Improved low-CPA vitrification of mouse oocytes using quartz microcapillary.

abstract：:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...

journal_title：Cryobiology

authors： Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JC

更新日期：2001-02-01 00:00:00

abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

journal_title：Cryobiology

authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

更新日期：2007-10-01 00:00:00

abstract：:Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...

journal_title：Cryobiology

authors： Shaw JM,Trounson AO

更新日期：1989-10-01 00:00:00

abstract：:The effects of rapid cooling of semen (cold shock) from 30 degrees C to various temperatures above 0 degrees C on survival of ram spermatozoa suspended in diluents with or without egg yolk were assessed before and after freezing. Rapid cooling of extended semen from 30 to 15 degrees C had little or no effect on sperma...

journal_title：Cryobiology

authors： Fiser PS,Fairfull RW

更新日期：1986-12-01 00:00:00

abstract：:The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...

journal_title：Cryobiology

authors： Song WJ,Jiji LM

更新日期：1988-04-01 00:00:00

abstract：:The Arrhenius plots of inactivation (killing) rates of five mammalian cell lines, V-79 Chinese hamster lung, mouse L-929, mouse neuroblastoma, human 18LU, and human erythrocytes, exposed to hypothermia contain a break somewhere between 5 and 10 degrees C caused by a change from positive to negative slope, which corres...

journal_title：Cryobiology

authors： Kruuv J,Glofcheski DJ,Lepock JR

更新日期：1995-04-01 00:00:00

abstract：:The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...

journal_title：Cryobiology

authors： Yoshimoto T,Nakamura S,Yamauchi S,Muto N,Nakada T,Ashizawa K,Tatemoto H

更新日期：2008-08-01 00:00:00

abstract：:Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the cult...

journal_title：Cryobiology

authors： Del Pino A,Ligero G,López MB,Navarro H,Carrillo JA,Pantoll SC,Díaz de la Guardia R

更新日期：2015-02-01 00:00:00

abstract：:Despite some 26 published reports addressing oyster sperm cryopreservation, systematic factor optimization is lacking, and sperm cryopreservation has not yet found application in aquaculture on a commercial scale. In this study, the effects of cooling rate, single or combined cryoprotectants at various concentrations,...

journal_title：Cryobiology

authors： Dong Q,Huang C,Eudeline B,Tiersch TR

更新日期：2005-10-01 00:00:00

abstract：:In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...

journal_title：Cryobiology

authors： Tymczyszyn EE,Gerbino E,Illanes A,Gómez-Zavaglia A

更新日期：2011-04-01 00:00:00

abstract：:There is increasing interest in the possibility of treating diseased or damaged areas of synovial joint surfaces by grafts of healthy allogeneic cartilage. Such grafts could be obtained from cadaver tissue donors or in the future they might be manufactured by 'tissue engineering' methods. Cartilage is an avascular tis...

journal_title：Cryobiology

authors： Pegg DE,Wusteman MC,Wang L

更新日期：2006-06-01 00:00:00

abstract：:The utility of microalgal biomass and bioproducts depends on long-term maintenance of certain physiological or biochemical features of the species. While unique characteristics may not be durably maintained with general subculture, cryopreservation methods better prevent alterations from desired characteristics. Post-...

journal_title：Cryobiology

authors： Zheng L,Lu Z,Zhang Q,Li T,Song L

更新日期：2018-04-01 00:00:00

abstract：:Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0...

journal_title：Cryobiology

authors： Correia LFL,Espírito-Santo CG,Braga RF,Carvalho-de-Paula CJ,da Silva AA,Brandão FZ,Freitas VJF,Ungerfeld R,Souza-Fabjan JMG

更新日期：2020-11-10 00:00:00

abstract：:As known for different metabolic functions, α-lipoic acid (ALA) has been tested for spermatozoa preservation of animals as well as of human, but not for fish spermatozoa. The present study determined the effects of ALA on short and long-term (cryopreservation) preservation of common carp (Cyprinus carpio) spermatozoa,...

journal_title：Cryobiology

authors： İnanan BE,Kanyılmaz M

更新日期：2020-06-01 00:00:00

abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

journal_title：Cryobiology

authors： Biggar KK,Dubuc A,Storey K

更新日期：2009-12-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...

journal_title：Cryobiology

authors： Yu SO,Brown A,Middleton AJ,Tomczak MM,Walker VK,Davies PL

更新日期：2010-12-01 00:00:00

abstract：:The Zebrafish has gained increased popularity as an aquatic model species in various research fields, and its widespread use has led to numerous mutant strains and transgenic lines. This creates the need to store these important genetic materials as frozen gametes. Sperm cryopreservation in zebrafish has been shown to...

journal_title：Cryobiology

authors： Bai C,Wang X,Lu G,Wei L,Liu K,Gao H,Huang C,Dong Q

更新日期：2013-10-01 00:00:00

abstract：:In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...

journal_title：Cryobiology

authors： Pomorski L,Bartos M,Matejkowska M,Amsolik M,Kuzdak K

更新日期：1999-11-01 00:00:00

abstract：:Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...

journal_title：Cryobiology

authors： Batista Lobo S,Denyer M,Gopalan RC,Javid FA

更新日期：2008-10-01 00:00:00

abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.cryobiol.2009.05.007. The duplicate article has therefore been withdrawn. ...

journal_title：Cryobiology

authors： Wowk B

更新日期：2009-06-06 00:00:00

abstract：:Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influenc...

journal_title：Cryobiology

authors： Orjuela-Palacio JM,Graiver N,Santos MV,Zaritzky NE

更新日期：2019-08-01 00:00:00

abstract：:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...

journal_title：Cryobiology

authors： Bartrons M,Ortega E,Obach M,Calvo MN,Navarro-Sabaté A,Bartrons R

更新日期：2004-10-01 00:00:00

abstract：:Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...

journal_title：Cryobiology

authors： Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

更新日期：2019-10-01 00:00:00

abstract：:To enrich bovine gonocytes from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined. Using Minimum essential medium (MEM) + 10% dimethyl sulfoxide (Me2SO) as the basic medium, calf testicular tissues were cryopreserved in media co...

journal_title：Cryobiology

authors： Jiang Y,Zhu WQ,Zhu XC,Cai NN,Yang R,Cai H,Zhang XM

更新日期：2020-02-01 00:00:00

abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

journal_title：Cryobiology

authors： Wusteman M,Boylan S,Pegg DE

更新日期：1996-08-01 00:00:00

abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...

journal_title：Cryobiology

authors： Cheng LY,Sun TC,Liu XC,Yu H,Zhou SJ,Tian L,Yang SH,Liu BX

更新日期：2020-12-03 00:00:00

abstract：:Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...

journal_title：Cryobiology

authors： Hunt CJ,Armitage SE,Pegg DE

更新日期：2003-02-01 00:00:00

abstract：:We make some crucial remarks about the recent review by Buitink and Leprince (2004) [Cryobiology 48 (2004) 215-228] considering their false definition about the glass transition temperature based on the value of (shear) viscosity: 1014 Pa⋅s. The correct value is 1012 Pa⋅s as evidenced from http://goldbook.iupac.org/ht...

journal_title：Cryobiology

authors： Kwang-Hua CW

更新日期：2018-08-01 00:00:00

abstract：:Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...

journal_title：Cryobiology

authors： Baudot A,Cacela C,Duarte ML,Fausto R

更新日期：2002-04-01 00:00:00