Development of a freeze-drying protocol for the long-term storage of S9-fraction at ambient temperatures.

abstract：:The ability to routinely cryopreserve micro-algal species reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift. Cryopreservation is also a useful adjunct in aquaculture hatcheries for strains of micro-a...

journal_title：Cryobiology

authors： Rhodes L,Smith J,Tervit R,Roberts R,Adamson J,Adams S,Decker M

更新日期：2006-02-01 00:00:00

abstract：:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. ...

journal_title：Cryobiology

authors： Zeng C,Peng W,Ding L,He L,Zhang Y,Fang D,Tang K

更新日期：2014-08-01 00:00:00

abstract：:The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...

journal_title：Cryobiology

authors： Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

更新日期：2001-11-01 00:00:00

abstract：:The distribution of cryoprotectant (10% glycerol) and ice along the frozen plastic straw (the most useful container for freezing mammalian semen, oocytes and embryos) was studied by Raman scattering technique. Raman spectroscopy being a contactless, non-invasive tool was applied for the straws filled with the cryoprot...

journal_title：Cryobiology

authors： Karpegina YA,Okotrub KA,Brusentsev EY,Amstislavsky SY,Surovtsev NV

更新日期：2016-04-01 00:00:00

abstract：:The crystallization of ice from aqueous solutions of glycerol and dimethyl sulfoxide (Me2SO) has been studied using differential scanning calorimetry. In particular, the ice crystallization behavior of glycerol and Me2SO solutions containing approximately the same mole percent solute concentration (i.e., approximately...

journal_title：Cryobiology

authors： Hey JM,Macfarlane DR

更新日期：1996-04-01 00:00:00

abstract：:Desiccation preservation holds promise as a simplified alternative to cryopreservation for the long term storage of cells. We report a study on the protective effects of intracellular and extracellular sugars during bovine sperm desiccation and the supplemental effects of the addition of an antioxidant (catalase) or a...

journal_title：Cryobiology

authors： Sitaula R,Elmoazzen H,Toner M,Bhowmick S

更新日期：2009-06-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...

journal_title：Cryobiology

authors： Cheng LY,Sun TC,Liu XC,Yu H,Zhou SJ,Tian L,Yang SH,Liu BX

更新日期：2020-12-03 00:00:00

abstract：:Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20...

journal_title：Cryobiology

authors： McClean R,Zee YP,Holt WV,Johnston SD

更新日期：2008-12-01 00:00:00

abstract：:Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influenc...

journal_title：Cryobiology

authors： Orjuela-Palacio JM,Graiver N,Santos MV,Zaritzky NE

更新日期：2019-08-01 00:00:00

abstract：:One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study w...

journal_title：Cryobiology

authors： Kanias T,Acker JP

更新日期：2009-04-01 00:00:00

abstract：:1,3-Butanediol and 2,3-butanediol have been used in buffered solutions with 20, 30, or 35% (w/w) alcohol to cool erythrocytes to -196 degrees C at different cooling rates between 1 to 3500 degrees C/min, followed by slow or rapid rewarming. 1,3-butanediol shows the same shapes of red blood cell survival curves as 1,2-...

journal_title：Cryobiology

authors： Mehl P,Boutron P

更新日期：1988-02-01 00:00:00

abstract：:We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....

journal_title：Cryobiology

authors： Zeron Y,Tomczak M,Crowe J,Arav A

更新日期：2002-10-01 00:00:00

abstract：:Appropriate timing of cold deacclimation is an important component of winter survival of perennial plants, such as trees, in temperate and boreal zones. Recently, concerns about predicted global climate change disturbing deacclimation timing have been increasing. The relationship between ambient temperatures and the m...

journal_title：Cryobiology

authors： Takeuchi M,Kasuga J

更新日期：2018-02-01 00:00:00

abstract：:Organ preservation solutions have been designed to protect grafts against the injury inflicted by cold ischemia. However, toxicity of University of Wisconsin (UW) solution during rewarming has been reported. Therefore, we here assessed the toxicity of UW, histidine-tryptophan-ketoglutarate (HTK), Euro-Collins, histidi...

journal_title：Cryobiology

authors： Rauen U,de Groot H

更新日期：2008-02-01 00:00:00

abstract：:We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...

journal_title：Cryobiology

authors： Bourne WM,Nelson LR,Hodge DO

更新日期：1999-08-01 00:00:00

abstract：:The present study aimed to examine the behavior of ram spermatozoa subjected to a vitrification process in free-egg yolk diluents in relation with conventional diluents and cryopreservation protocol used in this species. Previously it was investigated the toxicity of cryoprotectants, sucrose and glycerol, based on dif...

journal_title：Cryobiology

authors： Jiménez-Rabadán P,García-Álvarez O,Vidal A,Maroto-Morales A,Iniesta-Cuerda M,Ramón M,del Olmo E,Fernández-Santos R,Garde JJ,Soler AJ

更新日期：2015-08-01 00:00:00

abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

journal_title：Cryobiology

authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

更新日期：2007-10-01 00:00:00

abstract：:Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that ...

journal_title：Cryobiology

authors： Jin B,Kleinhans FW,Mazur P

更新日期：2014-06-01 00:00:00

abstract：:In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...

journal_title：Cryobiology

authors： Pomorski L,Bartos M,Matejkowska M,Amsolik M,Kuzdak K

更新日期：1999-11-01 00:00:00

abstract：:One thousand cryohemorrhoidectomies were performed in the past 7 years (1973-1979). Ninety percent of the patients were satisfied with the technique; approximately one-third of the patients experienced severe to moderate pain and 11% had recurrences or related problems. The majority of patients with postoperative pain...

journal_title：Cryobiology

authors： Oh C

更新日期：1982-06-01 00:00:00

abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

journal_title：Cryobiology

authors： Wusteman M,Boylan S,Pegg DE

更新日期：1996-08-01 00:00:00

abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.cryobiol.2009.05.007. The duplicate article has therefore been withdrawn. ...

journal_title：Cryobiology

authors： Wowk B

更新日期：2009-06-06 00:00:00

abstract：:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facil...

journal_title：Cryobiology

authors： Caruana M,Battle T,Fuller B,Davidson B

更新日期：1999-03-01 00:00:00

abstract：:Percutaneous ablation is the currently preferred locoregional therapy for non-resectable hepatocellular cancer (HCC). Cryoablation is an attractive option because it forms an ice ball viewable by many imaging methods. This study assessed the therapeutic effect of comprehensive cryoablation (of intra- and extrahepatic ...

journal_title：Cryobiology

authors： Mu F,Niu L,Li H,Liao M,Li L,Liu C,Chen J,Li J,Zuo J,Xu K

更新日期：2013-02-01 00:00:00

abstract：:Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...

journal_title：Cryobiology

authors： Warner RM,Ampo E,Nelson D,Benson JD,Eroglu A,Higgins AZ

更新日期：2020-11-04 00:00:00

abstract：:Among the various types of stem cells, induced pluripotent stem cells (iPSCs) have gained much attention due to their pluripotent nature. iPSCs help us to understand the processes that regulate pluripotency and specialization. However, in order to use them in various applications in regenerative medicine, their effici...

journal_title：Cryobiology

authors： Fernandes S,Khan N,Kale V,Limaye L

更新日期：2019-10-01 00:00:00

abstract：:Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...

journal_title：Cryobiology

authors： Batista Lobo S,Denyer M,Gopalan RC,Javid FA

更新日期：2008-10-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:Oxidative stress is one of the major issues associated with cryopreservation because it causes a marked reduction in the post-thaw quality of semen. This study investigated the ability of kinetin to preserve the structural and functional integrity of dog sperm during cryopreservation. Pooled ejaculates were divided in...

journal_title：Cryobiology

authors： Qamar AY,Fang X,Bang S,Kim MJ,Cho J

更新日期：2020-08-01 00:00:00