The effects of preservation procedures on amniotic membrane's ability to serve as a substrate for cultivation of endothelial cells.

abstract：:The development of drugs in cardiovascular research involves in vitro studies that are generally performed on freshly isolated blood vessels. Naturally, data obtained from human tissues would be the most accurate and predictable for human pharmacology. However, the main problems are that the supply of fresh human mate...

journal_title：Cryobiology

authors： Müller-Schweinitzer E

更新日期：1994-02-01 00:00:00

abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

journal_title：Cryobiology

authors： Wusteman M,Boylan S,Pegg DE

更新日期：1996-08-01 00:00:00

abstract：:Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects ...

journal_title：Cryobiology

authors： Osipova VP,Berberova NT,Gazzaeva RA,Kudryavtsev KV

更新日期：2016-04-01 00:00:00

abstract：:Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two ho...

journal_title：Cryobiology

authors： Nath J,Smith TB,Patel K,Ebbs SR,Hollis A,Tennant DA,Ludwig C,Ready AR

更新日期：2017-02-01 00:00:00

abstract：:The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...

journal_title：Cryobiology

authors： Acker JP,McGann LE

更新日期：2000-02-01 00:00:00

abstract：:This brief paper introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist. ...

journal_title：Cryobiology

authors： Elliott JA

更新日期：2010-02-01 00:00:00

abstract：:High concentrations of membrane permeable cryoprotectants are necessary to protect human polymorphonuclear leukocytes from osmotic stress injury during freezing, but there are reports that some cryoprotectants are chemically toxic. Cells were exposed to various concentrations of glycerol, dimethyl sulfoxide, or ethyle...

journal_title：Cryobiology

authors： Takahashi T,Bross JB,Shaber RE,Williams RJ

更新日期：1985-08-01 00:00:00

abstract：:Bone spavin, splint, and fractured splint bone injuries have been treated with varying methodologies at Wheatley Hall Farm Equine Clinic. Cryosurgery is the most successful. With cryosurgery the small, pain-producing afferent C fibers are destroyed, and painful neuromas do not return. Injured sites were cryosurgically...

journal_title：Cryobiology

authors： McKibbin LS,Paraschak DM

更新日期：1985-10-01 00:00:00

abstract：:This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...

journal_title：Cryobiology

authors： Seifi-Jamadi A,Ahmad E,Ansari M,Kohram H

更新日期：2017-04-01 00:00:00

abstract：PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...

journal_title：Cryobiology

authors： Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

更新日期：2017-04-01 00:00:00

abstract：:Two parameters fundamental to cell cryobiology are the water permeability (hydraulic conductivity), Lp, and its activation energy, EA. The Lp can be calculated from two experimental determinations: the critical osmolality, Osmcrit, at which 50% of the cells lyse, and the time, tcrit, to 50% lysis in a highly hyposmoti...

journal_title：Cryobiology

authors： Noiles EE,Thompson KA,Storey BT

更新日期：1997-08-01 00:00:00

abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...

journal_title：Cryobiology

authors： Marco-Jiménez F,Garzón DL,Peñaranda DS,Pérez L,Viudes-de-Castro MP,Vicente JS,Jover M,Asturiano JF

更新日期：2006-08-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:The Zebrafish has gained increased popularity as an aquatic model species in various research fields, and its widespread use has led to numerous mutant strains and transgenic lines. This creates the need to store these important genetic materials as frozen gametes. Sperm cryopreservation in zebrafish has been shown to...

journal_title：Cryobiology

authors： Bai C,Wang X,Lu G,Wei L,Liu K,Gao H,Huang C,Dong Q

更新日期：2013-10-01 00:00:00

abstract：:Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...

journal_title：Cryobiology

authors： Browne RK,Mahony M,Clulow J

更新日期：2002-06-01 00:00:00

abstract：:Human monocytes purified from peripheral blood by counterflow centrifugal elutriation were cryopreserved in a vitreous state at 1 atm pressure. The vitrification solution was Hanks' balanced salt solution (HBSS) containing (w/v) 20.5% Me2SO, 15.5% acetamide, 10% propylene glycol, and 6% polyethylene glycol. Fifteen mi...

journal_title：Cryobiology

authors： Takahashi T,Hirsh A,Erbe EF,Bross JB,Steere RL,Williams RJ

更新日期：1986-04-01 00:00:00

abstract：:We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...

journal_title：Cryobiology

authors： Kobayashi T,Sumimoto R,Shimada H,Kamada N,Nakagawara G

更新日期：1991-10-01 00:00:00

abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...

journal_title：Cryobiology

authors： Robles V,Cabrita E,Real M,Alvarez R,Herráez MP

更新日期：2003-08-01 00:00:00

abstract：:Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechano...

journal_title：Cryobiology

authors： Traversari G,Cincotti A

更新日期：2020-11-27 00:00:00

abstract：:The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was de...

journal_title：Cryobiology

authors： Šćekić I,Marinović Z,Lujić J,Müller T,Kitanović N,Urbányi B,Horváth Á

更新日期：2020-08-01 00:00:00

abstract：:We have shown previously that the survival of human red blood cells during slow freezing at 2% hematocrit is dependent more on the magnitude of the unfrozen fraction than on the salt concentration in that unfrozen fraction. In parallel, first Nei and more recently Pegg and colleagues have shown that survival is affect...

journal_title：Cryobiology

authors： Mazur P,Cole KW

更新日期：1985-12-01 00:00:00

abstract：:The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...

journal_title：Cryobiology

authors： Buchinger S,Campen E,Helmers E,Morosow V,Krefft M,Reifferscheid G

更新日期：2009-04-01 00:00:00

abstract：:This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...

journal_title：Cryobiology

authors： Zhang T,Rawson DM,Tosti L,Carnevali O

更新日期：2008-04-01 00:00:00

abstract：:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...

journal_title：Cryobiology

authors： Bartrons M,Ortega E,Obach M,Calvo MN,Navarro-Sabaté A,Bartrons R

更新日期：2004-10-01 00:00:00

abstract：:The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice-nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleatin...

journal_title：Cryobiology

authors： Weng L,Tessier SN,Swei A,Stott SL,Toner M

更新日期：2017-04-01 00:00:00

abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...

journal_title：Cryobiology

authors： Edashige K,Asano A,An TZ,Kasai M

更新日期：1999-06-01 00:00:00

abstract：:Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilizati...

journal_title：Cryobiology

authors： Moawad AR,Fisher P,Zhu J,Choi I,Polgar Z,Dinnyes A,Campbell KH

更新日期：2012-10-01 00:00:00

abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

journal_title：Cryobiology

authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

更新日期：1997-11-01 00:00:00

abstract：:The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...

journal_title：Cryobiology

authors： Song WJ,Jiji LM

更新日期：1988-04-01 00:00:00

abstract：:Tambaqui (Colossoma macropomum) is a freshwater fish of great importance to aquaculture in several South American countries. Recent studies have developed a protocol for semen cryopreservation in 0.25 and 0.5 mL straws; however, this technique has limitations for fingerling production at a large scale due to the high ...

journal_title：Cryobiology

authors： Maria AN,Carvalho AC,Araújo RV,Santos JP,Carneiro PC,Azevedo HC

更新日期：2015-04-01 00:00:00