Abstract:
:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root was enhanced. Eight M26 single-bud sibling lines were used to assess genetic stability. Although cytological examination revealed a ploidy difference in the noncryopreserved control, the ploidy constitution remained relatively stable during the period of cryopreservation. Amplified fragment length polymorphism (AFLP) assay was performed to detect DNA-level variation. No change in DNA fragment pattern and number was observed between the control and the cryopreserved samples. In addition, methylation-sensitive amplified polymorphism (MSAP) assay was carried out to investigate the DNA methylation status during the period of cryopreservation. It was found that cryopreservation induced a decrease in DNA methylation level.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Hao YJ,Liu QL,Deng XXdoi
10.1006/cryo.2001.2339subject
Has Abstractpub_date
2001-08-01 00:00:00pages
46-53issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(01)92339-9journal_volume
43pub_type
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