Cryopreservation of Holospora undulata, a bacterial parasite of the ciliate Paramecium caudatum.

abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...

journal_title：Cryobiology

authors： Lautner LJ,Freed DH,Nagendran J,Acker JP

更新日期：2020-06-01 00:00:00

abstract：:The development of efficient and robust methods for the cryopreservation of human embryonic stem cells (hESCs) is important for the production of master and working cell banks for future clinical applications. Such methods must meet requirements of good manufacturing practice (GMP) and maintain genetic stability of th...

journal_title：Cryobiology

authors： Amps KJ,Jones M,Baker D,Moore HD

更新日期：2010-06-01 00:00:00

abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...

journal_title：Cryobiology

authors： Cheng LY,Sun TC,Liu XC,Yu H,Zhou SJ,Tian L,Yang SH,Liu BX

更新日期：2020-12-03 00:00:00

abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...

journal_title：Cryobiology

authors： Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JC

更新日期：2001-02-01 00:00:00

abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

journal_title：Cryobiology

authors： Wang HS,Kang L

更新日期：2005-10-01 00:00:00

abstract：:In order to preserve genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated. Survival rates under various conditions were compared: the nature and the concentration of cryoprotectants before and after freezing, the seeding temperature, and...

journal_title：Cryobiology

authors： Kusuda S,Teranishi T,Koide N

更新日期：2002-08-01 00:00:00

abstract：:The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...

journal_title：Cryobiology

authors： Worland MR,Block W

更新日期：1999-02-01 00:00:00

abstract：:(1) Tyrosine and tryptophan metabolism in brain and peripheral tissues were studied in hypothermic hibernating and normothermic nonhibernating 13-lined ground squirrels (Spermophilus tridecemlineatus). (2) In the hypothermic hibernating state, there were significant elevations of brain stem tyrosine, norepinephrine, a...

journal_title：Cryobiology

authors： Weekley BL,Harlow HJ

更新日期：1987-12-01 00:00:00

abstract：:Ibex spermatozoa can be successfully frozen using glycerolated media. However, no information is available regarding the most effective method of glycerol addition in this species. The aim of the present work was to evaluate the effect of the glycerolization temperature on the response to freezing-thawing of ibex sper...

journal_title：Cryobiology

authors： Coloma MA,Gómez-Brunet A,Velázquez R,Toledano-Díaz A,López-Sebastián A,Santiago-Moreno J

更新日期：2010-10-01 00:00:00

abstract：:Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...

journal_title：Cryobiology

authors： Browne RK,Mahony M,Clulow J

更新日期：2002-06-01 00:00:00

abstract：:The cryopreservation of isolated fish blastomeres is likely to provide a valid alternative to embryo cryopreservation, the results of which are still discouraging. A repeatable technique for the cryopreservation of rainbow trout blastomeres has been established and the effect of embryonic developmental stage on freezi...

journal_title：Cryobiology

authors： Calvi SL,Maisse G

更新日期：1998-06-01 00:00:00

abstract：:Semen was collected in the laboratory from nine healthy donors. The concentrations and the percentages of live and motile spermatozoa in all semen samples were within the normal range. Each sample was diluted with citrate-egg yolk-glycerol medium with and without 5 mM dithiothreitol (DTT). Samples were frozen in liqui...

journal_title：Cryobiology

authors： Rao B,David G

更新日期：1984-10-01 00:00:00

abstract：:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34...

journal_title：Cryobiology

authors： Miura J,Minegishi M,Itoh T,Kitaura T,Fukawa N,Takahashi H,Suzuki A,Kudo Y,Narita A,Sato Y,Suzuki M,Wada Y,Takeyama Y,Watanabe T,Tsuchiya S

更新日期：2008-10-01 00:00:00

abstract：:Groups of one hundred Brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. Different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (Me2SO); at different temperatures (0.0±2.0°C and 8.0±2....

journal_title：Cryobiology

authors： Paes Mdo C,da Silva RC,do Nascimento NF,Valentin FN,Senhorini JA,Nakaghi LS

更新日期：2014-12-01 00:00:00

abstract：:In the determination of the solid-liquid phase equilibria in the aqueous mixtures of dimethyl sulfoxide (Me2SO) and ethylene glycol (EG) one often encounters the problem of equilibrium crystallization. In the present report the above aqueous solutions are equilibrated for crystallization in a dielectric cell during wh...

journal_title：Cryobiology

authors： Murthy SS

更新日期：1998-03-01 00:00:00

abstract：:Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...

journal_title：Cryobiology

authors： Du Y,Zhang Y,Li J,Kragh PM,Kuwayama M,Ieda S,Zhang X,Schmidt M,Bøgh IB,Purup S,Pedersen AM,Villemoes K,Yang H,Bolund L,Vajta G

更新日期：2007-04-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

journal_title：Cryobiology

authors： Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

更新日期：1989-04-01 00:00:00

abstract：:One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

journal_title：Cryobiology

authors： Wang CL,Teo KY,Han B

更新日期：2008-08-01 00:00:00

abstract：:Cryopreservation procedures cause osmotic stress to spermatozoa following cryoinjury and reduce their content of osmolytes. Conventional method for cryoprotectant loading and dilution on canine semen freezing which could be categorized in single step protocol, makes decreasing in sperm performance such as motility, mo...

journal_title：Cryobiology

authors： Setyawan EM,Kim MJ,Oh HJ,Kim GA,Jo YK,Lee SH,Choi YB,Lee BC

更新日期：2015-10-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....

journal_title：Cryobiology

authors： Zeron Y,Tomczak M,Crowe J,Arav A

更新日期：2002-10-01 00:00:00

abstract：:Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...

journal_title：Cryobiology

authors： Bielanski A

更新日期：2005-04-01 00:00:00

abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...

journal_title：Cryobiology

authors： De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

更新日期：1998-09-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...

journal_title：Cryobiology

authors： Paynter SJ,Fuller BJ,Shaw RW

更新日期：1999-09-01 00:00:00

abstract：:Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disacchari...

journal_title：Cryobiology

authors： Chakraborty N,Menze MA,Elmoazzen H,Vu H,Yarmush ML,Hand SC,Toner M

更新日期：2012-04-01 00:00:00

abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...

journal_title：Cryobiology

authors： Hunter JE,Bernard A,Fuller BJ,McGrath JJ,Shaw RW

更新日期：1992-04-01 00:00:00

abstract：:The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...

journal_title：Cryobiology

authors： Arnaud FG,Khirabadi B,Fahy GM

更新日期：2003-06-01 00:00:00

abstract：:In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...

journal_title：Cryobiology

authors： Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

更新日期：2009-12-01 00:00:00