Abstract:
:The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection. They were then treated with a cryoprotecting mixture containing 8% (v/v) dimethyl sulfoxide (Me2SO) and 20% (w/v) sucrose at 25 degrees C for 1 h followed by cooling at 0.09 degrees C/min to 4 degrees C then cooling to -35 degrees C at 0.72 degrees C/min. Vials containing the root tips were then plunged into liquid nitrogen. After thawing in a water bath to 37 degrees C, root tips were held in the cryoprotecting mixture for 10 min before it was diluted to 25% of its original concentration. Root tips were washed once with fresh liquid White's medium and held for 1 h prior to culturing on White's medium with 0.2% Gelrite and 3% (w/v) sucrose. Regrowth of root tips averaged 65%. Independent variables in this study included 1) Me2SO concentration; 2) the type and concentration of cosolutes; 3) cooling rate(s); 4) the temperature at which the sample is transferred to liquid nitrogen; 5) the age of the culture from which root tips are taken; 6) the recovery period between root tip excision and immersion in cryoprotectant; and 7) the amount of Gelrite used in the postthaw plating medium.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Teoh KH,Weathers PJ,Cheetham RD,Walcerz DBdoi
10.1006/cryo.1996.0011subject
Has Abstractpub_date
1996-02-01 00:00:00pages
106-17issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(96)90011-5journal_volume
33pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract::Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00031-7
更新日期:2002-06-01 00:00:00
abstract:BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2015.07.006
更新日期:2015-10-01 00:00:00
abstract::In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(91)90026-k
更新日期:1991-06-01 00:00:00
abstract::Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2306
更新日期:2001-03-01 00:00:00
abstract::A 2,3-butanediol containing 96.7% (w/w) racemic mixture of the levo and dextro isomers and only 3.1% (w/w) of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 degrees C at rates between 2 and 3500 degrees C/min, followed by slo...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(92)90036-2
更新日期:1992-06-01 00:00:00
abstract::As known for different metabolic functions, α-lipoic acid (ALA) has been tested for spermatozoa preservation of animals as well as of human, but not for fish spermatozoa. The present study determined the effects of ALA on short and long-term (cryopreservation) preservation of common carp (Cyprinus carpio) spermatozoa,...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.04.006
更新日期:2020-06-01 00:00:00
abstract::The effect of cryopreservation on human nasal mucosal biopsies was evaluated by determining the frequency and coordination of the ciliary beat. Samples were cryopreserved in a medium containing 80% Gey's balanced salt solution, 10% dimethyl sulfoxide, and 10% fetal calf serum. After thawing, the samples were put in a ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(85)90164-6
更新日期:1985-04-01 00:00:00
abstract::Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.07.010
更新日期:2008-10-01 00:00:00
abstract::1,3-Butanediol and 2,3-butanediol have been used in buffered solutions with 20, 30, or 35% (w/w) alcohol to cool erythrocytes to -196 degrees C at different cooling rates between 1 to 3500 degrees C/min, followed by slow or rapid rewarming. 1,3-butanediol shows the same shapes of red blood cell survival curves as 1,2-...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90019-3
更新日期:1988-02-01 00:00:00
abstract::Cell volumes, viability, ultrastructure, and metabolism have been studied in mouse L cells at reduced water contents. Dehydration was achieved by addition of sorbitol to the incubation medium at concentrations of 0.15, 0.3, and 0.6 m. In 0.3 m sorbitol, cell volume was reduced to about 35% of the initial control level...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(83)90048-2
更新日期:1983-10-01 00:00:00
abstract::Vitrification is a promising alternative to tissue preservation, in which the tissue is permeated with cryoprotective agents (CPAs) in order to circumvent the hazardous effects associated with ice formation. In this study, we evaluate the effect of vitreous cryopreservation of rabbit trachea, by comparing vitrificatio...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.12.009
更新日期:2009-04-01 00:00:00
abstract::Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.11.001
更新日期:2020-11-10 00:00:00
abstract::The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2006.03.011
更新日期:2006-08-01 00:00:00
abstract::In an experimental study using porcine corneas we investigated the influence of several variables of freeze-thaw trauma on survival of corneal endothelial cells after corneal cryopreservation employing chondroitin sulfate as a cryoprotectant. We examined the influence of: (1) the concentration of chondroitin sulfate i...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1993.1039
更新日期:1993-08-01 00:00:00
abstract::Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The pu...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.04.005
更新日期:2014-06-01 00:00:00
abstract::In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2209
更新日期:1999-11-01 00:00:00
abstract::Collagenase-isolated islets, which had been cultured for 1 week, were frozen at two different cooling rates. Islets frozen at 5 degrees C/min behaved from a functional point of view very similarly to that of nonfrozen, cultured control islets, except for a reduced maximal insulin secretory capacity and a reduced insul...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(83)90005-6
更新日期:1983-04-01 00:00:00
abstract::The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.cryobiol.2009.05.007. The duplicate article has therefore been withdrawn. ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2009.05.006
更新日期:2009-06-06 00:00:00
abstract::Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.05.004
更新日期:2018-08-01 00:00:00
abstract::We investigated cryopreservation of horizontal transmission stages of Holospora undulata, a micronucleus-specific bacterial parasite of Paramecium caudatum. Unlike in previous studies on related Holospora species, protocols using glycerol as cryoprotectant failed entirely. In contrast, freezing with dimethyl sulfoxide...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.10.014
更新日期:2006-02-01 00:00:00
abstract::One thousand cryohemorrhoidectomies were performed in the past 7 years (1973-1979). Ninety percent of the patients were satisfied with the technique; approximately one-third of the patients experienced severe to moderate pain and 11% had recurrences or related problems. The majority of patients with postoperative pain...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(82)90154-7
更新日期:1982-06-01 00:00:00
abstract::A recent study from our laboratory indicated additional tissue injury during rewarming of a cooled rabbit leg. Oxygen-derived free radicals were believed to play a role in such "rewarming injury." Since free radicals may attack membrane phospholipids, we analyzed the phospholipid composition in the leg tissue during c...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(91)90020-o
更新日期:1991-04-01 00:00:00
abstract::The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2011.05.005
更新日期:2011-10-01 00:00:00
abstract::We have shown previously that the survival of human red blood cells during slow freezing at 2% hematocrit is dependent more on the magnitude of the unfrozen fraction than on the salt concentration in that unfrozen fraction. In parallel, first Nei and more recently Pegg and colleagues have shown that survival is affect...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(85)90029-x
更新日期:1985-12-01 00:00:00
abstract::Trehalose, a non-reducing glucose disaccharide found at high concentrations in many species of anhydrobiotic organisms, shows significant promise in protecting cellular viability and structural integrity during freezing and desiccation. As mammalian cell membranes are impermeable to trehalose, extensive efforts have b...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2007.06.003
更新日期:2007-10-01 00:00:00
abstract::All the aqueous solutions of linear saturated polyalcohols with four carbons have been investigated at low temperature. Only ice has been observed in the solutions of 1,3-butanediol and 1,2,3- and 1,2,4-butanetriol. For same solute concentration, the glass-forming tendency on cooling is highest with 2,3-butanediol, wh...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(86)90031-3
更新日期:1986-10-01 00:00:00
abstract::We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(91)90051-o
更新日期:1991-10-01 00:00:00
abstract::Addition of cholesterol-loaded cyclodextrin (CLC) to the diluents of mammalian semen increased stability and rigidity of phospholipid hydrocarbon chains of plasma membrane during sperm cryopreservation process. CLC has been tested successfully as cryoprotectant in various livestock sperm cryopreservation protocols but...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2015.01.009
更新日期:2015-04-01 00:00:00
abstract::Malignant mesothelioma (MM) is an aggressive neoplasm usually arising from the mesothelial surfaces of the pleural or peritoneal cavity. Currently, no standard therapy is available. The most commonly used therapy is cytoreductive surgery combined with systematic chemotherapy, but the median overall survival (OS) is le...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2012.08.003
更新日期:2012-12-01 00:00:00
abstract::The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2221
更新日期:2000-02-01 00:00:00