Abstract：
:The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice-nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleating methods may suffer from cumbersome manual operations, safety concerns of external fields, and biocompatibility and recovery issues of INA particles, especially when used in living systems. Given the automatic ice-seeding nature of INAs, a promising solution to overcome some of the above limitations is to engineer a biocomposite that accommodates the INA particles but minimizes their interactions with biologics, as well as enabling the recovery of used particles. In this study, freeze-dried Pseudomonas syringae, a model ice-nucleating agent, was encapsulated into microliter-sized alginate beads. We evaluated the performance of the bacterial hydrogel beads to initiate ice nucleation in water and aqueous glycerol solution by investigating factors including the size and number of the beads and the local concentration of INA particles. In the aqueous sample of a fixed volume, the total mass of the INA particles (m) was found to be the governing parameter that is solely responsible for determining the ice nucleation performance of the bacterial hydrogel beads. The freezing temperature has a strong positive linear correlation with log10m. The findings in this study provide an effective, predictable approach to control ice nucleation, which can improve the outcome and standardization of many ice-assisted process technologies.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Weng L,Tessier SN,Swei A,Stott SL,Toner Mdoi
10.1016/j.cryobiol.2017.03.006subject
Has Abstractpub_date
2017-04-01 00:00:00pages
1-6eissn
0011-2240issn
1090-2392pii
S0011-2240(17)30029-9journal_volume
75pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1994.1023
更新日期：1994-04-01 00:00:00
abstract：:A total of 498 porcine embryos at various stages of development collected from superovulated gilts was used to investigate cryopreservation. First, blastocysts (BL), expanded blastocysts (ExB), and hatched blastocysts (HB) were used to determine the effect of exposure to concentrated solutions of ethylene glycol as cr...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1997.2056
更新日期：1998-02-01 00:00:00
abstract：:Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2146
更新日期：1999-05-01 00:00:00
abstract：:This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.01.002
更新日期：2008-04-01 00:00:00
abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(92)90022-t
更新日期：1992-04-01 00:00:00
abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.07.006
更新日期：2015-10-01 00:00:00
abstract：:We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.05.009
更新日期：2017-08-01 00:00:00
abstract：:Vitrification is a promising alternative to tissue preservation, in which the tissue is permeated with cryoprotective agents (CPAs) in order to circumvent the hazardous effects associated with ice formation. In this study, we evaluate the effect of vitreous cryopreservation of rabbit trachea, by comparing vitrificatio...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.12.009
更新日期：2009-04-01 00:00:00
abstract：:Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2016.10.006
更新日期：2016-12-01 00:00:00
abstract：:The ability to desiccate mammalian cells while maintaining a high degree of viability would have implications for many areas of biological science, including tissue engineering. Previously, we reported that introduction of the genes for trehalose biosynthesis allowed human cells in culture to be reversibly desiccated ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.2001.2324
更新日期：2001-05-01 00:00:00
abstract：:We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/s0011-2240(02)00123-2
更新日期：2002-10-01 00:00:00
abstract：:Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw function of sperm from fi...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.04.003
更新日期：2014-06-01 00:00:00
abstract：:Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.11.001
更新日期：2020-11-10 00:00:00
abstract：:The effect of cryopreservation on ciliary activity of human nasal respiratory epithelium was evaluated. Samples were cryopreserved in a solution containing nutrient medium, 10% fetal calf serum, and two different concentrations (10 or 20%) of dimethyl sulfoxide and stored in liquid nitrogen at -196 degrees C for 2 wee...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90056-4
更新日期：1989-08-01 00:00:00
abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2006.03.011
更新日期：2006-08-01 00:00:00
abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2085
更新日期：1998-06-01 00:00:00
abstract：:Our objective was to study the effect of the concentration of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO) during vitrification on the development of porcine blastocysts. Vitrification was performed with 0.4 M sucrose and either a Me2SO and EG mixture (15%, 16% and 17% v/v of each) or EG alone (40% v/v), using ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.02.005
更新日期：2008-06-01 00:00:00
abstract：:The development of efficient and robust methods for the cryopreservation of human embryonic stem cells (hESCs) is important for the production of master and working cell banks for future clinical applications. Such methods must meet requirements of good manufacturing practice (GMP) and maintain genetic stability of th...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2010.03.007
更新日期：2010-06-01 00:00:00
abstract：:Toxoplasma gondii is a protozoan parasite that infects birds and mammals, including humans. T. gondii T-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure for T. gondii T-263 bradyzoites has been developed to meet the require...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1996.0033
更新日期：1996-06-01 00:00:00
abstract：:Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.01.002
更新日期：2017-04-01 00:00:00
abstract：:We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2006.05.005
更新日期：2006-10-01 00:00:00
abstract：:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.07.004
更新日期：2008-10-01 00:00:00
abstract：:The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.04.002
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.06.006
更新日期：2005-10-01 00:00:00
abstract：:The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown is particularly sensitive to cold and readily damaged by exposure to low temperatures, the da...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1996.0055
更新日期：1996-10-01 00:00:00
abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.2001.2306
更新日期：2001-03-01 00:00:00
abstract：PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2021.01.005
更新日期：2021-01-02 00:00:00
abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2106
更新日期：1998-09-01 00:00:00
abstract：:The cause of slow freezing injury and the basis of the protection by solutes like glycerol are subjects of debate. During slow freezing, cells are sequestered in unfrozen channels between ice crystals that grow by removing pure water from the channels. As a consequence, the solute concentration in the channels rises a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90030-8
更新日期：1989-02-01 00:00:00
abstract：:A simple method to cryopreserve adherent monolayers of neuronal cells is currently not available, but the development of this technique could facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. However, complex tissues of some exceptional animals survive ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.08.015
更新日期：2015-12-01 00:00:00
