,Fuller B,Davidson Bdoi
10.1006/cryo.1999.2155subject
Has Abstractpub_date
1999-03-01 00:00:00pages
165-8issue
2eissn
0011-2240issn
1090-2392pii
S0011-2240(99)92155-7journal_volume
38pub_type
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