The effect of liposomes on thermotropic membrane phase transitions of bovine spermatozoa and oocytes: implications for reducing chilling sensitivity.

abstract：:Cold preservation and reperfusion of liver during transplantation are necessary steps in the procedure but which are also associated with damage to the organ. One aspect of this damage is thought to concern up-regulation of inflammatory markers, such as the adhesion molecule intercellular adhesion molecule 1 (ICAM-1) ...

journal_title：Cryobiology

authors： El-Wahsh M,Fuller B,Davidson B,Rolles K

更新日期：2003-10-01 00:00:00

abstract：:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...

journal_title：Cryobiology

authors： Bartrons M,Ortega E,Obach M,Calvo MN,Navarro-Sabaté A,Bartrons R

更新日期：2004-10-01 00:00:00

abstract：:In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...

journal_title：Cryobiology

authors： Tymczyszyn EE,Gerbino E,Illanes A,Gómez-Zavaglia A

更新日期：2011-04-01 00:00:00

abstract：:A previous study demonstrated that disaccharides, antioxidants, and caspase inhibitors can be used in freezing solutions to reduce the concentration of Me2SO from the current standard of 10% (v/v) to 5% (v/v) or 2.5% and to eliminate fetal bovine serum (FBS) for the cryopreservation of human amniotic fluid-derived ste...

journal_title：Cryobiology

authors： Cho HJ,Lee SH,Yoo JJ,Shon YH

更新日期：2014-04-01 00:00:00

abstract：:Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...

journal_title：Cryobiology

authors： Anil S,Rawson D,Zhang T

更新日期：2018-08-01 00:00:00

abstract：:The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was de...

journal_title：Cryobiology

authors： Šćekić I,Marinović Z,Lujić J,Müller T,Kitanović N,Urbányi B,Horváth Á

更新日期：2020-08-01 00:00:00

abstract：:To enrich bovine gonocytes from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined. Using Minimum essential medium (MEM) + 10% dimethyl sulfoxide (Me2SO) as the basic medium, calf testicular tissues were cryopreserved in media co...

journal_title：Cryobiology

authors： Jiang Y,Zhu WQ,Zhu XC,Cai NN,Yang R,Cai H,Zhang XM

更新日期：2020-02-01 00:00:00

abstract：:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...

journal_title：Cryobiology

authors： Storey KB

更新日期：2004-04-01 00:00:00

abstract：PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...

journal_title：Cryobiology

authors： Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

更新日期：2017-04-01 00:00:00

abstract：:Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...

journal_title：Cryobiology

authors： Batista Lobo S,Denyer M,Gopalan RC,Javid FA

更新日期：2008-10-01 00:00:00

abstract：:In an experimental study using porcine corneas we investigated the influence of several variables of freeze-thaw trauma on survival of corneal endothelial cells after corneal cryopreservation employing chondroitin sulfate as a cryoprotectant. We examined the influence of: (1) the concentration of chondroitin sulfate i...

journal_title：Cryobiology

authors： Hagenah M,Böhnke M

更新日期：1993-08-01 00:00:00

abstract：:Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechano...

journal_title：Cryobiology

authors： Traversari G,Cincotti A

更新日期：2020-11-27 00:00:00

abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...

journal_title：Cryobiology

authors： Lewis JG,Learmonth RP,Watson K

更新日期：1994-04-01 00:00:00

abstract：:The effect of cryopreservation on human nasal mucosal biopsies was evaluated by determining the frequency and coordination of the ciliary beat. Samples were cryopreserved in a medium containing 80% Gey's balanced salt solution, 10% dimethyl sulfoxide, and 10% fetal calf serum. After thawing, the samples were put in a ...

journal_title：Cryobiology

authors： Wulffraat NM,Veerman AJ,Stamhuis IH

更新日期：1985-04-01 00:00:00

abstract：:We have shown previously that the survival of human red blood cells during slow freezing at 2% hematocrit is dependent more on the magnitude of the unfrozen fraction than on the salt concentration in that unfrozen fraction. In parallel, first Nei and more recently Pegg and colleagues have shown that survival is affect...

journal_title：Cryobiology

authors： Mazur P,Cole KW

更新日期：1985-12-01 00:00:00

abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...

journal_title：Cryobiology

authors： Robles V,Cabrita E,Real M,Alvarez R,Herráez MP

更新日期：2003-08-01 00:00:00

abstract：:The effect of low temperatures on the ultrastructure of the plasma membrane of bull and boar spermatozoa was investigated. Cold-induced changes in the organization of sperm plasma membrane components were demonstrated by the use of fast-freezing combined with freeze-fracture electron microscopy. This preparation techn...

journal_title：Cryobiology

authors： De Leeuw FE,Chen HC,Colenbrander B,Verkleij AJ

更新日期：1990-04-01 00:00:00

abstract：:The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of n...

journal_title：Cryobiology

authors： Allen ED,Gau TC,Natale RB

更新日期：1984-04-01 00:00:00

abstract：:We have studied the sensitivity to cryoprotecting agents of different embryos of the local sea urchin, Echinometra lucunter which is the species used for embryo-larval bioassays in Brazil. We have located significant differences between both species sensitivity to cryoprotecting agents; while for P. lividus propylene ...

journal_title：Cryobiology

authors： Ribeiro MB,Furley T,Spago FR,Paredes E

更新日期：2018-02-01 00:00:00

abstract：:The importance of the frozen phase in the formation of cryooscillations of trypsin activity has been shown in experiments conducted at -10 degrees C under frozen and supercooled conditions, respectively. A solution containing trypsin obtained by trypsinogen activation and 0.1 M MnCl2 was distributed in test tubes with...

journal_title：Cryobiology

authors： Vajda T

更新日期：1992-08-01 00:00:00

abstract：:The efficacy of attenuated strain of gdhA derivative Pasteurella multocida B:2 mutant as a live vaccine to control haemorrhagic septicaemia (HS) disease in cattle and buffaloes has been demonstrated. In order to use P. multocida B:2 mutant as a commercial product, it is essential to optimise its formulation for high v...

journal_title：Cryobiology

authors： Oslan SNH,Halim M,Ramle NA,Saad MZ,Tan JS,Kapri MR,Ariff AB

更新日期：2017-12-01 00:00:00

abstract：:Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...

journal_title：Cryobiology

authors： Armitage WJ,Juss BK

更新日期：2003-04-01 00:00:00

abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

journal_title：Cryobiology

authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

更新日期：1997-11-01 00:00:00

abstract：:The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ros...

journal_title：Cryobiology

authors： Orekhova A,Barták M,Hájek J

更新日期：2018-12-01 00:00:00

abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...

journal_title：Cryobiology

authors： Lautner LJ,Freed DH,Nagendran J,Acker JP

更新日期：2020-06-01 00:00:00

abstract：:A 2,3-butanediol containing 96.7% (w/w) racemic mixture of the levo and dextro isomers and only 3.1% (w/w) of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 degrees C at rates between 2 and 3500 degrees C/min, followed by slo...

journal_title：Cryobiology

authors： Boutron P

更新日期：1992-06-01 00:00:00

abstract：:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facil...

journal_title：Cryobiology

authors： Caruana M,Battle T,Fuller B,Davidson B

更新日期：1999-03-01 00:00:00

abstract：:Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...

journal_title：Cryobiology

authors： Bielanski A

更新日期：2005-04-01 00:00:00

abstract：:Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure t...

journal_title：Cryobiology

authors： Penninckx F,Cheng N,Kerremans R,Van Damme B,De Loecker W

更新日期：1983-02-01 00:00:00