First steps towards Echinometra lucunter embryo cryopreservation.

abstract：:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...

journal_title：Cryobiology

authors： Bartrons M,Ortega E,Obach M,Calvo MN,Navarro-Sabaté A,Bartrons R

更新日期：2004-10-01 00:00:00

abstract：:We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...

journal_title：Cryobiology

authors： Bourne WM,Nelson LR,Hodge DO

更新日期：1999-08-01 00:00:00

abstract：:Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20...

journal_title：Cryobiology

authors： McClean R,Zee YP,Holt WV,Johnston SD

更新日期：2008-12-01 00:00:00

abstract：:In order to evaluate the many variables that can affect cryopreservation success, a simple, highly reproducible model system is required. We have evaluated the use of tetrazolium reductase activity as a prognostic indicator of skin viability in an inbred murine model. Two inbred hairless mouse strains were characteriz...

journal_title：Cryobiology

authors： Kearney JN,Wheldon LA,Gowland G

更新日期：1990-02-01 00:00:00

abstract：:Hepatic cryotherapy is increasingly used in the treatment of patients with multiple hepatic metastases, particularly from colorectal cancer. The Cryotech LCS 2000 system, with insulated shaft-circulated liquid nitrogen probes, was designed for this purpose and was evaluated on the bench and in an animal model. The 9-m...

journal_title：Cryobiology

authors： Dilley AV,Dy DY,Warlters A,Copeland S,Gillies AE,Morris RW,Gibb DB,Cook TA,Morris DL

更新日期：1993-02-01 00:00:00

abstract：:Mouse oocytes were cryopreserved by the vitrification method using vitrification solution (VSI) and the effects of dilution methods were examined on the rate of in vitro and in vivo development. Eighty-three percent and 75% of vitrified oocytes exhibited normal morphology when diluted in glycerol + sucrose and sucrose...

journal_title：Cryobiology

authors： Kono T,Kwon OY,Nakahara T

更新日期：1991-02-01 00:00:00

abstract：:The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events...

journal_title：Cryobiology

authors： Rabin Y,Taylor MJ,Feig JS,Baicu S,Chen Z

更新日期：2013-12-01 00:00:00

abstract：:Malignant mesothelioma (MM) is an aggressive neoplasm usually arising from the mesothelial surfaces of the pleural or peritoneal cavity. Currently, no standard therapy is available. The most commonly used therapy is cytoreductive surgery combined with systematic chemotherapy, but the median overall survival (OS) is le...

journal_title：Cryobiology

authors： Chen J,Liang B,Yuan Y,Liu C,Li L,Li H,Mu F,Zuo J,Xu K

更新日期：2012-12-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...

journal_title：Cryobiology

authors： Marco-Jiménez F,Garzón DL,Peñaranda DS,Pérez L,Viudes-de-Castro MP,Vicente JS,Jover M,Asturiano JF

更新日期：2006-08-01 00:00:00

abstract：:Groups of one hundred Brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. Different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (Me2SO); at different temperatures (0.0±2.0°C and 8.0±2....

journal_title：Cryobiology

authors： Paes Mdo C,da Silva RC,do Nascimento NF,Valentin FN,Senhorini JA,Nakaghi LS

更新日期：2014-12-01 00:00:00

abstract：:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

journal_title：Cryobiology

authors： Hao YJ,Liu QL,Deng XX

更新日期：2001-08-01 00:00:00

abstract：:This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...

journal_title：Cryobiology

authors： Seifi-Jamadi A,Ahmad E,Ansari M,Kohram H

更新日期：2017-04-01 00:00:00

abstract：:We recently published a protocol to vitrify human articular cartilage and a method of cryoprotectant removal in preparation for transplantation. The current study's goal was to perform a cryoprotectant kinetic analysis and theoretically shorten the procedure used to vitrify human articular cartilage. First, the loadin...

journal_title：Cryobiology

authors： Shardt N,Al-Abbasi KK,Yu H,Jomha NM,McGann LE,Elliott JA

更新日期：2016-08-01 00:00:00

abstract：:A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facil...

journal_title：Cryobiology

authors： Caruana M,Battle T,Fuller B,Davidson B

更新日期：1999-03-01 00:00:00

abstract：:The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...

journal_title：Cryobiology

authors： Worland MR,Block W

更新日期：1999-02-01 00:00:00

abstract：:Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechano...

journal_title：Cryobiology

authors： Traversari G,Cincotti A

更新日期：2020-11-27 00:00:00

abstract：:This brief paper introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist. ...

journal_title：Cryobiology

authors： Elliott JA

更新日期：2010-02-01 00:00:00

abstract：:The formation of ice crystals within cells (IIF) is lethal. The classical approach to avoiding it is to cool cells slowly enough so that nearly all their supercooled freezable water leaves the cell osmotically before they have cooled to a temperature that permits IIF. An alternative approach is to cool the cell rapidl...

journal_title：Cryobiology

authors： Mazur P,Pinn IL,Kleinhans FW

更新日期：2007-10-01 00:00:00

abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...

journal_title：Cryobiology

authors： Cheng LY,Sun TC,Liu XC,Yu H,Zhou SJ,Tian L,Yang SH,Liu BX

更新日期：2020-12-03 00:00:00

abstract：:Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...

journal_title：Cryobiology

authors： Bielanski A

更新日期：2005-04-01 00:00:00

abstract：:Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...

journal_title：Cryobiology

authors： Armitage WJ,Juss BK

更新日期：2003-04-01 00:00:00

abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.cryobiol.2009.05.007. The duplicate article has therefore been withdrawn. ...

journal_title：Cryobiology

authors： Wowk B

更新日期：2009-06-06 00:00:00

abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

journal_title：Cryobiology

authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

更新日期：2020-11-26 00:00:00

abstract：:We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...

journal_title：Cryobiology

authors： Kobayashi T,Sumimoto R,Shimada H,Kamada N,Nakagawara G

更新日期：1991-10-01 00:00:00

abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:The process of ice formation and propagation during cryopreservation impacts on the post-thaw outcome for a sample. Two processes, either network solidification or progressive solidification, can dominate the water-ice phase transition with network solidification typically present in small sample cryo-straws or cryo-v...

journal_title：Cryobiology

authors： Kilbride P,Morris GJ,Milne S,Fuller B,Skepper J,Selden C

更新日期：2014-12-01 00:00:00

abstract：OBJECTIVE:Liver metastases occur in approximately 4%-14% of gastric cancer patients and are associated with high mortality. However, no standardized treatment approach is available for these patients. We aimed to assess the clinical outcomes of patients with gastric cancer liver metastases (GCLM) who underwent percutan...

journal_title：Cryobiology

authors： Chang X,Wang Y,Yu HP,Zhang WH,Yang XL,Guo Z

更新日期：2018-06-01 00:00:00

abstract：:We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...

journal_title：Cryobiology

authors： Lewis JG,Learmonth RP,Watson K

更新日期：1994-04-01 00:00:00

abstract：:It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thi...

journal_title：Cryobiology

authors： Möhlmann DT

更新日期：2009-06-01 00:00:00